Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 51 results for: Cas9

Showing: 21 - 40 of 51 results

  1. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Tools CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes... CRISPR dCas9-FokI CRISPR: dCas9-FokI...with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...gRNAs, binding ~15-25 bp apart, are required for dCas9-FokI to dimerize at a given region of the genome...

  2. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... to immunoprecipitate FLAG-tagged Cas9. In the CAPTURE system, Cas9 is tagged with a biotin acceptor site... Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of genomic DNA...Publication Visualize A catalytically inactive Cas9 (dCas9) fused to a fluorescent protein (FP) can help...Promoter Selectable Marker Publication Prime Edit Cas9 H840A nickase fused to a reverse transcriptase (...PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors... CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a ...

  3. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...in retinal development the lab utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate...taken advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several...fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids... plasmids dCas9-KRAB-MeCP2 fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion...175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572 express dCas9-VPR for activation...Sources Original pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2 ( Addgene #110821 ) VPR ( Addgene #63798...

  4. AAV Molecular Tools

    Type
    Collection
    ...Engineering Tools These AAV encode Cas9 nuclease used in the CRISPR-Cas9 system for targeted genome editing...AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA CMV-driven, constitutive Expression of human codon-optimized Cas9 from Staphylococcus...Staphylococcus aureus (SaCas9). 6 Zhang Labeling Tools These AAV encode tools that can be used for cell...

  5. CRISPR Plasmids - Plants

    Type
    Collection
    ... Plasmid Promoter Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI 46968 pICSL01009::AtU6p aU6...Promoter Selectable Marker PI Publication Prime Edit Cas9 H840A nickase fused to a reverse transcriptase (...BsaI yes, cut S. pyogenes Hygro Chen 78932 pHDE-35S-Cas9-mCherry-UBQ U6-26 PmeI yes, cut S. pyogenes Hygro...PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...Marker PI Publication Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a ...Marker PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can...

  6. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...species or PAM binding variant of Cas9. For instance, wild-type SpCas9 must be used with targets that are...increases off-target effect. Which species or variant of Cas9 ( S. pyogenes , S. aureus etc.) was this gRNA sequence...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  7. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...genomic deletions. Compared to deletions generated by Cas9, large deletions created using Cas3 have fewer off-target...underutilized compared to their Class 2 counterparts like Cas9. This is attributed in part to the difficulties ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  8. Validated gRNA Sequences

    Type
    Collection
    ...single species or PAM binding variant of Cas9 (e.g. Wild-type SpCas9 must be used with targets that are upstream...activity. Know your target. Which species or variant of Cas9 ( S. pyogenes, S. aureus etc.) was this gRNA sequence...and Resources page have been used to indicate the Cas9 application the gRNA was designed to accomplish....Target Species Target Sequence Plasmid ID Application Cas9 Species PubMed ID Depositor OCT4 H. sapiens CTCCCATGCATTCAAACTG...10 C. elegans CGCTACCATAGGCACCACG 71516 cut VRER Cas9 mutant 26680661 Fire dpy-10 C.elegans GCTACCATAGGCACCACGAG...-10 C. elegans TCCGCTACCATAGGCACCA 71479 cut VQR Cas9 variant 26680661 Fire dpy‐10 C. elegans GCTACCATAGGCACCACGAG...Christiaen EGFP A. victoria multiple, see article 60071 dCas9-FokI S. pyogenes 24770325 Joung EGFP A. victoria...

  9. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ... for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved by fusing a biotin...inactive dCas9 is used to purify genomic DNA bound by the gRNA. An epitope tag(s) can be fused to dCas9 or ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system. The ...purification, design your gRNA sequence to direct dCas9 to a specific locus, avoiding known transcription...

  10. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require that each DNA target have...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  11. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ... inactive Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have turned dCas9 into a customizable...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  12. CRISPR Plasmids - Parasites

    Type
    Collection
    ...research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and forward...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for some...

  13. Neurodegeneration Research Collection

    Type
    Collection
    ...Target neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent rodent...species. Boender et al. Sci Adv. 2023 Jun 2. Use Cas9 in astrocytes. Endo et al Science. 2022 Nov 4. Base...

  14. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink Lab Materials...

  15. Distribution to Industry

    Type
    Collection
    ...Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome editing of human TP53...

  16. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... Separate expression pRGEB31 - Deliver sgRNA and Cas9 into plants by Agrobacterium-mediated transformation...Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated... FP Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions...

  17. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...require the use of lentivirus to deliver the gRNA/Cas9 to target cells. Therefore, you must be equipped...86550 Inhibition Human Weissman 3rd 10 Varies CRISPR/Cas9-assisted Removal of Mitochondrial DNA (CARM) Library...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors... Sabatini and Lander 3rd 50 6,661 Garnett Lab MinLibCas9 Library 164896 Knockout Human Garnett 3rd 2 37,722...

  19. Rett Syndrome

    Type
    Collection
    ...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of additional...

  20. Bacterial Expression Systems

    Type
    Collection
    ...synthesize a target metabolite, genetic tools like CRISPR-Cas9 are not only valuable but often essential. Find ...Plasmid Promoter Inducer Expression Species PI 44249 pdCas9-bacteria pTetO Anhydrotetracycline (aTc) Escherichia...
Showing: 21 - 40 of 51 results