...lentiviral expression, we could use a vector like pLenti CMV Puro DEST (w118-1) or the doxycycline-inducible pLIX...vector systems. With the appropriate entry and destination vectors, one can use Gateway to clone a gene ...generated entry clone and the attR sites of the destination vector. This reaction is catalyzed by the LR ...containing the ccdB gene is excised from the destination vector.  Once the BP and/or LR reactions are ...select the positive clones. The entry clone and destination vector carry different antibiotic resistance ...gene is present in the donor vectors and the destination vectors prior to recombination, and it is exchanged...expression clone, it is important to choose the destination vector that best fits your experiment. This choice...