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We narrowed to 97 results for: REN

Showing: 11 - 20 of 97 results

  1. Viral Vectors

    Type
    Collection
    ...I Use? Use this guide to learn more about the different types of viruses that are commonly used in research... cancer therapy because of their ability to preferentially infect and kill cancer cells. Adenoviruses ...(through standard cloning methods). There are different types of viruses that are commonly used for research...research, each of which exhibit different properties, and thus, are suited for specific research goals...in-vivo studies because of its low immunogenicity. Different types of viruses also vary in the composition ... host. Use this guide to learn more about the different types of viruses that are commonly used in research...provides a brief overview of the properties of different types of viruses. More detailed information can...

  2. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...Deletion Isolate gDNA from parental and bulk sorted cells by resuspending parental and bulk cell pellets in... CRISPR References & Information Genomic Deletions in Mammalian ...elements in mammalian cell lines. Protocol For referenced Tables and Figures, please access the manuscript... cells are suspension cells, instructions for adherent cells have also been included. Ensure there are.... NOTE: This protocol is for suspension cells. Adherent cells can either grow as individual cells in 96...concentration exceeds ~100,000 cells/ml or for adherent cells, the cells have reached ~80% confluence....Continue to step 7.3 for suspension cells. For adherent cells, aspirate media. Add 20 μl of 0.05% trypsin-EDTA...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...plasmid expressing SpCas9 alone without sgRNA. Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase...Library CRISPR page for more information. Full references are below. Return to top Cas9 SAM transcriptional...The MS2-P65-HSF1 activation helper protein Full references are below. Pooled genome-wide human SAM library...Swiech et al., Nature Biotechnology 2014). Full references are below. Available plasmids are described below...The vector can be digested using BsaI ( This is different from BbsI, which is used in the SpCas9 plasmid...with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin mouse can be purchased...This plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase and sgRNAs targeting...

  4. Bacterial Expression Systems

    Type
    Collection
    ...metabolite levels. Different plasmids and, often times, different bacterial strains have different levels of precision...produce a therapeutic compound, there are many different ways to alter bacterial genomes. From recombineering... pRSETa mEos2 20341 mEos2 (fluorescent protein) Loren Looger Photoactivable Fluorescent Protein Expression...protein expression. Vectors come with and without different combinations of His and Strep tags as well as ...control of gene expression so be sure to try a few different systems with your gene/protein of interest. Plasmid...to 3OC6HSL. EsaR derivatives found herein have different sensitivities to 3OC6HSL. The EsaR-responsive ...site compatible with standard cloning using many different restriction enzymes. pPro24-gfp contains GFP but...

  5. Optogenetics AAV Preps

    Type
    Collection
    ...pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE CamKII ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Deisseroth...pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Deisseroth...pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE EF1a ChRmine (high-photocurrent, red-shifted) mScarlet Cre dependent 1, 5 Deisseroth... 6m-p2A-ChRmine-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) GCaMP6m Constitutive 8 Deisseroth... pAAV-nEF-ChRmine-mScarlet nEF ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Deisseroth...pAAV-nEF-Con/Fon-ChRmine-oScarlet nEF ChRmine (high-photocurrent, red-shifted) oScarlet Cre and Flp dependent...-Coff/Fon-ChRmine-oScarlet nEF ChRmine (high-photocurrent, red-shifted) oScarlet Flp dependent 8 Deisseroth...

  6. Mammalian RNAi Tools

    Type
    Collection
    ...Overview of shRNA-mediated RNA interference. Created with BioRender.com. shRNA Plasmids Browse our selection...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...Tools for RNAi Browse Plasmids Resources RNA interference (RNAi) is an RNA-mediated gene silencing mechanism...pSico with pSicoligomaker (Andrea Ventura lab) References Carthew, R. W., & Sontheimer, E. J. (2009). Origins...J. (2009). siRNA vs. shRNA: Similarities and differences . Advanced Drug Delivery Reviews, 61 (9), 746... Tuning silence: conditional systems for RNA interference . Nature Methods, 3 (9), 682–688. PubMed (Link...

  7. Genetic Code Expansion

    Type
    Collection
    ... directed evolution to charge the tRNA with a different amino acid. They are typically expressed from ...coli p-acetyl-l-phenylalanine (pAcF) Bacterial TAG Farren Isaacs 73545 pEvol-pAcFRS.1.t1 pAcFRS.1.t1 E. coli...coli p-acetyl-l-phenylalanine (pAcF) Bacterial TAG Farren Isaacs 73546 pEvol-pAzFRS.2.t1 pAzFRS.2.t1 E. coli...coli p-azido-l-phenylalanine (pAzF) Bacterial TAG Farren Isaacs 73547 pEvol-pAzFRS.1.t1 pAzFRS.1.t1 E. coli...coli p-azido-l-phenylalanine (pAzF) Bacterial TAG Farren Isaacs 82417 pUltra-sY sY-specific aaRS M. jannaschii...EAziRS) p-Azido-phenylalanine (Azi) Mammalian TAG Irene Coin 105830 pNEU-hMbPylRS-4xU6M15 PylRS M. barkeri...-like click amino acids, tRNA M15 Mammalian TAG Irene Coin 113644 pRF0G-Tyr tyrosyl-tRNA synthetase M....

  8. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...amino acids on either side occurring within the parent protein. Using the recoded strain of E. coli ( ...performing phosphoproteomic experiments in need of reference spectra or phosphopeptide standards for quantitative...FACS). The mode #2 phosphosite library with four different phosphobinding domains (14-3-3β, 14-3-3σ, the ...phosphorylation positional isomers are allocated to different subpools, allowing precise knowledge of phosphorylated...Library 111708 Mode #2 Library (NEDD4-2 WW2 domain) References & Protocols Iterative Synthetically Phosphorylated...Hohn MJ, Umehara T, Guo LT, Osborne EM, Benner J, Noren CJ, Rinehart J, Söll D. Science . 2011 Aug 26;333...

  9. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Beta-catenin GFP Adherens Junctions Beta-catenin EGFP Alpha Yap 67937 mouse E-cadherin GFP (423) Adherens Junctions...interest with a fluorescent protein that has a different emission wavelength. For example, if the marker...stress. There are four types of cell junctions: adherens junctions, gap junctions, tight junctions, and...EGFP Alpha Yap 71366 Murine E-cadherin mCherry Adherens Junctions E-cadherin mCherry Alpha Yap 54219 mEmerald-Paxillin...Michael Davidson 55001 mCherry-Beta-Catenin-20 Adherens Junctions Beta-Catenin mCherry Michael Davidson... 24920 pEGFP-LC3 (human) Autophagosome LC3 EGFP Toren Finkel 14435 mRFP-Clc Clathrin-coated vesicles Clathrin...

  10. Plan Your Experiment

    Type
    Collection
    ...Desired Genetic Manipulation Different genetic manipulations require different CRISPR components. Selecting...experimental goal, you are ready to start navigating the different reagents that are available for your particular...targeting promoter elements of target gene Many different activators exist, including the multi-plasmid ...two mutated alleles, each mutated allele may be different owing to the error-prone nature of NHEJ. In HDR... is PCR amplified, PCR products are denatured-renatured, treated with a nuclease that cleaves DNA heteroduplexes...
Showing: 11 - 20 of 97 results