...kinase with a similar 3D structure to MEK1, but Raf1 and CDK5 have less similarity. For these three kinases...MEK. The photoswitchable versions of MEK1, MEK2, Raf1, and CDK5 all behave similarly to their endogenous...examined the ability of kinase inhibitors to alter Raf1-MEK-ERK pathway signaling by using psMEK1 and an...inhibitor targeted upstream members of the pathway (Raf1, EGRF), the KTR localized to the cytoplasm. This...Phosphorylation of MEK1/2 peaks at just 5 minutes post-Raf1 inactivation, and then decreases. Treatment of the...creating a short pulse of psRaf1 activity. They did this by exposing psRaf1 to a short 2 minute pulse ...500 nm light (turn on psRaf1) followed by 3 seconds of 400 nm light (turn off psRaf1). After this, the phosphorylation...

...able to develop photoswitchable Intersectin, Cdc42, Raf1, Mek1, Mek2, Cdk5, and Cas9. Xin has demonstrated...