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We narrowed to 24 results for: vp64

Showing: 1 - 20 of 24 results

  1. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ...lines.   dCas9-VP64 Description CRISPR activation can occur through fusing dCas9 with VP64, a strong transcriptional... fused with multiple copies of VP64. By having multiple copies of VP64 at each loci of interest, this ... significant improvements upon the initial dCas9-VP64 method, so there are multiple options to choose ...transcriptional activation domain. Guided by dCas9, VP64 recruits transcriptional machinery to specific sequences... which sequence is targeted. Performance dCas9-VP64 activation is generally thought of as the “first...been able to achieve much higher activation, dCas9-VP64 is great for experiments that requires modest gene...transcription. This is done through creating a dCas9/VP64 fusion protein engineered with aptamers that bind...

  2. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...specific genes using dCas9-VP64 activator fragments (dCas9(C)-FKBP-2xNLS-VP64 and dCas9(N)-FRB-NES). This...&62884; 62885 & 62886) Rapamycin-inducible dCas9-VP64 activator set (Addgene plasmids 62887 & 62888) ...protein domains CibN and Cry2 to inactive dCas9 and VP64, respectively. CibN and Cry2 form a heterodimer ...blue light, which will ultimately co-localize the VP64 transactivator with a dCas9 that has been targeted...activation when fused to dCas9. In the dCas9-SunTag-VP64 system, dCas9 is fused to a scaffold containing ... epitopes recognized by scFV antibodies fused to VP64 transcriptional activation domains. This system ...enabled the specific recruitment of multiple copies of VP64 to the sgRNA-targeted gene, resulting in increased...

  3. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...region. Cas9 activators include Cas9 fused to VP64, VP64 along with other SAM factors, and the Suntag ... to a single transcriptional activation domain - VP64.  Later generations of activators include the SunTag...generally the strongest activator, consists of a dCas9-VP64 fusion and a modified gRNA that is capable of interacting...

  4. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ... 2) CRY2FL-VP64, a fusion of full-length CRY2 and the transcriptional activator domain VP64. Using this...CRY2PHR) fused to a transcriptional activator domain (VP64 or p65). Upon expression in the cell, the dCas9-...fused BldD to the transcriptional activators p65 and VP64 which in turn transcribes N-Cas9. The N- and C-Cas9...

  5. Lentiviral Prep Service

    Type
    Collection
    ...Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A GFP Zhang... dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64 with ...Human CRISPR inhibition library Dolcetto in XPR_050 (VP64) Set A Human CRISPR inhibition library in backbone...Human CRISPR inhibition library Dolcetto in XPR_050 (VP64) Set B Human CRISPR inhibition library in backbone...2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang Pooled Barcoding Libraries ...

  7. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...Fusing dCas9 to a transcriptional activator like VP64 or VPR activates transcription, whereas dCas9-KRAB...increased transcriptional activation more than dCas9-VP64, especially when targeting distal enhancers. As ...

  8. TALEN Plasmids and Kits

    Type
    Collection
    ...NLS and HA tags. Plasmid 47389 also contains the VP64 domain (four repeats of the minimal activation domain...VP16) at the C terminus. 47389 pcDNA3.1-GoldenGate-VP64 Golden Gate TALEN 2.0 47874 pTALYM3 Maria-Elena ...

  10. Zhang Lab CRISPR Page

    Type
    Collection
    ...three components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating two MS2 RNA aptamers...transient transfection : 61422 : Expresses dCAS9-VP64 activator with 2A GFP 61423 : Expresses the MS2-...production : 61425 : lentiviral vector encoding dCAS9-VP64 with 2A Blast resistance marker 61426 : lentiviral...

  19. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Gersbach pcDNA3.1-GoldenGate, pcDNA3.1-GoldenGate-VP64 Maria-Elena Torres-Padilla pTALYM3, pTALYM4 ...target genes (20-40x greater than standard dCas9-VP64 activators) when introduced into mammalian cells...

  20. Plan Your Experiment

    Type
    Collection
    ...fusion with transcriptional activator (such as dCas9-VP64) Many different activator systems exist, including...
Showing: 1 - 20 of 24 results