We narrowed to 7 results for: tk

1. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   .../10.7554/elife.38853 Hu C-D, Chinenov Y, Kerppola TK (2002) Visualization of Interactions among bZIP and...://doi.org/10.1016/s1097-2765(02)00496-3 Kerppola TK (2006) Design and implementation of bimolecular fluorescence.... https://doi.org/10.1038/nprot.2006.201 Kerppola TK (2013) Bimolecular Fluorescence Complementation (...

2. Adeno-associated Viruses (AAVs) for Genome Editing

   Type

   Blog Post

   Published

   March 27, 2018, 1:32 p.m.

   ...enrichment, whereas pAAV-TK-Acceptor has a conventional heterologous promoter-driven TK-neoR gene. To modify... AAV tagging vectors (pAAV-SEPT-Acceptor and pAAV-TK-Acceptor) so that they can be easily adapted to edit...

3. Plasmids 101: Knockout/Knock-In Plasmids

   Type

   Blog Post

   Published

   Dec. 1, 2016, 3:30 p.m.

   ... homology arms. The negative selection marker HSV-tk is used to select against random recombinants. ...selection marker like the HSV thymidine kinase (HSV-tk) is included just outside one of the homology arms...

4. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

   Type

   Blog Post

   Published

   Nov. 2, 2021, 1:15 p.m.

   ..., in a recent study by the Wickersham lab (Lavin, TK. 2020) it was determined that the optimal titer to...664–678. https://doi.org/10.1089/hum.2019.277 Lavin TK, Jin L, Lea NE, Wickersham IR (2020) Monosynaptic...

5. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   Published

   June 2, 2015, 6:06 p.m.

   ...Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22;54(4):698-710. doi: 10.1016...

6. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

   Type

   Blog Post

   Published

   Dec. 7, 2020, 2:53 p.m.

   ... A,  He C , Xu M, Zhou Z, Smock DC, Tang K, Owens TK, Krishnappa N, Sachdeva R, Deutschbauer AM, Banfield...

7. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

   Type

   Blog Post

   Published

   Sept. 28, 2020, noon

   ...doi.org/10.1093/nar/gkt520 Citorik RJ, Mimee M, Lu TK (2014) Sequence-specific antimicrobials using efficiently...