We narrowed to 80 results for: expression vector

...targeting vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general... one vector from the Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which expresses an untagged...gene-specific CRISPR and donor vectors as well as pX330S-2-PITCh, expressing a generic gRNA targeting the...Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific...are available. An improved FLP recombinase expression vector allows for efficient marker cassette FLP-out...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors By Species Mammalian Bacteria Drosophila Plant ...has been adapted to allow tagging of proteins expressed from their natural chromosomal context. These ...

...Plasmids Viral Vectors pLEG/pREG Modular Viral Vector System - Dankort Gene Vector Core Viral Vectors - Oka...research, visualization, protein expression, synthetic biology, and viral vector plasmids. Plasmid...to drive selective expression in the brain - Simpson Reagents for improved expression of recombinant phosphoproteins...Collection Botman-Teusink Yeast FP Collection Protein Expression Cell Migration Consortium Malate Dehydrogenase...Open Enzyme Collection Screening Multiplexed Overexpression of Regulatory Factors (MORF) Collection Synthetic...

... Viral Vector Packaging Service AAV Molecular Tools Viral Vector Packaging Service: Molecular...with tetracycline (tet)-inducible expression systems . ID Name Expression System Activity Serotype PI 99118...transsynaptic tracer protein to express in presynaptic glutamatergic neurons that express Cre, Flp payload. Must...Cre-dependent expression of ER-localized TurboID (with HA and V5 tag). 8 Jonathan Long Overexpression Tools These...These AAV encode various genes for overexpression. ID Name Expression System Activity Serotype PI 58376...99118 pAAV-CAG-tTA CAG-driven, constitutive Expression of the tet-off transactivator (tTA). 2 Viviana Gradinaru...pAAV-ihSyn1-tTA Inducible Synapsin promoter (ihSyn) Expression of the tet-off transactivator (tTA) with a positive...

.... pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS),...fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed. EXPRESs...in zebrafish. Zebrafish expression atlas - A zebrafish-specific gene expression database that provides ...fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large collections or kits are described...CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples...found below: UFlip CRISPR targeted integration vectors for cell-type specific conditional gene inactivation...inactivation - Jeffrey Essner Lab GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner...

...more details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled...4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau Viral Service...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently ... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus...52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...Zhang Cas9 and Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused ...61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A GFP Zhang 61425 lenti...

... Viral Vector Packaging Service AAV Retrograde AAV Viral Vector Packaging Service: Retrograde...Color-flipping switch. Expresses NLS-mCherry in the absence of Cre, and expresses NLS-EGFP in Cre-positive... Cre expression Recombinases James M. Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn Cre expression Recombinases... Cre expression Recombinases James M. Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre expression with ...simultaneous dTomato expression Recombinases Rylan Larsen 107788 AAV.rTH.PI.Cre.SV40 rTH Cre expression Recombinases...physically-separate mCherry expression Recombinases Karl Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases...EF1a Flpo expression Recombinases Karl Deisseroth 55638 pAAV-EF1a-vCre EF1a VCre expression Recombinases...

...Barbier Lab Cloning and Expression Bacteria and yeast expression vectors : FX cloning System - Geertsma and... E. coli expression - Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier...- Rossignol Lab Yeast Gateway kit : 285 vectors for expression, markers, fusion proteins, etc - Lindquist...Sets of plasmids for fluorescence, cloning and expression, and synthetic biology. CRISPR Resources : Find...plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied as one item. ...Santalucia Labs pCri System : Heterologous protein expression in E. coli, B. subtilis and P. pastoris - Gomis-Rüth...constructs - Lahaye Lab Vaccinia virus ORF library : Expression library that contains each of the predicted open...

...General Tools Fire Lab C. elegans Vector Kit - Andrew Fire Lab. A set of vectors for C. elegans research, including...and spatial control of gene expression in C. elegans by combining expression of the FLP recombinase from...can knock down genes by feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily...developed and characterized which allow researchers to express desired constructs in precise cell types and/or...empty backbones, fluorescent proteins, and Cre expression plasmids. Large collections or kits are described...ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids...plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied as one item. ...

....org Adenoviral Vector Guide All Viral Plasmids Viral Vectors 101 eBook Viral Vectors 101 YouTube Videos... can be used for adenoviral vector production. Read our adenoviral vector guide for more information about...Adenoviral Vector Guide Shuttle Plasmids Browse commonly used shuttle plasmids for adenoviral vector production...adenoviral vector production. ID Plasmid / Kit Description PI 16400 pAdEasy-1 Adenoviral vector for use ... plasmids, and bacterial strains for adenoviral vector production in Addgene's repository. Viral...Adenoviral Plasmids Adenoviral Strains Adenoviral vectors are widely used for gene delivery due to their ...high efficiency in driving strong transient gene expression in both dividing and non-dividing cells, and ...

... 101137 pSNAP-tag (T7) Vector SNAP-tag Empty backbone for bacterial expression under T7 control 101135...pSNAP-tag (m) Vector SNAP-tag Empty backbone for stable and transient mammalian expression 101133 pSNAP-CaaX...pCLIP-tag (m) Vector CLIP-tag Empty backbone for stable and transient mammalian expression 101134 pCLIPf-H2B...101127 pMCP-tag (m) Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag ...pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient mammalian expression 101132 pMCP-GPI...Synthases (AcpS and Sfp, respectively) may be expressed and purified as described previously in Stachelhaus...

... Viral Vector Packaging Service AAV Monosynaptic Neuronal Tracing Viral Vector Packaging Service... Addgene does not distribute rabies virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description...direct virus infection specifically into cells that express the TVA viral receptor. Thus, by pseudotyping the...virus with EnvA, only neuronal cells engineered to express TVA will be infected. Schematic depicting steps...to the starter cell. (2) The starter cell now expresses TVA and G-protein. EnvA-pseudotyped RAVB is delivered... via G-protein receptors, which are naturally expressed at presynaptic nerve terminals. (4) Neurons projecting...G-protein coated RABV. These projection neurons do not express G-protein, and thus do not give rise to G-protein...

... Viral Vector Packaging Service AAV Caltech Systemic Capsids Viral Vector Packaging Service... a new window) . Viral Vectors 101: Systemic Capsids PHP.eB These viral vector preparations were produced...pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn Cre-EGFP expression Cre Wilson 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP Cre-expression Cre Wilson 107738...CN4825) minBG iCre(R297T) expression iCre AIBS , Ting PHP.S These viral vector preparations were produced... CAP-B10 CAP-B22 AAV9-X1.1 As part of our Viral Vector Packaging Service , Addgene is distributing ready-to-use...of our standard viral production , these viral vectors undergo quality control, including AAV titering...testing, and full sequencing of the final viral vector preparation. Systemic Delivery AAV Capsid Choice...

...cleavage, Bac-to-Bac Expression System, AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins...TEV cleavage, AmpR 26095 pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine...SGC Toronto SGC Oxford SGC Stockholm Bacterial Expression Plasmids (empty backbones/controls) ID Plasmid...Thrombin cleavage, Bac-to-Bac Baculovirus Expresion vector 26117 pNIC-CH EF199843 Hexahistidine tag, AmpR ...hexahistidine tag and TEV cleavage, Bac-to-Bac Expression System, AmpR Related Resources SGC: www.thesgc.org...

...luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing tags such...luciferase reporter vectors for sensitive gene expression analysis and protein fusion vectors for detecting ... from an enzymatic reaction to measure subtle expression changes with high sensitivity. These assays are...enables highly sensitive detection at endogenous expression levels. NanoLuc fusions are useful for both intracellular...want minimal disruption of endogenous protein expression and interactions, such as exploring viral biology...HaloTag technology is optimized for various protein expression systems including E. coli , mammalian cells, ...interest is fused to either LgBiT or SmBiT and expressed in live cells. When the two proteins interact,...

...-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons. For...version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons...sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells The Allen...Fan 221463-AAV1 pAAV_BiPVe4_eGFP AAV construct expressing eGFP driven by chandelier cell-targeting enhancer...Wilson 221463-PHPeB pAAV_BiPVe4_eGFP AAV construct expressing eGFP driven by chandelier cell-targeting enhancer...-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5140) Expression of CoChR-EGFP in striatal cholinergic neurons..._3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) Expression of iCre(R297T) in striatal medium spiny neurons...

...These proteins can be expressed in specific neurons, often utilizing viral expression systems, to activate...Insert Vector Type Promoter PI Return to top Additional Resources Optogenetics Guide Viral Vector Packaging...and kinetic properties as well as improve the expression and membrane targeting in host cells. Other microbial...fluorescent protein to the opsin in order to track its expression and localization. Many other types of proteins...optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for Viral Packaging PI Return...

...Assembly method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease...Find reagents for engineering and expressing zinc finger proteins deposited by Zinc Finger Consortium... Addgene various reagents for engineering and expressing zinc finger proteins for distribution to academic...

...enzymes into an expression vector of their choice and then transform into E. coli for expression and purification...their own enzymes by cloning them into an expression vector of their choice and transforming into E. coli... and protocols for expression in the Open Bioeconomy Lab's crowd-sourced expression guides (Link opens...Return to top Additional Resources Bacterial Expression Vectors Synthetic Biology Plasmids MoClo Guide Drew...and codon-optimized the Open Enzymes parts for expression in Escherichia coli . Researchers can use the...purification ( pET28a-LIC or other pET vectors are recommended). The selection marker for all plasmids in ... are also compatible with the E. coli Protein Expression Toolkit (Link opens in a new window) — all Open...

... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Transducibility Some viral vectors are tested in vitro and in vivo for gene expression and/or function. These...Empty vector particles can be identified after negative staining and appear darker than full vector particles...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles... Viral Vector Packaging Service Viral Production Viral Production...to determine which facility produced your viral vector lot. At Addgene, AAV particles are titered by droplet...

... Viral Vector Packaging Service AAV Serotype Testing AAV Viral Vector Packaging Service: ...available from Addgene's viral service. Control EGFP vectors in various serotypes for serotype testing: AAV1...addition to the standard 100 µL sample size, the vectors listed below are also available as 20 µL. The ...does not have the suffix (for example, AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825... (deposited by Edward Boyden ) and direct GFP expression from the CAG promoter. For information about ...50465 (deposited by Bryan Roth ) and direct EGFP expression from the human synpasin promoter. For information... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...