We narrowed to 8 results for: v5

1. Hot Plasmids: Fall 2024

   Type

   Blog Post

   Published

   Nov. 21, 2024, 1:30 p.m.

   ...through Addgene. Anti-V5 [IPI-SV5-Pk1] is a recombinant version of the original V5 tag mouse monoclonal...articles.  Here's what you'll find in this post: Anti-V5 and other new epitope tag antibodies from IPI New...Ashley Waldron We are excited to highlight the Anti-V5 tag antibody produced by our partners at the Institute...   Figure 1: Experimental validation for Anti-V5 [IPI-SV5-Pk1] in a western blot assay. Image reused...et al., 2024 under CC BY license.    Find Anti-V5 [IPI-SV5-Pk1] Antibody here!   Anderson, Z., Zhu...Moshinsky, D., Meijers, R. (2024). Western Blot for Anti-V5 [IPI-SV5-Pk1]. Addgene Report, https://doi.org/10.57733...

2. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

   Type

   Blog Post

   Published

   June 28, 2018, 3:01 p.m.

   ...successfully recombined the single-stranded donor DNA (V5 tag) were quantified by immunocytochemistry assays...the transfection, the entire plate was scanned for V5 tagging using an Operetta imaging system. Dewari ... ICC assay specificity. We generally include Sox2-V5 tagging as a positive control in our knock-in experiments...crRNA and/or try out an alternative tag (3xFLAG, Myc, V5, or HA). Tag-specific PCR genotyping can tell if ...

3. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

   Type

   Blog Post

   Published

   May 22, 2018, 1:38 p.m.

   ...Figure 1) from the two yeast species into the pYES2.1/V5-His yeast overexpression vector. This plasmid contains...the GAL1 promoter. The cloning procedure adds both V5 and His6 epitope tags to the C-termini of Met proteins...

4. Tag Your Favorite Yeast Genes with Ease

   Type

   Blog Post

   Published

   Nov. 19, 2013, 2:37 p.m.

   ...epitope tag (12xHis, 2xStrep, 3xFlag, Protein A, or V5), and selection marker (KanMX, HphMX, or His3MX)....

5. Fluorescent Tagging of Endogenous Genes with SapTrap

   Type

   Blog Post

   Published

   Oct. 12, 2017, 2:26 p.m.

   ...N-terminal tagging vectors (eg GFP, Flag, YFP, Strep, TEV-V5) with either blasticidin or puromycin selection. ...

6. Neuronal labeling with Spaghetti Monster

   Type

   Blog Post

   Published

   Aug. 14, 2018, 12:42 p.m.

   ...viral oncogene (myc), simian virus 5-derived epitope (V5), the synthetic peptide FLAG, the synthetic streptavidin-binding...

7. Antibodies 101: Immunoprecipitation

   Type

   Blog Post

   Published

   Dec. 7, 2021, 2:15 p.m.

   ...cases such as this, an epitope such as c-Myc, GFP or V5 can be used to tag the protein of interest on the...

8. Plasmids 101: Protein tags

   Type

   Blog Post

   Published

   Dec. 11, 2014, 4:26 p.m.

   ...HHSSGLVPRG  12 Solubililty Assists in proper folding V5 GKPIPNPLLGLDST   1.4 Affinity and Purification Good...