... protocols call for paraformaldehyde (PFA) as the fixative. PFA is a polymer of formaldehyde that, on ...on its own, is not a fixative. However, when PFA is dissolved in solution and heated, it depolymerizes,...formaldehyde. Often people will call the resulting solution PFA, but it’s really just formaldehyde. Formalin: You...

...usually test 3 different fix/perms (methanol, PFA-saponin, PFA-triton). The fix/perm solutions can make a... of your antibody. When compared, 4% PFA and 0.1% saponin; 4% PFA and 0.1% Triton X-100; and methanol,...have found that some antibodies perform well in a PFA-saponin fixation and permeabilization protocol, but... cells were mixed in a 1:1 ratio and fixed in 4% PFA and permeabilized in 0.1% saponin. 400,000 cells ...

...ΔΔCT No Standard Curve reference=target Pfaffl et al No Standard Curve sample=control ...Hellemans J; Huggett J; Kubista M; Mueller R; Nolan T; Pfaffl MW; Shipley GL; Vandesompele J; Wittwer CT. The...2002) 119 – 133Tichopad A; Dilger M; Schwarz G; Pfaffl MW. 2003. Standardized determination of real-time...

...tagging vectors are based on previously described pFA6a-link vectors and include a Kan, SpHIS5, or CaURA3...

...searched for Argonaute orthologs similar to TtAgo and PfAgo, another thermophilic Argonaute. They identified...

...red) 14.9 (blue), 6.8 (red) 2.8/4.1 7.1 h (red) pFast-FT-N1 pTRE-Fast-FTpBAD/HisB-Fast-FT Medium-FT...

...-GFP-KanR - Yeast Expression pFA6a-PA-GFP-kanMX6 - Yeast Expression pFA6a-link-yEPAGFP-CaUra3 - Yeast ...dimerization pcDNA3-Clover - Mammalian Expression pFA6a-link-yoClover-Kan - Yeast Expression dClover2 505...Tandem-dimer pCSCMV:tdTomato - Mammalian Expression pFA6a-link-tdTomato-SpHis5 - Yeast Expression tdTomato-N1...dimerization pcDNA3-mRuby2 - Mammalian Expression pFA6a-link-yomRuby2-SpHis5 - Yeast Expression mRuby2-N1...cloning vector (u-mCherry) - Bacterial Expression pFA6a-link-yomCherry-SpHis5 - Yeast Expression mCherry2...Expression pTagRFP657-C1 - Mammalian Expression pFA6a-link-yoTagRFP657-Kan - Yeast Expression smURFP +...T-sapphire 399 511 26 4.9 1.3 hr Prone to dimerization pFA6a-link-yETSapphire-CaUra3 - Yeast Expression mT-sapphire...

...expression under the metallothionein promoter pFastBac LIC and pFastBac Dual LIC - Vectors from the Gradia lab...cerevisiae cells His-tagged versions of pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression...gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope tagging in S. pombe Insect/Baculovirus... TEV-His6-Flag for bacterial expression pFA6a vectors - PCR-based C-terminal tagging in S. cerevisiae...N-terminal GST tag fusion with your gene of interest pFastbac1 Nterminal GST - Baculovirus expression vector ...

... FUS_LC_withR FUS T7 ALS Nicolas Fawzi 139211 pFastBac1-LRRK2 WD40 LRRK2 His, MBP polH Parkinson's Hao...S473D OPTN GST-TEV ALS Sascha Martens 187830 pFastBac_Dual_GST-TEV-EGFP-TBK1 TBK1 GST, TEV-mEGFP-GG Linker...6His HTRA2 His T7 Parkinson's Eric Fischer 198033 pFastBac-GST-TEV-mCherry-TBK1 TBK1 mCherry, GST, Tev P10...P10 ALS Sascha Martens 198037 pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K TBK1 GFP, GST, Tev P10 ALS Sascha ...Sascha Martens 198072 pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666) TBK1 GFP, GST, Tev P10 ALS Sascha Martens...) OPTN GFP, FKBP CMV ALS Sascha Martens 208875 pFastBac-Dual- GST-TEV-TBK1 TBK1 polH ALS Sascha Martens...

...fluorescent proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al., 2004; Lee et al., 2013...