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We narrowed to 33 results for: Kars;

Showing: 1 - 20 of 33 results

  1. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ... Depositor Collections Bikard Lab - CRISPR Repression Collection Bikard Lab CRISPR Repression Plasmids...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently...Protocols gRNA Design Tools CRISPR Blog Posts The David Bikard Lab at the Institute Pasteur has deposited a collection...article: Vigouroux, A., Oldewurtel, E., Cui, L., Bikard, D., & Van Teeffelen, S. (2018). Tuning dCas9’s...

  2. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Optogenetics Karl Deisseroth AV-1-26966P 26966-AAV1 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-1-26968P 26968-AAV1 pAAV-Ef1a-DIO ChETA-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-1-26971P 26971-AAV1 pAAV-CaMKIIa-eNpHR 3.0-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-1-26975 26975-AAV1 pAAV-CaMKIIa-hChR2(H134R)-mCherry Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-5-26966P 26966-AAV5 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-5-26969P 26969-AAV5 pAAV-CaMKIIa-hChR2(H134R)-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-5-26973P 26973-AAV5 pAAV-hSyn-hChR2(H134R)-EYFP Optogenetics Karl Deisseroth...

  3. Optogenetics AAV Preps

    Type
    Collection
    ...dependent 8 Karl Deisseroth 137144 pAAV-nEF-Coff/Fon-ChR2-mCherry nEF ChR2 mCherry Flp dependent 8 Karl Deisseroth...dependent 8 Karl Deisseroth 26968 pAAV-Ef1a-DIO ChETA-EYFP EF1a ChETA EYFP Cre dependent 1, 5, 9 Karl Deisseroth...rg* Karl Deisseroth 26971 pAAV-CaMKIIa-eNpHR 3.0-EYFP CaMKII eNpHR 3.0 EYFP Constitutive 1, 9 Karl Deisseroth...Constitutive 2, 5 Karl Deisseroth 137151 pAAV-nEF-NpHR3.3-EYFP nEF NpHR 3.3 EYFP Constitutive 8 Karl Deisseroth...dependent 8 Karl Deisseroth 137154 pAAV-nEF-Coff/Fon-NpHR3.3-EYFP nEF NpHR 3.3 EYFP Flp dependent 8 Karl Deisseroth...dependent 8 Karl Deisseroth 137156 pAAV-nEF-Con/Foff 2.0-iC++-EYFP nEF iC++ EYFP Cre dependent 8 Karl Deisseroth...pACAGW-ChR2-Venus-AAV CAG ChR2 Venus Constitutive 1, 9 Karel Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell-3 Dlx...

  4. Control AAV Preps

    Type
    Collection
    ...PHP.eB Karl Deisseroth 114472 pAAV-hSyn-mCherry hSyn mCherry Constitutive 1, 2, 5, 8, 9, rg* Karl Deisseroth...Constitutive 8 Karl Deisseroth 131001 pAAV-hSyn-mScarlet Syn mScarlet Constitutive 1, 8 Karl Deisseroth 135630...dependent 9 Karl Deisseroth 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP Cre dependent 1, 2, 5, 9, rg* Karl Deisseroth...dependent 8 Karl Deisseroth 137133 pAAV-Ef1a-Con/Foff 2.0-mCherry EF1a mCherry Cre dependent 8 Karl Deisseroth...dependent 8 Karl Deisseroth 137162 pAAV-Ef1a-Con/Foff 2.0-EYFP EF1a EYFP Cre dependent 8 Karl Deisseroth..., rg* Karl Deisseroth 55641 pAAV-Ef1a-fDIO EYFP EF1a EYFP Flp dependent 1, 2, 5, 8, 9, rg* Karl Deisseroth...dependent 8 Karl Deisseroth 137134 pAAV-Ef1a-Coff/Fon-mCherry EF1a mCherry Flp dependent 8 Karl Deisseroth...

  5. Retrograde AAV viral preps

    Type
    Collection
    ...mCherry Control Karl Deisseroth 114469 pAAV-CaMKIIa-mCherry CamKII mCherry Control Karl Deisseroth 50459...Cre-dependent Control Karl Deisseroth 55641 pAAV-Ef1a-fDIO EYFP EF1a EYFP, Flp-dependent Control Karl Deisseroth ...Control Karl Deisseroth 114471 pAAV-Ef1a-fDIO mCherry EF1a mCherry, Flp-dependent Control Karl Deisseroth... Recombinases Karl Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases Karl Deisseroth 55637...Recombinases Karl Deisseroth 55638 pAAV-EF1a-vCre EF1a VCre expression Recombinases Karl Deisseroth 87306...Optogenetics Karl Deisseroth 26973 pAAV-hSyn-hChR2(H134R)-EYFP Syn Activator Optogenetics Karl Deisseroth...Optogenetics Karel Svoboda 26975 pAAV-CaMKIIa-hChR2(H134R)-mCherry CamKII Activator Optogenetics Karl Deisseroth...

  6. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...An overview of CRISPR's development from a prokaryotic immune system to a valuable genome engineering...revolution shows no signs of slowing down. This prokaryotic immune system is especially amenable to genome...offering flexibility and easy multiplexing/scaling. Prokaryotes have long utilized CRISPR as a powerful defensive...elucidated until 2007. Scientists hypothesized that prokaryotes used CRISPR as part of an adaptive immune system...PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types and 16...system seems like a winning strategy to ensure prokaryotic survival, but it is not invincible. Bondy-Denomy...provides acquired resistance against viruses in prokaryotes. Science . 315(5819):1709-12. PMID: 17379808 ...

  7. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...OPTN His T7 ALS Jon Ashwell 23376 pDONR223-PRKAR1B PRKAR1B Dementia and/or parkinsonism William Hahn 23452...Parkinson's, FTD Karen Ashe 46905 pRK5-EGFP-Tau AP MAPT GFP CMV Parkinson's, FTD Karen Ashe 46906 pRK5-...Parkinson's, FTD Karen Ashe 46907 pRK5-EGFP-Tau E14 MAPT GFP CMV Parkinson's, FTD Karen Ashe 46908 pRK5...Parkinson's, FTD Karen Ashe 46909 pRK5-EGFP-Tau E14 P301L MAPT GFP CMV Parkinson's, FTD Karen Ashe 46924 YFP-Parkin...-Spatz disease David Root 76838 PRKAR1B gRNA (BRDN0001146606) PRKAR1B hU6 Dementia and/or parkinsonism...parkinsonism David Root 76839 PRKAR1B gRNA (BRDN0001144765) PRKAR1B hU6 Dementia and/or parkinsonism David Root ...Root 76840 PRKAR1B gRNA (BRDN0001147089) PRKAR1B hU6 Dementia and/or parkinsonism David Root 76898 PRKCG ...

  8. Recombinases AAV Preps

    Type
    Collection
    ... a fusion tag) 8, rg* Karl Deisseroth 55636 pAAV-EF1a-Cre EF1a none 11, rg* Karl Deisseroth 121675 pAAV-EF1a-fDIO-Cre... fusion tag) 1, rg* Karl Deisseroth 55637 pAAV-EF1a-Flpo EF1a none 1, 2, rg* Karl Deisseroth 87306 AAV...Viral Tools 183535 pAAV-CaMKIIa-Flpo CaMKIIa none 8 Karl Deisseroth 236288 pAAV.TBG.PI.FLPo.rBG TBG none ...Serotype(s) PI 55638 pAAV-EF1a-vCre EF1a none 8, rg* Karl Deisseroth *AAVrg = retrograde serotype, produced...produced with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory...

  9. CRISPR Guide

    Type
    Collection
    ...bacterial species, but newer versions engineered for eukaryotic cells are available and further improvements ...Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex is recruited to target...25122746 Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. ... Science , 339 (6121), 823–826. PMID: 23287722 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa,... Kriz, A. J., Zetsche, B., Shalem, O., Wu, X., Makarova, K. S., Koonin, E. V., Sharp, P. A., & Zhang, .... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P., Chen, C., Nelson, J. W., Newby..., Herreno-Pachón, A., Benincore-Flórez, E., Karunathilaka, A., & Tomatsu, S. (2024). Current strategies...

  10. Bacterial Expression Systems

    Type
    Collection
    ...luminescence (lux operon) Gram-negative bacteria Attila Karsi 14080 pAKlux2 Promoter activity Luminescence (lux...lux operon) Gram-negative bacteria Attila Karsi 14076 pAKgfp1 Promoter activity Fluorescence (GFPmut3a...GFPmut3a) Gram-negative bacteria Attila Karsi 18855 FLIParaF.Ec-250n Arabinose FRET fluorescence (CFP and Venus...Fluorescence (GFPmut2) Escherichia coli Rahul Sarpeshkar 111614 pCdrA-gfpC Cyclic di-GMP Fluorescence ...

  11. Tetracycline Inducible Expression

    Type
    Collection
    ... and reversible control of gene expression in eukaryotes. To avoid secondary effects from repurposing ... Tet-On vector with CMV promoter Tet-On 3G rtTA Oskar Laur 96963 pCAG-TetON-3G Mammalian expression of...2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-...(Link opens in a new window) External Resources Takara: Tet-inducible Systems Overview (Link opens in ...

  12. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ... 33,170 Bikard Lab E. coli Genome-wide Inhibition Library v1 115927 Inhibition E. coli Bikard N/A 19 92,919...92,919 Bikard Lab EcoWG1: E. coli Genome-wide Inhibition Library v2 131625 Inhibition E. coli Bikard N/A ...

  13. Chemogenetics AAV Preps

    Type
    Collection
    ...Gi) - Inhibition mCherry Cre and Flp-dependent 8 Karl Deisseroth 177673 pAAV-nEF-Con/Foff DREADD Gi-mCherry...Gi-mCherry rM4D(Gi) - Inhibition mCherry Cre-dependent 8 Karl Deisseroth 135635 pAAV-S5E2-Gq-P2A-dTomato hM3D(...produced with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory...

  14. Immunology Research Plasmids and Resources

    Type
    Collection
    ... CKR-1, CMKAR1, CXCR1, IL8R1, IL8RBA IL8RB interleukin 8 receptor, beta CD182, CDw128b, CMKAR2, CXCR2,... (C-X-C motif) receptor 3 CD182, CD183, CKR-L2, CMKAR3, GPR9, IP10-R, Mig-R, MigR CXCR4 chemokine (C-X-C... protein tyrosine kinase binding protein DAP12, KARAP, PLOSL ULBP1 UL16 binding protein 1 RAET1I ULBP2...Smith B, Schaefer H, Chen J, Hu Z, Zalocusky KA, Shankar RD, Shen-Orr SS, Thomson E, Wiser J, Butte AJ. ...

  15. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...PXL 75349 Mammalian U6 yes, activate S. pyogenes Luikart pRubiG-T2A-Cas9 75348 Mammalian/Retroviral from...from pXL yes, activate S. pyogenes EGFP Luikart pRubiC-T2A-Cas9 75347 Mammalian/Retroviral from pXL yes,...yes, activate S. pyogenes mCherry Luikart pDECKO_mCherry 78534 Mammalian hU6 none S. pyogenes Puro, mCherry...

  16. mTOR Pathway

    Type
    Collection
    ...provided below. mTORC1 Complex Symbol Name 4E-BP Eukaryotic translation initiation factor 4E, binding protein...protein 1 Cyclin D1 Also known as CCND1 eIF-4E Eukaryotic translation initiation factor 4E GBL/mLST8 MTOR...

  17. Viral Production

    Type
    Collection
    ...detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). ...Mycoplasma The 293T cell line was obtained from Takara, and is routinely tested for mycoplasma contamination...

  18. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... tagged recombinant protein pDest-Sumo - For prokaryotic expression of target protein as N-terminal fusion...selection Puromycin Mammalian pcDNA3.1-(-)-Pur - Eukaryotic expression vector Tet-pLKO-puro - ...

  19. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...well-characterized proteins. General Structures Eukaryotic cells contain a variety of organelles and subcellular...Rubinsztein 11546 EGFP-LC3 Autophagosome LC3 EGFP Karla Kirkegaard 61205 pCMB-AUTr Autophagosome LC3(ATG8...

  20. Brain Initiative Collection

    Type
    Collection
    ... PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Cre-dependent recombination...
Showing: 1 - 20 of 33 results