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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 28 results for: ENDO 2

Showing: 21 - 28 of 28 results

  1. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...sequence YFP Thorben Dammeyer 54520 mCherry-Peroxisomes-2 Peroxisome Peroximal Targeting Signal 1 (PTS1) mCherry...Centrin2 Centrioles (dependent on cell cycle) Centrin-2 EGFP Erich Nigg 41151 pEGFP Cep170 C-term Centrioles...14437 mRFP-Rab5 Early endosomes RAB5A mRFP Ari Helenius 61801 GFP-Rab4B Early endosomes Rab4B AcGFP Gia Voeltz...49201 mCh-Rab5 Early endosomes Rab5 mCherry Gia Voeltz 49147 BFP-Rab5 Early endosomes Rab5 BFP Gia Voeltz...GFP-Rab5B Early endosomes Rab5B AcGFP Gia Voeltz 79802 pTag-RFP-C-h-Rab5a-c-Myc Early endosomes Rab5a TagRFP...pTag-BFP-C-h-Rab5a-c-Myc Early endosomes Rab5a TagBFP James Johnson 13050 DsRed-Rab5 WT Early endosomes RAB5A DsRed2 Richard...GFP-EEA1 wt Early endosomes EEA1 GFP Silvia Corvera 42635 TagRFP-T-EEA1 Early endosomes EEA1 TagRFP-T Silvia...

  2. CRISPR References and Information

    Type
    Collection
    ...Link opens in a new window) An encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme...library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro... Mendenhall and Myers Mammalian: FLAG tagging endogenous proteins pFETCh_Donor ; additional HDR & gRNA...

  3. Tetracycline Inducible Expression

    Type
    Collection
    ...hSyn promoter rtTA3 Wei Xu 26803 pEnt L1L3 EF1a-tTA-2 Gateway entry vector to express tTA from EF1α promoter...expression and improved dynamic range . Hum Gene Ther, 22 (2), 166–176. https://doi.org/10.1089/hum.2010.099 (Link.... To avoid secondary effects from repurposing endogenous systems that respond to natural stimuli like ...al., 2011), engineered to eliminate binding of endogenous mammalian transcription factors to further minimize...their sequence and eliminating interactions with endogenous factors. Experimental Considerations Expression...

  4. Antibody Production

    Type
    Collection
    ...for shipping stability by incubating at 37 °C for 2 weeks, freezing at -80 °C, thawing on ice, and then... been retained. If the target antigen is not endogenously-expressed (e.g., using an anti-GFP antibody)...

  5. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...transient mammalian expression 101126 pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient...pSNAP-CaaX Control Plasmid SNAP-tag Control for endomembrane (CaaX box) localization 101129 pSNAPf-Cox8A ...

  6. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme...BclI-SwaI none S. pyogenes URA3 Wyrick pCRISPomyces-2 61737 Bacteria BbsI yes, cut S. pyogenes Apramycin... S. pyogenes Zhang gRNA Cloning Vector Bbs I ver. 2 85586 Mammalian BbsI None S. pyogenes H Fujii pX601...CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system Plant Utilizes the tRNA... promoters. Bullock pCFD5 Drosophila Utilizes endogenous tRNA processing machinery to excise multiple ...

  7. Luciferase Plasmid Collection

    Type
    Collection
    ...fusing NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. Nano-lanterns , Enhanced Nano-lanterns...transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase...protein interactions , protein dimerization , endosomal escape of cell-penetrating peptides , and more...

  8. Plan Your Experiment

    Type
    Collection
    ...either (1) a lack of gRNA and/or Cas9 expression or (2) a lack of efficient target cleavage in cells expressing... Activate (CRISPRa) Increase expression of an endogenous gene(s) without permanently modifying the genome...
Showing: 21 - 28 of 28 results