Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
Type
Blog Post
...created SRIRACCHA in 2017 to enrich and identify desired CRISPR mutants (Wen et al., 2018). SRIRACCHA uses...Madison lab also created an inducible SRIRACCHA system (iSRIRACCHA) where Cas9 expression is activated ...focus on two fluorescent CRISPR reporter methods, SRIRACCHA and GEMCherry2. We will cover single base-pair...editing CRISPR reporters in the near future.
SRIRACCHA (stable, but reversible, integrated reporter for...genome of mammalian cells.
Figure 1: The SRIRACCHA reporter construct. Image from Wen et al., 2017...compared to unsorted cells (Wen et al., 2018). Thus SRIRACCHA enables scientists to both quantify Cas9 activity...you with just your desired genomic edit.
Find SRIRACCHA plasmids at Addgene
GEMCherry2, an improved monomeric...