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We narrowed to 14 results for: p65

Showing: 1 - 14 of 14 results
  1. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ...then recruit additional activation domains (HS1 and p65). Performance When targeting single genes, SAM consistently... as two other potent transcriptional activators (p65 and Rta). These transcriptional activators work in...activators, fusing the epitope tag of SunTag with the P65-HSF activation domains used in SAM. This method has...
  2. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...by the Yang Lab with the development of a Suntag-p65-HSF1 (SPH) transgenic mouse (Zhou et al., 2018). ...activator that recruits up to twenty copies of the p65-HSF1 transcriptional activator to each genome-bound...
  3. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...fused to a transcriptional activator domain (VP64 or p65). Upon expression in the cell, the dCas9-CIB1 fusion...combination was NLS-dCas9-trCIB1 and NLSx3-CRYPHR-p65 - it had the lowest background activity in the dark...they fused BldD to the transcriptional activators p65 and VP64 which in turn transcribes N-Cas9. The N-...
  4. Lentiviral Prep Service

    Type
    Collection
    ...backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) targeting...
  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...binding protein (a portion of CRY2) fused to the p65 activation domain, which activates transcription ...below. Scientists can obtain dCas9-trCIB1, CRY2PHR-p65, and other plasmids from this article through Addgene...although three of the additional domains tested (VP64, p65, and Rta) were capable of increasing target gene ...three domains onto dCas9 to generate a dCas9-VP64-p65-Rta fusion protein (referred to as dCas9-VPR) had...
  6. Zhang Lab CRISPR Page

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    Collection
    ...aptamers at the tetraloop and stemloop 2 The MS2-P65-HSF1 activation helper protein Full references are... activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator helper complex with 2A GFP 61424 :...marker 61426 : lentiviral vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance...
  7. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Bacterial Expression TagRFP657 611 657 0.34 5.3 27 min Prone to dimerization pTagRFP657-N1 - Mammalian Expression...Expression pTagRFP657-C1 - Mammalian Expression pFA6a-link-yoTagRFP657-Kan - Yeast Expression smURFP 642...
  8. Immunology Research Plasmids and Resources

    Type
    Collection
    ...viral oncogene homolog A (avian) MGC131774, NFKB3, p65 SYK spleen tyrosine kinase DKFZp313N1010, FLJ25043...viral oncogene homolog A (avian) MGC131774, NFKB3, p65 RHOA ras homolog gene family, member A ARH12, ARHA...B-cell linker BASH, BLNK-S, LY57, MGC111051, SLP-65, SLP65 BTK Bruton agammaglobulinemia tyrosine kinase AGMX1...
Showing: 1 - 14 of 14 results