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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25 results for: grna cloning vector

Showing: 21 - 25 of 25 results

  1. Plasmid Collections

    Type
    Collection
    ... view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology...libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A modular set of plasmids ...Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find a scientific community that has...

  2. Zebrafish Plasmid Collection

    Type
    Collection
    .... pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS),... selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR...found below: UFlip CRISPR targeted integration vectors for cell-type specific conditional gene inactivation...inactivation - Jeffrey Essner Lab GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner...mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish...with fluorescent proteins to visualize and track clones of cells over time. Examples of useful tools for...with four promoters, six fluorophores, and empty vectors designed to streamline the production of transgenic...

  3. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...CRISPR/Cas9 Clones for Deletions and Clone Selection For suspension cells, transfer all clones to a single...deletion clones with a proofreading polymerase and clone the amplicons ( e.g., with a PCR cloning kit) into... the guide’s reverse complement for cloning into the pX330 vector using BbsI restriction enzyme ( Table...Gate assembly cloning strategy 3 . Prepare a 50 μl reaction mix: 100 ng circular pX330 vector, 1.0 μl annealed...one plate to allow for clones to grow and use the other plate to screen each clone for deletions. Add an...Validation of Biallelic Deletion Clones In order to characterize obtained clones and validate a successful knockout... knockout, two sgRNA located within exons will enrich even monoallelic deletion clones for loss of function...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...popular empty backbone plasmids into which you can clone your regulatory element or gene of interest into... of 5' promoter/enhancer regions using Gateway cloning. Jorge Ferrer 16539 pBV-Luc Firefly Insertion of...Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest upstream...Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 87075 pLenti6.2-ccdB-Nanoluc NanoLuc...Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival. Mikko Taipale 87078 pLenti6.2-Nanoluc-ccdB...Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X Firefly...

  5. Tetracycline Inducible Expression

    Type
    Collection
    ...plasmid. A single vector approach may not have as much flexibility and control as a dual vector design, but ...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or search...tet-inducible vectors. Tetracycline-inducible Backbones Find backbones or constructs to use for cloning to control....1 Lentiviral Tet-On vector for inducible expression; insert with Gateway cloning and puromycin selection...Lentiviral Tet-Off vector for repressible expression with blasticidin selection. *Note: this vector contains an...respectively. You should also choose between: Dual vector system: express the transactivator and the tet-...-controlled gene of interest from two separate vectors. Stable cell lines or transgenic animals can be...
Showing: 21 - 25 of 25 results