...Precious Tools to Improve Gene Modification of Hematopoietic Cells for Research and Gene Therapy. Viruses 12...To deliver genes using lentiviral vectors, you need an envelope protein on the virus’s surface and a ... plasmid which contains a promoter, the envelope gene, and a polyA tail.   Figure 1: Lentiviral...can’t be used for neuronal tracing or peripheral gene delivery. Combining the rabies glycoprotein into...Lentiviral Vectors: One Vector, Many Guises. Human Gene Therapy Methods 28:291–301 . https://doi.org/10.1089...Resources on Addgene.org: Check out the lentivirus guide Find ready-to-use lentiviral preps at Addgene ...your viral vector, you use the same protocol for generating lentivirus, but you use a different envelope ...