...developed photoconvertible FPs like PsMOrange and mEos add another layer of complexity to imaging, as they...encountered by some investigators when using forms of mEos." The ability to repurpose GFP is great news for...

...photoconvertible proteins are PA-GFP, Dendra2, and the mEOS proteins. Some proteins are reversibly switchable...

... Tandem UV-Violet Green/ Red N/A High 569/ 581 mEos Monomer UV-Violet Green/ Red N/A High 573/ 584 ... conditions- mEos4a and mEos4b (12). Both variants are more monomeric than mEos2 (mEos4b is completely.... green to red, or cyan to green). For example, mEosFP (named after the goddess of dawn in Greek mythology...mythology) switches from green to red fluorescence. mEosFP exposure to UV light causes an irreversible cleavage... over the monomeric one since the formation of mEosFP’s chromophore requires a temperature below 30°C ...Dendra2 is another monomeric PA-FP which unlike mEosFP, can form it’s chromophore at a more comfortable...

...20 min Monomer mEos3.2-N1 - Mammalian Expression mEos3.2-C1 - Mammalian Expression mEos4a 505 / 569 516...oligomerization mEos4a-N1 - Mammalian Expression mEos4a-C1 - Mammalian Expression mEos4b 505 / 569 516 ...Expression mEos2 506 / 571 516 / 581 47 / 30 5.6/6.4 Prone to oligomerization pRSETa mEos2 - Bacterial...Bacterial Expression pFA6a-link-yomEos2-CaURA3 - Yeast Expression mEos3.2 507 / 572 516 / 580 53 / 18 5.4/5.8...516 / 581 Monomer mEos4b-N1 - Mammalian Expression mEos4b-C1 - Mammalian Expression dEos 505 / 569 516 /...

...variant (CyPet) found in pCyPet-His . pRSETa mEos2 20341 mEos2 (fluorescent protein) Loren Looger Photoactivable...Photoactivable Fluorescent Protein Expression: contains mEos2 with an N-terminal His tag. Photoactivable fluorescent...

...recipient institution. An authorized signatory is someone who can execute legal documents on behalf of the...

...receptors play a key role in development and homeostasis, and the receptors and their downstream genes...

...particularly true if you receive a plasmid from someone in another lab, or dig one out of the freezer and...

...specific instructions so be sure to check in with someone familiar with your lab’s instrument before use....

...FRET sensors to monitor intracellular Zn(2+) homeostasis. Nat Methods. 2009 Oct;6(10):737-40. Maarten ...

... you see someone who gives a great talk, ask them for advice on speaking. If you see someone with a great...circle. Asking someone to be your mentor Mentoring is a “loaded” word.  If you ask someone to be your mentor...some of the many ways you can approach finding someone to give you advice and guidance. Second, I will...advice on how to “make the ask” once you have found someone you admire and want to learn from. Check out Joanne's...whose career path you are interested in following? Someone you admire for their insight and way of expressing...making the pledge to have lunch or coffee with someone I don’t know very well at least twice a week. I... choose one that comes highly recommended from someone you trust. A good coach can make a big difference...

...give someone a card they are more likely to remember you if you follow up and if you give someone your... to someone and introducing yourself. Put your phone away! This is a natural time to meet someone since... to try and meet. Don’t worry about contacting someone much more senior than you – meeting scientists ...conference may start in the airport. If you see someone traveling with a poster roll, say hello. Keep your...people learn names by seeing them. It is OK to ask someone to see their name tag if you are one of those people...memorable. It isn’t always appropriate to walk up to someone much more senior than you, but you can safely seek...

...doled out to multiple people: someone to coordinate, someone to decide, someone to delegate, etc. If you find... find that there is not someone (or someones) taking on these responsibilities on a team to which you ... subtle but important distinction. If I invite someone to be on a project team, I try to make it very ...kind of team, and you have nagging doubts that someone else on that team is putting their own self-interest...

... your CV or business card. Be warned, though – someone accepting your CV doesn’t necessarily mean that...have a mentor? There is a difference. A mentor is someone who has gone where you hope to go, and who will...made at a conference or job fair, or it may be someone at your institution’s career center. Alternatively...colleague. A mentor does not necessarily need to be someone a lot older than you are; try using ResearchGate... a job! This is good etiquette when contacting someone professionally that you do not know very well; ...

...project. You’ve met someone whose career interests you or you want to pursue someone doing a job you wish...potential to alienate connections. If meeting with someone to ask about a job or a particular position at ...interest in a job. Ask openly to be connected to someone who can help you land that position such as a direct...

...Parkinson's, FTD Michael Davidson 57468 mEos3.2-MAPTau-N-10 MAPT mEos3.2 CMV Parkinson's, FTD Michael Davidson...

...Relationships need nurturing. Perhaps you’ve met someone interesting at an event. Or, perhaps you have a...appreciation. Send a thank you note or gift to someone who helped you succeed in any way. Don’t believe...strawberries for helping with an introduction that helped someone get a job interview. Even just a thank you email...is having a mutual acquaintance connect you to someone who you need to speak to for informational or career...

...relationship. Pro tip: Once you are connected with someone, their email address is usually visible to you ...if you don’t know clearly why you are inviting someone to connect, perhaps you should be asking yourself...discussions.  Pro tip: Instead of connecting with someone you don’t really know, follow instead of connecting...do an easy favor for people in your network. If someone has connected with me, I consider them fair game...

...introduction. During the conference When you meet someone new at a conference, one of the first things you...poster session neighbors. During the session, if someone comes up to my poster, I usually greet them and...scientists attending. Reach out if you’d like to meet someone and set aside a time to meet. Some conferences ...policies before posting something on social media. If someone is presenting unpublished data they may ask the...

...being a manager: yes, being a manager means being someone’s boss. But it does not mean shouting orders and...throughout the year and in a timely manner. If someone’s performance or behavior is inadequate, you need...equally as important so don’t forget to recognize someone’s hard work and reward a job well done. Your report...to your manager. This is when having a mentor - someone more experienced to guide you - is valuable. Protect...