... protocols call for paraformaldehyde (PFA) as the fixative. PFA is a polymer of formaldehyde that, on ...on its own, is not a fixative. However, when PFA is dissolved in solution and heated, it depolymerizes,...formaldehyde. Often people will call the resulting solution PFA, but it’s really just formaldehyde. Formalin: You...

...usually test 3 different fix/perms (methanol, PFA-saponin, PFA-triton). The fix/perm solutions can make a... of your antibody. When compared, 4% PFA and 0.1% saponin; 4% PFA and 0.1% Triton X-100; and methanol,...have found that some antibodies perform well in a PFA-saponin fixation and permeabilization protocol, but... cells were mixed in a 1:1 ratio and fixed in 4% PFA and permeabilized in 0.1% saponin. 400,000 cells ...

...ΔΔCT No Standard Curve reference=target Pfaffl et al No Standard Curve sample=control ...Hellemans J; Huggett J; Kubista M; Mueller R; Nolan T; Pfaffl MW; Shipley GL; Vandesompele J; Wittwer CT. The...2002) 119 – 133Tichopad A; Dilger M; Schwarz G; Pfaffl MW. 2003. Standardized determination of real-time...

...tagging vectors are based on previously described pFA6a-link vectors and include a Kan, SpHIS5, or CaURA3...

...searched for Argonaute orthologs similar to TtAgo and PfAgo, another thermophilic Argonaute. They identified...

...red) 14.9 (blue), 6.8 (red) 2.8/4.1 7.1 h (red) pFast-FT-N1 pTRE-Fast-FTpBAD/HisB-Fast-FT Medium-FT...