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We narrowed to 9 results for: p65

Showing: 1 - 9 of 9 results
  1. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...then recruit additional activation domains (HS1 and p65). Performance When targeting single genes, SAM consistently... as two other potent transcriptional activators (p65 and Rta). These transcriptional activators work in...activators, fusing the epitope tag of SunTag with the P65-HSF activation domains used in SAM. This method has...
  2. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...by the Yang Lab with the development of a Suntag-p65-HSF1 (SPH) transgenic mouse (Zhou et al., 2018). ...activator that recruits up to twenty copies of the p65-HSF1 transcriptional activator to each genome-bound...
  3. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...fused to a transcriptional activator domain (VP64 or p65). Upon expression in the cell, the dCas9-CIB1 fusion...combination was NLS-dCas9-trCIB1 and NLSx3-CRYPHR-p65 - it had the lowest background activity in the dark...they fused BldD to the transcriptional activators p65 and VP64 which in turn transcribes N-Cas9. The N-...
  4. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...binding protein (a portion of CRY2) fused to the p65 activation domain, which activates transcription ...below. Scientists can obtain dCas9-trCIB1, CRY2PHR-p65, and other plasmids from this article through Addgene...although three of the additional domains tested (VP64, p65, and Rta) were capable of increasing target gene ...three domains onto dCas9 to generate a dCas9-VP64-p65-Rta fusion protein (referred to as dCas9-VPR) had...
  5. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...is set as 1. Some proteins are very dim (e.g. TagRFP657, which has a brightness of 0.1) and this should...
Showing: 1 - 9 of 9 results