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Showing: 1 - 20 of 51 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Empty backbones are plasmids containing...Lentiviral sgRNA cloning backbone pLKO.1-blast - 3rd gen lentiviral backbone for cloning and expression...Mammalian lenti sgRNA(MS2)_zeo backbone - 3rd gen lenti sgRNA cloning backbone with MS2 loops at tetraloop...A selection of Addgene's empty vector backbones curated by species, epitope tags/fusion proteins, function...various conditions. When choosing what plasmid backbone to use, you have many elements to consider. Here...guide to a selection of Addgene's empty vector backbones. For the most part, we will assume that you want... Host Relevant Promoters Representative Empty Backbones Mammalian CMV, SV40, EF1a, CAG, Ubc Transient ...

  2. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Fluorescent Proteins Empty Backbones Fluorescent Proteins: Empty Backbones Blue/UV Cyan Green Yellow Orange... present. Looking for more empty backbones? Visit our Empty Backbones page or search our complete collection...Explore empty plasmid backbones with different fluorescent tags to create fusion proteins with your gene...list many popular fluorescent proteins and empty backbones for expression and cloning of fusion proteins,...Additional Resources Addgene Collections Empty Backbones Michael Davidson Fluorescent Protein Collection...

  3. Lentiviral Prep Service

    Type
    Collection
    ...Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation library in backbone XPR_502 ...Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing...sgRNAs along with 1000 non-targeting controls. This backbone does not contain SpCas9. 76,441 unique sgRNAs ...library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing...sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs...genes and containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting...VP64) Set A Human CRISPR inhibition library in backbone pXPR_050 inhibiting 18,901 genes and containing...

  4. Zhang Lab CRISPR Page

    Type
    Collection
    ...60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone cloning information:...helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop - Contains...resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and...luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase Vectors for...LacZ, plus Cre recombinase 60229 : sgRNA cloning backbone with Cre recombinase 60230 : sgRNA targeted to...adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described ...control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR This plasmid ...

  5. Immunology Research Plasmids and Resources

    Type
    Collection
    ...BMP5 bone morphogenetic protein 5 MGC34244 BMP6 bone morphogenetic protein 6 VGR, VGR1 BMP7 bone morphogenetic... MGC34632 BMP1 bone morphogenetic protein 1 FLJ44432, PCOLC, PCP, TLD, pCP-2 BMP10 bone morphogenetic ...protein 10 MGC126783 BMP15 bone morphogenetic protein 15 GDF9B, ODG2, POF4 BMP2 bone morphogenetic protein ...protein 2 BMP2A BMP3 bone morphogenetic protein 3 BMP-3A BMP4 bone morphogenetic protein 4 BMP2B, BMP2B1, MCOPS6...protein 7 OP-1 BMP8A bone morphogenetic protein 8a FLJ14351, FLJ45264 BMP8B bone morphogenetic protein...BMPR1A bone morphogenetic protein receptor, type IA 10q23del, ACVRLK3, ALK3, CD292, SKR5 BMPR1B bone morphogenetic...

  6. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Feature 101137 pSNAP-tag (T7) Vector SNAP-tag Empty backbone for bacterial expression under T7 control 101135...101135 pSNAP-tag (m) Vector SNAP-tag Empty backbone for stable and transient mammalian expression 101133...localization 101136 pCLIP-tag (m) Vector CLIP-tag Empty backbone for stable and transient mammalian expression ...localization 101127 pMCP-tag (m) Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag...pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient mammalian expression 101132 pMCP-GPI...Mootz HD, Bergendahl V, Marahiel MA.1998. Peptide bond formation in nonribosomal peptide biosynthesis. ...

  7. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ... Vincent Pieribone AV-1-36857P 100037-AAV1 pAAV.hSynap.ArcLightD.WPRE.SV40 Vincent Pieribone AV-1-51473...mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-50943 50943-...mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68717 68717-...pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68719 68719-...pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68720 68720-...Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-PV2723 98929...pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-PV2521 105551...

  8. Luciferase Plasmid Collection

    Type
    Collection
    ...Luciferase blog post . Empty Backbones Browse or search for popular empty backbone plasmids into which you ... Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs Other...Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α ...pMKV060 Firefly AtUbi10 BeYDV replicon on T-DNA backbone expressing firefly luc for monitoring plant transfection...pMKV057 Firefly AtUbi10 BeYDV replicon on T-DNA backbone expressing WUS2 and IPT, with firefly luc for ...

  9. Tetracycline Inducible Expression

    Type
    Collection
    ...tet-inducible vectors. Tetracycline-inducible Backbones Find backbones or constructs to use for cloning to control...Browse the tables below to find popular empty backbones, expression vectors for transactivators, and vectors...inducible RNAi with miR-E (miR-30 variant)-based backbone rtTA-Advanced TRE3G Johannes Zuber 60495 pSBtet-GP...Tight TRE David Sabatini 19407 pTREtight2 Empty backbone for Tet-controlled expression (On or Off) None...transactivator under the c-Fos promoter Tet-On 3G rtTA Bong-Kiun Kaang 20342 FUW-M2rtTA Lentiviral Tet-On vector...

  10. Fluorescent Proteins: FRET

    Type
    Collection
    ...properties. Where available, we have listed cloning backbones and control plasmids. Donor Acceptor λ Dex QY ...Amber (mVenus Y67C) FRET standards and cloning backbones Koushik et al. (2006) Cerulean, Venus, and VenusY67C...FRET Positive and negative controls and cloning backbones for mTurquoise2-mVenus(L68V) Goedhart et al. (...93% Positive and negative controls and cloning backbones for various FRET pairs with mTurquoise2 Mastop...mTurquoise2 Positive and negative controls and cloning backbones for red, yellow, and turquoise FRET pairs Gadella...

  11. CRISPR Guide

    Type
    Collection
    ...been created in AAV backbones for in vivo experiments and in a retroviral backbone for delivery to cells... gRNA and Cas9 protein, which will form a ribonucleoprotein complex through interactions between the gRNA...disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 — increase Cas9 proofreading and discrimination...endonuclease. Nature , 513 (7519), 569–573. PMID: 25079318 Bondy-Denomy, J., Pawluk, A., Maxwell, K. L., & Davidson...system. Nature , 493 (7432), 429–432. PMID: 23242138 Bondy-Denomy, J., Garcia, B., Strum, S., Du, M., Rollins... Maddalo, D., Manchado, E., Concepcion, C. P., Bonetti, C., Vidigal, J. A., Han, Y., Ogrodowski, P., Crippa...Marino, N. D., Pinilla-Redondo, R., Csörgő, B., & Bondy-Denomy, J. (2020). Anti-CRISPR protein applications...

  12. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...fluorescent protein fusion constructs and cloning backbones from the Michael Davidson Lab....Davidson Fluorescent Protein Collection Empty Backbones Blue Cyan Green mEmerald Yellow Orange Red Photoactivatable...Davidson lab has contributed the corresponding empty backbone with different fluorescent tags for you to create...images, tutorials, protocols, and more. Empty Backbones Plasmid Description Industry Return to top Blue...

  13. Biosensor AAV Preps

    Type
    Collection
    ...GSG-P2A-GCaMP6f-WPRE-pA Syn GCaMP6f mRuby2 Constitutive 1 Tobias Bonhoeffer , Mark Huebener , Tobias Rose 51083 AAV-EF1a-...-pA CAG GCaMP6f mRuby2 Cre dependent 1 Tobias Bonhoeffer , Mark Huebener , Tobias Rose 83899 pAAV-mDlx-GCaMP6f-Fishell...GSG-P2A-GCaMP6s-WPRE-pA Syn GCaMP6s mRuby2 Constitutive 1 Tobias Bonhoeffer , Mark Huebener , Tobias Rose 51082 AAV-EF1a-...-pA CAG GCaMP6s mRuby2 Cre dependent 1 Tobias Bonhoeffer , Mark Huebener , Tobias Rose 68720 pAAV-hSyn1...-pA Syn GCaMP6s mRuby2 Cre dependent 1 Tobias Bonhoeffer , Mark Huebener , Tobias Rose 100842 pAAV.CAG.Flex.GCaMP6s.WPRE.SV40...

  14. TALEN Plasmids and Kits

    Type
    Collection
    ...-DD and pCS2TALE3-RR are next generation TALEN backbone vectors designed for use with the Voytas Golden...introduced to the N- and C-terminus of the pTAL3 TALEN backbone, which were initially published by Sangamo BioSciences...mammalian cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (...with the Voytas Lab Golden Gate TALEN kit. This backbone is suitable for the TALEN-based generation of ...

  15. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...to top Recombinase-Dependent Empty Backbones Find an empty backbone to regulate your gene of interest....Recombinase Plasmids Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries/Kits Site-specific... other recombinases , as well as useful empty backbones or reporters . Experimental Considerations Recombinase...

  16. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...The plasmids listed here are provided as empty backbones that utilize Golden Gate cloning to insert the...desired guide RNA sequence by digestion of the backbone with BbsI followed by a quick ligation of annealed...and Sequence Derivative Sources Original pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2 ( Addgene...

  17. Zebrafish Plasmid Collection

    Type
    Collection
    ...This includes plasmids for genome editing, empty backbones, fluorescent proteins, reporters, biosensors, ...selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR resources...pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS), bearing...

  18. Lentivirus Plasmids

    Type
    Collection
    ...Modular CD28-CD3z CAR backbone. See Bloemberg et al. (2020) for additional CAR backbones. Scott McComb 145025...selection. See Root Lab pLEX and pLIX Gateway backbones for additional variants. David Root 85140 pLV-...

  19. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...prokaryotic survival, but it is not invincible. Bondy-Denomy et al. identified the first set of anti-CRISPR...prokaryotes. Science . 315(5819):1709-12. PMID: 17379808 Bondy-Denomy J, Garcia B, Strum S, Du M, Rollins MF, Hidalgo-Reyes...proteins. Nature . 526(7571):136-9. PMID: 26416740 Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR. 2013...

  20. Fluorescent Protein Guide

    Type
    Collection
    ...Fluorescent Protein Plasmids by Category Empty Backbones Study your protein of interest by fusing it to...fluorescent proteins as well as hundreds of empty backbones. FRET Find FRET pairs and standards to study protein-protein...
Showing: 1 - 20 of 51 results