We narrowed to 22 results for: polya

... both polyadenylation and termination. Out of those listed, the SV40 late polyA and rbGlob polyA are thought... polya...purpose and mechanism of polyadenylation vary across cell types, but polyadenylation generally serves to promote... and polyadenylation enzymes, in a process that seems to couple termination with polyadenlyation. Mammalian...working with plasmids! What are termination and polyadenylation? The role of the terminator, a sequence-based...after any 3’ regulatory elements, such as the polyadenylation or poly(A) signal. While many studies focus...half-life, and ultimately gene-expression. Polyadenylation is the post-transcriptional additional of multiple...

...just upstream of the final ITR.  polyA  The polyadenylation or polyA signal aids in the nuclear export...translation, and promotes RNA transcript longevity. The polyA is located at the end of the AAV genome, just upstream...

...CREATE cap plasmid, loxP sites flank the downstream polyA sequence. When viruses produced from this plasmid...into an animal with cell-type-specific Cre, the polyA sequence is inverted only in the Cre-expressing ...

...vectors (LTRs and ITRs), plasmids with multiple polyA sites, and other plasmids that contain repeating...

... before considering the need for some manner of polyA. In this scenario, you may be best served by a dual...

...contains a promoter, the gene of interest, and a polyA signal. Created with biorender.com. ITR structure...

...promoter (to drive high-level expression) and a polyA signal (to terminate transcription) and the four...fluorescent proteins, promoters, enhancers, polyadenylation signals, inducible gene-expression sequences...polycistronic containing multiple identical polyadenylation signals or promoters Gibson et al., 2009 ...

...which contains a promoter, the envelope gene, and a polyA tail.   Figure 1: Lentiviral production uses...

...Modular by design: Key elements (promoter, tag, polyA site) are flanked by unique restriction sites so...

...guide to working with Drosophila Terminators and PolyA Signals FLEx Vectors Resources at Addgene.org ...

...constitutive promoters, 2 includible promoters, 3 polyA terminators and various pieces for inducible expression... a duplicated SV40 polyadenylation region instead of the synthetic polyadenylation signal present in pGL3...

... protein mix by size Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is the first...transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic...) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and...

...and Bester 2021). For example, inserting a polyadenylation signal downstream of the transcription start...fragment. (D) Knock-in: insertion of a synthetic polyadenylation (spA) signal. Figure and caption adapted from...

...gene start) and a transcription termination/polyadenylation signal sequence (gene end). A minigenome is..., where it will terminate transcription and polyadenylate the mRNA. These mRNAs are translated by cellular...

...ladder works well for both agarose and native polyacrylamide gels. Get Tips on Verifying Your Plasmid ...

...Jensen New serotype pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA AAV9, PHP.eB Controls Feng New viral service, ...

...proteins by atomic force microscopy (AFM). Maybe polyantibodies could help in the capture of other rare cell...

... C. elegans 3’UTRs structures, alternative polyadenylation for all protein coding mRNAs, and annotations...

...donor site; SAS, splice acceptor site; pA, polyadenylation signal. Image reused from Riedmayr et al. 2023...

...Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver...