We narrowed to 8 results for: puc

1. Plasmids 101: Origin of Replication

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   Blog Post

   Published

   Nov. 11, 2020, 2:23 p.m.

   ...pMB1 ori maintains about 20 copies per cell, while pUC – which differs by only two mutations – will produce...Copy Number+ ori Incompatibility Group Control pUC ~500-700 pMB1 (derivative) A Relaxed pBR322 ~15... (derivative) and F1** A Relaxed pGEM ~300-500 pUC and F1** A Relaxed pCDF ~20-40 CloDF13 (CDF) D...

2. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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   Blog Post

   Published

   March 19, 2019, 1:31 p.m.

   ... 4 kits also contain the two entry vectors pUC-L1L4 and pUC-L3L2. Find the 2in1 plasmid kits at Addgene...cloning system contains two entry vectors (pUC57-L1L4 and pUC57-L3L2) that offer the advantage of using ...

3. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

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   Blog Post

   Published

   Oct. 29, 2015, 2:30 p.m.

   ...and new cloning vectors such as pBR322, pACYC, and pUC were developed to provide higher copy number vectors...

4. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   Blog Post

   Published

   Sept. 4, 2018, 12:53 p.m.

   ...number of plasmids per cell increases 4-5 fold (for pUC plasmids) or ~2 fold (for p15a, R6K, and ColE2 plasmids...

5. Plasmids 101: Dimers and Multimers

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   Blog Post

   Published

   Jan. 10, 2023, 2:15 p.m.

   ...phase growth. In a study by Williams et al. (2009), pUC multimerization was reduced by growing seed stocks...

6. Plasmids 101: Blue-white Screening

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   Blog Post

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   June 4, 2015, 1:03 p.m.

   ... White Screening Available at Addgene Are: pUC18 and pUC19 Let’s begin at the beginning. The well-characterized...the α-complementation cloning MCS): pGEM-T, pUC18 and pUC19, and pBluescript are a few common vectors. ...

7. 28 Hot Plasmid Technologies from 2015

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   Blog Post

   Published

   Dec. 23, 2015, 3:30 p.m.

   ...insert in pUCXKT will therefore have resistance to both kanamycin and ampicillin (from the pUC19 backbone...100% cloning efficiency and gene expression. The pUCXKT vector ensures that all screened plasmids contain...resistances, restriction enzymes and plasmid backbones. pUCXKT contains a nonfunctional truncated version of the...expression of this resistance. Unlike previous systems, pUCXKT does not result in a translational fusion of the...restriction site (the MCS has several options) and a pUCX-specific reverse primer containing the missing codons...product is then ligated into the MCS/SacI site in pUCXKT, removing the stop codons during backbone digestion...

8. 22 Hot Plasmid Technologies from 2014

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   Blog Post

   Published

   Jan. 6, 2015, 5:21 p.m.

   ...plant terminator, and plant resistance cassette) in pUC19 based entry vectors, as well as the pGreen-IIS based...