...created SRIRACCHA in 2017 to enrich and identify desired CRISPR mutants (Wen et al., 2018). SRIRACCHA uses...Madison lab also created an inducible SRIRACCHA system (iSRIRACCHA) where Cas9 expression is activated ...focus on two fluorescent CRISPR reporter methods, SRIRACCHA and GEMCherry2. We will cover single base-pair...editing CRISPR reporters in the near future.  SRIRACCHA (stable, but reversible, integrated reporter for...genome of mammalian cells.  Figure 1: The SRIRACCHA reporter construct. Image from Wen et al., 2017...compared to unsorted cells (Wen et al., 2018). Thus SRIRACCHA enables scientists to both quantify Cas9 activity...you with just your desired genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric...

... many different phylogenetic groups (Jain and Srivastava 2013).  Recombinant vectors that are made from...transferred to various experimental hosts (Jain and Srivastava 2013). They are also easily-shareable tools within...many different bacteria (Meyer 2009; Jain and Srivastava 2013).  The plasmid does not have many restriction...Schmidhauser et al. 1988; Lale et al 2011; Jain and Srivastava 2013). Conveniently, these broad host range plasmids...Information sourced from Lale et al 2011 and Jain and Srivastava 2013. Family Replicon Compatible with.../doi.org/10.1074/jbc.273.14.8447  Jain, A., & Srivastava, P. (2013). Broad host range plasmids. FEMS Microbiology...

...receptor conformation and signaling activity​​ (Sridharan et al., 2014). It's also crucial to evaluate how...considerations regarding the choice of fluorescent dyes (Sridharan et al., 2014). Manufacturers will typically recommend...1516–1526. https://doi.org/10.1039/c8en01381k Sridharan, R., Zuber, J., Connelly, S. M., Mathew, E., &...

...in Candida albicans A new CRISPR interference (CRIPSRi) platform for targeted gene repression in Candida...

.../doi.org/10.1007/s40259-017-0234-5  Büning H, Srivastava A (2019) Capsid Modifications for Targeting and...

...parasitoid wasps; and an essay on the life of Srinivasa Ramanujan, to be precise) and remarking to a classmate...

... Christine Mummery, Lorenz Studer, and Deepak Srivastava--addressed a battery of questions regarding topics...

...CRISPR regions of the prokaryotic genome. Then he desribed how P. furiosus has systems to defend against ...

...Central PMCID: PMC552224. 4. Gustafsson, Claes, Sridhar Govindarajan, and Jeremy Minshull. "Codon bias ...

.../mmbr.67.1.16-37.2003 Khatodia S, Bhatotia K, Passricha N, Khurana SMP, Tuteja N (2016) The CRISPR/Cas...

...PMCID: PMC393301. Da Silva, Nancy A. & Sneha Srikrishnan. “Introduction and expression of genes for metabolic...

...B978-012198382-6/50026-1 Wang R, Xiang S, Feng Y, Srinivas S, Zhang Y, Lin M, Wang S (2013) Engineering production...

..., J. S., Button, D., Wang, Y., Dufour, B. D., Srinivasan, S., & McBride, J. L. (2019). AAV-PHP.B Administration...