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We narrowed to 16 results for: vp64

Showing: 1 - 16 of 16 results

  1. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...lines.   dCas9-VP64 Description CRISPR activation can occur through fusing dCas9 with VP64, a strong transcriptional... fused with multiple copies of VP64. By having multiple copies of VP64 at each loci of interest, this ... significant improvements upon the initial dCas9-VP64 method, so there are multiple options to choose ...transcriptional activation domain. Guided by dCas9, VP64 recruits transcriptional machinery to specific sequences... which sequence is targeted. Performance dCas9-VP64 activation is generally thought of as the “first...been able to achieve much higher activation, dCas9-VP64 is great for experiments that requires modest gene...transcription. This is done through creating a dCas9/VP64 fusion protein engineered with aptamers that bind...

  2. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...specific genes using dCas9-VP64 activator fragments (dCas9(C)-FKBP-2xNLS-VP64 and dCas9(N)-FRB-NES). This...&62884; 62885 & 62886) Rapamycin-inducible dCas9-VP64 activator set (Addgene plasmids 62887 & 62888) ...protein domains CibN and Cry2 to inactive dCas9 and VP64, respectively. CibN and Cry2 form a heterodimer ...blue light, which will ultimately co-localize the VP64 transactivator with a dCas9 that has been targeted...activation when fused to dCas9. In the dCas9-SunTag-VP64 system, dCas9 is fused to a scaffold containing ... epitopes recognized by scFV antibodies fused to VP64 transcriptional activation domains. This system ...enabled the specific recruitment of multiple copies of VP64 to the sgRNA-targeted gene, resulting in increased...

  3. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...region. Cas9 activators include Cas9 fused to VP64, VP64 along with other SAM factors, and the Suntag ... to a single transcriptional activation domain - VP64.  Later generations of activators include the SunTag...generally the strongest activator, consists of a dCas9-VP64 fusion and a modified gRNA that is capable of interacting...

  4. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ... 2) CRY2FL-VP64, a fusion of full-length CRY2 and the transcriptional activator domain VP64. Using this...CRY2PHR) fused to a transcriptional activator domain (VP64 or p65). Upon expression in the cell, the dCas9-...fused BldD to the transcriptional activators p65 and VP64 which in turn transcribes N-Cas9. The N- and C-Cas9...

  6. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...Fusing dCas9 to a transcriptional activator like VP64 or VPR activates transcription, whereas dCas9-KRAB...increased transcriptional activation more than dCas9-VP64, especially when targeting distal enhancers. As ...

  8. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...able to activate expression by fusing dCas12f to VP64. Read the paper in Nature Biotechnology Find the...

  12. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...Cas9 activators, check out our blog post covering VP64, Suntag, SAM, and VPR. Worry less about off targets...

  15. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...Cas9, dCas9 (transcriptional repressor), and dCas9-VP64 (transcriptional activator)-containing plasmids....

  16. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...Gersbach pcDNA3.1-GoldenGate, pcDNA3.1-GoldenGate-VP64 Maria-Elena Torres-Padilla pTALYM3, pTALYM4 ...target genes (20-40x greater than standard dCas9-VP64 activators) when introduced into mammalian cells...
Showing: 1 - 16 of 16 results