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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 78 results for: sequence

Showing: 1 - 20 of 78 results

  1. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...given gRNA sequence exactly match your genomic target? Variation between a given gRNA sequence and your .... aureus etc.) was this gRNA sequence designed for? A given gRNA sequence may only be compatible with ...upstream of a 5' NGG 3' PAM sequence. Which CRISPR application is this gRNA sequence compatible with? CRISPR...determine if your cell type contains the target sequence before using any of these gRNA plasmids and review...target may reduce the gRNA activity. The variety in sequence decreases the gRNA activity and increases off-...CRISPR knockout experiments use targeting sequences within exons, whereas CRISPR activation or repression...published and unpublished plasmids containing gRNA sequences designed to be used with any CRISPR nuclease and...

  2. CRISPR References and Information

    Type
    Collection
    ...meningitidis Cas9, or Cas12a from your input sequence or list of sequences. Developed by the George Church Lab ...new window) Identifies gRNA target sequences from an input sequence and checks for off-target binding....window) Identifies candidate gRNA target sequences in an input sequence, which can be an accession number, ..., genomic location, pasted nucleotide sequence, or a sequence text file you upload. Currently supports...program that identifies gRNA target sequences from an input sequence, checks for off-target binding and...window) Identification of gRNA target sequences from an input sequence and checks for off-target binding....

  3. Plan Your Experiment

    Type
    Collection
    ...perfect world, your gRNA sequence would be an exact complement to your target sequence with no homologous sites... the desired target sequence, or on-target activity. Two gRNA targeting sequences with 100% homology to... selected target sequence. Additionally, any mismatches between a given gRNA sequence and your genomic...customization Usually paired with a sequencing method Can’t provide specific sequence information alone Digital ...rare edits Many options like RNA-sequencing, Perturb-seq, deep sequencing, etc. qPCR Knockouts Activation...and the guide RNA (gRNA), the design of the gRNA sequence, and finally how to validate your genetic edit...within a very narrow window proximal to the PAM sequence Base Edit Prime Edit Generate targeted insertions...

  4. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...target have high sequence identity to the spacer sequence and contain a PAM sequence just downstream of...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR...they have recognized and cleaved the target RNA sequence, they adopt an enzymatically active state and ... of the sequence to be cleaved. This non-specific cleavage is thought to activate programmed cell death...

  5. CRISPR Guide

    Type
    Collection
    ... be any ~20 nucleotide sequence, provided it meets two conditions: The sequence is unique compared to ...(PAM) The PAM sequence (NGG) serves as a binding signal for Cas9, but the exact sequence depends on which... target, the seed sequence (8–10 bases at the 3′ end of the gRNA targeting sequence) will begin to anneal...matters. Mismatches between the target sequence in the 3′ seed sequence inhibit target cleavage, while mismatches...determined by both the gRNA sequence and the Cas9 enzyme. Ideally, a gRNA targeting sequence will have perfect ...require PAM sequences in very close proximity to the region to be edited, making the PAM sequence (NGG) limiting...target sequence in your donor DNA with a silent mutation that does not change the amino acid sequence. HDR...

  6. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...the guide sequences consist of a 20-mer (“protospacer sequence”) upstream of an “NGG” sequence (“protospacer...reverse complement of each guide sequence. Example reverse complement sequences of the Pim1 sgRNA from Table...representative sequencing primer; other flanking primers may be utilized. Choose a sequence-verified colony...tools 1 . Use these tools to help identify guide sequences that minimize identical genomic matches or near-matches.... NOTE: In this example, sgRNA-A’s protospacer sequence and PAM happen to fall on the bottom (Crick) strand... strand while sgRNA-B’s protospacer sequence and PAM fall on the top (Watson) strand ( Figure 2A ). However... independent of orientation of the protospacer sequence/PAM relative to the top or bottom strand. Determine...

  7. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...target have high sequence identity to the spacer sequence and contain a PAM sequence just downstream of...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...promoter/enhancer or the beginning of the coding sequence. If the plasmid you’re using does not also express...they have recognized and cleaved the target RNA sequence, they adopt an enzymatically active state and ... of the sequence to be cleaved. This non-specific cleavage is thought to activate programmed cell death...immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence to direct dCas9 to a specific locus, avoiding ...

  8. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...additional 5' clamping sequence such as GTTTCTTC). Note that all prefix/suffix sequences are given for the ...Assembly standards define standard prefix and suffix sequences that make it easy to combine components for synthetic...sites which preserve a standard prefix and suffix sequence at either end of the assembly, even as more parts...registries, including the specific prefix and suffix sequences for each (these can be built into PCR primers ...prohibited sites and must be omitted from the coding sequence of the part and backbone. For some standards, ...Thr-Gly; Use N-parts prefix to preserve N-term sequence. Prohibited Restriction Sites: EcoRI, XbaI, NgoMIV...

  9. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...Design your gRNA sequence to direct the dCas9-FP fusion to a specific genomic sequence - potential target...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...promoter/enhancer or the beginning of the coding sequence. If the plasmid you’re using does not also express...dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system... For both enCHIP and CAPTURE, design your gRNA sequence to direct dCas9 to a specific locus, avoiding ...regions. Fluorophores may also be fused to gRNA sequences to produce fluorescence at a target region, as...

  10. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...-MeCP2 fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion for gene activation...express dCas9-VPR for activation. Usage Guide sequences can be designed using Benchling or using similar...Golden Gate cloning to insert the desired guide RNA sequence by digestion of the backbone with BbsI followed...phosphorylated oligonucleotides containing the guide target sequence: Up to two guides can be expressed from the same... Dual Guide Protocol (PDF, 990 KB) Plasmid and Sequence Derivative Sources Original pX458 backbone ( Addgene...

  11. Validated gRNA Sequences

    Type
    Collection
    ...given gRNA sequence exactly match your genomic target? Variation between a given gRNA sequence and your .... aureus etc.) was this gRNA sequence designed for? A given gRNA sequence may only be compatible with ...upstream of a 5' NGG 3' PAM sequence). Which CRISPR application is this gRNA sequence compatible with? CRISPR...accomplish. Validated gRNA Sequence Datatable Target Gene Target Species Target Sequence Plasmid ID Application...and outcomes. Submit Validated gRNA Sequences Validated gRNA sequences can be added to this table from any... the sequences, and email the file to [email protected] with the subject heading "gRNA sequence spreadsheet...Datatable of validated gRNA sequences. Genome...

  12. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Export Sequence mScarlet-I Dorus Gadella 85061 pmScarlet-H_NES_C1 Non-nucleus Nuclear Export Sequence mScarlet-H...Palmitoylation sequence from GAP43 EGFP Connie Cepko 61099 Lck-GFP Membrane Palmitoylation sequence from Lck... KDEL sequence mNeonGreen Dorus Gadella 137805 ER-mScarletI Endoplasmic Reticulum KDEL sequence mScarlet-I...2XG4S-mCer3 Mitochondria COX IV-derived (1-22 aa) import sequence and COX VIII signal peptide dL5 FAP; mCerulean3...mCherry-Mito-7 Mitochondria Mitochondrial targeting sequence(COX8A) mCherry* Michael Davidson 37354 pQC NLS...36206 pmTurquoise2-NES Non-nucleus Nuclear Export Sequence mTurquoise2 Dorus Gadella 85062 pmScarlet-i_NES_C1...85060 pmScarlet_NES_C1 Non-nucleus Nuclear Export Sequence mScarlet Dorus Gadella 37351 pQC membrane TdTomato...

  13. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... DNA sequence complementary to the crRNA. In Type II CRISPR systems, a potential target sequence is only...Regularly Interspaced Short Palindromic Repeat) sequences were initially discovered in the E. coli genome...loci to generate CRISPR RNAs (crRNAs). Through sequence homology, these crRNAs guide a Cas nuclease to...material, which must also contain a species-specific sequence known as a protospacer adjacent motif (PAM). The...CRISPR array contains many unique protospacer sequences that have homology to foreign DNA. Protospacers...Protospacers are separated by short palindromic repeat sequences. (1) The CRISPR array is transcribed to make the...genomes, are highly diverse, with varying PAM sequences and number/types of Cas proteins. Makarova et ...

  14. Zhang Lab CRISPR Page

    Type
    Collection
    ...target site sequence (20bp) and need to be followed on the 3' end by a 3bp NGG PAM sequence. We have found...target site sequence (30bp) and need to be followed on the 3' end by a 3bp NGG PAM sequence. 3. SpCas9 ...target site sequence (21-22bp) and need to be followed on the 3' end by NGRRT or NGRRN PAM sequences. We have... - Contains BbsI sites for insertion of guide sequences Addgene plasmids #61425 - #61427 are intended .... Contains BsmBI sites for insertion of guide sequences Zhang Lab SAM Cloning Protocol 321.5 KB Return...phosphorylated oligos designed based on the target site sequence (20bp). CRISPR-Cas9 Cre expression vectors for...phosphorylated oligos designed based on the target site sequence (20bp). #60230 - AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre...

  15. All Antibodies

    Type
    Collection
    ...details including the protein and plasmid sequence, the antigen sequence, and cross-species reactivity. Reactivity... trusted partner labs or is predicted based on sequence homology with the antigen. Addgene supplies a ...

  16. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...DNA prep, the plasmid sequence has been validated by next generation sequencing. The DNA concentration...Quality Control Plasmid verified by next generation sequencing Plasmids Available as Cloning Grade DNA ID Plasmid...

  17. CRISPR Plasmids - Yeast

    Type
    Collection
    ...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...promoter/enhancer or the beginning of the coding sequence. If the plasmid you’re using does not also express...immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence to direct dCas9 to a specific locus, avoiding ...

  18. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Use the target site sequence for your recombinase (see Table 1) to Search by Sequence to find other Cre-...recombinases and tyrosine recombinases, based on sequence similarity and the amino acid residue used in ...DNA strand (in cis) in the same direction, the sequence between the sites is excised as a circular piece...recombinase systems. Enzyme Target Site Canonical Sequence Structure (Length) Common Variants Cre loxP ATAACTTCGTATAgcatacatTATACGAAGTTAT...target sites. Codon optimization: Modifying the DNA sequence of the recombinase to align with your organism's...

  19. TALEN Plasmids and Kits

    Type
    Collection
    ...transcription initiation when targeted to DNA sequences within core promoter regions. pTAL5-BB contains... place of pTAL1, 2, 3, or 4. For both plasmids sequence positions 1214–2210 of pTAL3 were cloned into ...visualize subcellular positioning of target DNA sequences in living cells (TALE-mediated Genome Visualization...white-screening in E.coli , (iii) CAG promoter and Kozak sequence to drive efficient TALEN expression in mammalian...vectors for use in labeling specific repetitive DNA sequences in human chromosomes Do you have suggestions for...

  20. Viral Production

    Type
    Collection
    ...please contact us . Full sequencing of the Viral Genome Next-generation sequencing is performed on viral ...genes, and a plasmid encoding adenoviral helper sequences. AAV preparations are purified by iodixanol gradient...genomes isolated from the final AAV preparation. Sequencing results are analyzed to confirm the identity ...
Showing: 1 - 20 of 78 results