...Antigen-1 (oriP-EBNA-1) system where each vector contains a viral origin of DNA replication, OriP and the...an extra plasmid, pCXWB-EBNA-1 is used in the reprogramming cocktail. pCXWB-EBNA-1 lacks oriP and is unable...and to model diseases in vitro. Find stem cell plasmids at Addgene Many sources of adult somatic cells...Episomal vectors- extrachromosomally maintained DNA plasmids that express reprogramming factors To date, the...in human cells [8]. The EBNA-1 protein recognizes OriP and induces amplification of the episomes concurrently...group is an optimized mixture of three episomal plasmids (Table 1) encoding the reprogramming factors. ...increases protein expression from the other episomal plasmids. When used on PBMCs, the pCXLE episomal vector...

...into cells as a donor plasmid containing the transposon, along with a helper plasmid that expresses the ...reprogramming factors. Non-replicating Plasmids: Generating iPSCs with plasmid-based expression requires serial... generating iPSCs by plasmid transfection where 2 of 11 clones tested had plasmid integration. Another...the dose of plasmid the cells receive over the whole reprogramming period and the plasmid will get diluted...larger plasmids have lower rates of transfection. Minicircles: Minicircles are like mini-plasmids. They... Replicating oriP/Epstein-Barr nuclear antigen-1 (EBNA1) based episomes: These plasmids carry the origin...replication (oriP) element and the cis-acting EBNA-1 from Epstein-Barr virus. EBNA-1 binds oriP and allows...