...plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table and search "lenti....1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid; includes a stuffer for easy cloning Root 14748...Kasparov 1732 pCPRDEnv 2nd Packaging part of the FELIX vector system, expresses Gag-Pol and Rev Nolan 35617 pCAG-Eco...shRNA. See article for detailed information about cloning & additional plasmids. Aebischer and Trono 11795...cassette (promoter and gene of interest) can be cloned into the plasmid. Nolan 12257 pWPXL 2nd EF-1alpha... the gene of interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in...

...General Tools Fire Lab C. elegans Vector Kit - Andrew Fire Lab. A set of vectors for C. elegans research, including...sets of plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied ...resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging...plasmids that streamlines the production of targeting vectors for tag insertion using CRISPR/Cas9 and the selection...editing in C. elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified...including lacZ and GFP fusion vectors. C. elegans optimized fluorophores - Andrew Fire Lab. Available ...

...come in 1-vector systems, in which Cas9 is included in the gRNA-containing plasmid, or 2-vector systems,... of unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high ...sequence is cloned into a gRNA expression plasmid but does NOT include the PAM sequence or the gRNA scaffold... guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic...commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the gRNA that is responsible for...amplify a specific sequence of DNA pegRNA P rime E dit gRNA; a specialized gRNA containing an additional primer...Cas enzyme and a gRNA specific to the gene of interest. The genomic target of the gRNA can be any ~20 nucleotide...

... view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology...libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A modular set of plasmids ...Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find a scientific community that has...

.... pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS),... selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR...found below: UFlip CRISPR targeted integration vectors for cell-type specific conditional gene inactivation...inactivation - Jeffrey Essner Lab GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner...mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish...with fluorescent proteins to visualize and track clones of cells over time. Examples of useful tools for...with four promoters, six fluorophores, and empty vectors designed to streamline the production of transgenic...

...CRISPR/Cas9 Clones for Deletions and Clone Selection For suspension cells, transfer all clones to a single...deletion clones with a proofreading polymerase and clone the amplicons ( e.g., with a PCR cloning kit) into... the guide’s reverse complement for cloning into the pX330 vector using BbsI restriction enzyme ( Table...Gate assembly cloning strategy 3 . Prepare a 50 μl reaction mix: 100 ng circular pX330 vector, 1.0 μl annealed...one plate to allow for clones to grow and use the other plate to screen each clone for deletions. Add an...Validation of Biallelic Deletion Clones In order to characterize obtained clones and validate a successful knockout... knockout, two sgRNA located within exons will enrich even monoallelic deletion clones for loss of function...

... of interest are cloned into a Firefly luciferase reporter vector via Gateway cloning to test for the ...regions Joshua Mendell 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...Renilla and Firefly luciferase. Plasmids are Gateway cloning compatible. Learn more about DULIP in our 27 Hot...collection of empty backbone plasmids into which you can clone your regulatory element or gene of interest into... of 5' promoter/enhancer regions using Gateway cloning. Lentiviral. Jorge Ferrer 87067 pcDNA3.1-ccdB-Nanoluc...Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 87075 pLenti6.2-ccdB-Nanoluc NanoLuc...Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival. Mikko Taipale 87078 pLenti6.2-Nanoluc-ccdB...

...expression when cloning in E. coli . This alteration enables the generation of a single vector containing ...Cre-ERT2 - Tamoxifen inducible; Gateway entry vector none Entry Vector Zon 27493 pBT140 Cre CMV Mammalian Luo...Cre-ERT2;Targeting vector for Fos locus Mammalian Luo 46389 pArcCreER Cre-ERT2;Targeting vector for Arc locus...Burgess 61570 Pvalb-2A-Cre targeting vector Cre; Targeting vector for Pvalb locus Mammalian Zeng 61573...61573 Rasgrf2-2A-dCre targeting vector DHFR-destabilized Cre; targeting vector for Rasgrf2 Mammalian Zeng ...Zeng 61574 Slc17a7-IRES2-Cre targeting vector Cre; Targeting vector for Slc17a7 locus Mammalian Zeng 62219...Nxph4-2A-CreERT2 Targeting Vector Cre-ERT2 - Tamoxifen inducible; Targeting vector Mouse Nxph4 Mammalian Zeng...