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We narrowed to 25 results for: tetracycline

Showing: 1 - 20 of 25 results

  1. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...reverse tetracycline-controlled transactivator) is normally inactive and cannot bind the tetracycline response... inducible promoters. The positive inducible tetracycline ON (Tet-On) system, a versatile tool developed...response elements (TRE) in a promoter. Tetracycline and its derivatives serve as inducing agents to allow...Addgene.org: Inducible Promoters for Bacteria Tetracycline (Tet) Inducible Systems at Addgene Cre-lox Guide...

  2. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...its native context, the tetracycline repressor (TetR) can bind to the tetracycline operator sequences (TetO...preventing transcription. In the presence of tetracycline (Tet), TetR preferentially binds Tet over the...repressible system, Gossen and Bujard created the tetracycline-controlled transactivator (tTA). They fused ...TRE)), allowing transcription to proceed. When tetracycline or one of its derivatives is added, it binds...information on Addgene Tet plasmids, see our Tetracycline resource page. Cumate Switch Massie et. al developed...control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci U S...Signals FLEx Vectors Resources at Addgene.org Tetracycline Collection ...

  3. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...by titration with different amounts of tetracycline. Tetracycline regulation is reversible (when the drug...with more elements of control. Tet promoters Tetracycline-dependent promoters are engineered by placing...constitutively silenced proteins. Addition of tetracycline or one of its analogs will induce these transcriptional...transcriptional level.     Fig. 2 Tetracycline on/off promoters and modes of action Check out...

  4. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...permeability Bactericidal 10-100 ug/mL Tetracycline tetracyclin Binds 30S ribosomal subunit; inhibits...in foil if a light-sensitive antibiotic like Tetracycline is used. Keep in mind that some E. coli strains...

  5. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...not recommended for toxic proteins BL21-A1 Tetracycline Arabinose-inducible expression of T7 RNAP; IPTG...T7RNAP-tetA General protein expression  BLR (DE3) Tetracycline RecA-deficient; best for plasmids with repetative...proteins Origami2 (DE3)** Streptomycin and Tetracycline Contains highly active thioredoxin reductase...

  6. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ... of XFPs in the original method, we used the Tetracycline trans-activator system to boost the expression...expression levels, and we named the system Tetbow (Tetracycline trans-activator Brainbow) (Sakaguchi et al.,...

  7. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ... streptomycin Tn10   Confers resistance to tetracycline Additionally, Table 2 provides a quick reference...galE15 galK16 rpsL(StrR) endA1 λ- XL1 Blue Tetracycline Blue/white screening and routine cloning, nalidixic...lacIq Δ(lacZ)M15] hsdR17(rK- mK+)  XL10 Gold Tetracycline and Chloramphenicol High competency cloning ...

  9. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...splice acceptor. TRE General expression mRNA Tetracycline response element promoter Inducible with Tetracyline...promoter with low basal activity and several tetracycline operators. Transcription can be turned on or...

  10. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...used inducible lentiviral vector system is the tetracycline (Tet)-regulated system. You can choose either...promoter in your transfer vector. In the absence of tetracycline or a derivative such as Doxycycline (Dox), the...

  13. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ... of shRNA in target cells by the addition of tetracycline/doxycycline to the grown media resulting in ...

  18. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...where gRNA expression is induced by anhydrous tetracycline. Three new anti-CRISPRs were identified that...

  19. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...with Cas9 on an integrative plasmid containing tetracycline repressor TetR and resistance selection marker...
Showing: 1 - 20 of 25 results