...based on p-element, random or targeted, integration into the fly genome and are under the Gal4 inducible... to insert YGOI, a bacterial selection cassette for growth and cloning in bacteria and a mammalian selection...accessory packaging vectors to generate the virus. Bacterial vectors This system is great for overexpressing...isolated, controlled environment. In most cases bacterial expression vectors are inducible (e.g. pBAD LIC...

...fruit fly is the array of genetic tools, such as the GAL4/UAS and LexA system, that allows scientists to easily...systems but can be quite difficult and time consuming. GAL4/UAS was first described in 1993 by Norbert Perrimon...PubMed Central PMCID: PMC5349099. Nguyen, Jeffrey P., et al. "Whole-brain calcium imaging with cellular...cancer since mice better recapitulate the complex interactions between cancer cells, therapeutic drugs, and...mice to study how different mutations in leukemia impact different treatment regimens (Zuber et al., 2009... model organisms is that they are genetically tractable. Mice can be easily manipulated with tools like...not only for the reasons above but because they actually share many biological properties and processes...

...original Y2H systems utilized the yeast Gal4 activator, bacterial LexA DBD and the lacZ reporter gene (3...Chen, Y.C., Rajagopala S.V., Stellberger T., Uetz P.  Exhaustive Benchmarking of the yeast two-hybrid ...668. Pubmed PMID: 20805792. 7. Stynen B., Van Dijck P., Tournu H. A CUG codon adapted two-hybrid system ...20719741. Pubmed Central PMCID: PMC2965261. 8. Obrdlik, P., El-Bakkoury, M., Hamacher, T., Cappellaro, C., Vilarino...molecular interactions in the cell, including protein-protein, protein-DNA and protein-RNA interactions. The...transcriptional activators: that the DNA binding domain (DBD) and transcriptional activation (TA) domains...GFP), regardless of whether it interacts with a transcriptional activator (1a) or a repressor (1b). Scientists...