...be lysed or the substrate must be delivered intracellularly in order to measure luminescence. Gaussia luciferase...luciferase are less-than-ideal protein tags due to their large size. NanoLuc® Luciferase is small, bright, and ...protein is available – these attributes have made it popular with scientists since its release. Luciferase-containing...Resonance Energy Transfer (BRET) CalFlux VNT : An intracellular calcium sensor created by fusing NanoLuc® and...allow for BRET-based in vivo imaging of multiple cellular processes, biosensors, or optogenetic tools . ... (Rluc8) through an Ala-Glu-Leu linker to the circularly permuted Venus fluorophore. Learn more about ...Gateway cloning for expression in plants Andrew Millar 159707 pG20_LUC2_Hyg Firefly Creation of C-terminal...

...genetically engineered cellular receptors that affect signalling pathways within cells. Subcellular Localization...Monitor small biomolecules or other physiological intracellular processes with genetically encoded fluorescent...Guide Use light to detect, measure, and control molecular signals, cells, or groups of cells with either...Fluorescent Proteins from Kurt Thorn and UCSF Molecular Expressions Galleries from Michael Davidson and...

...the sequence between the sites is deleted as a circular piece of DNA (and is not maintained). C) If the...13763 pTriEx-HTNC Cre fused to HIV-TAT - promotes cellular uptake of recombinant Cre Bacterial Rajewsky 13775...CMV-CFP;UBC-Cre25nt Cre with a 25 nucleotide extracellular vesicle targeting sequence and CFP UBC Mammalian...pAAV-hSyn-Cre-P2A-dTomato Cre and dTomato hSyn AAV Larsen 107787 AAV.TBG.PI.Cre.rBG Cre TBG AAV Wilson 107788...activates shRNA expression, removal of EGFP; See also similar plasmids pSico PGK GFP and pSico PGK puro Lentiviral...off shRNA expression, removal of EGFP; See also similar plasmids pSicoR PGK puro , pSicoR-Ef1a-mCh-Puro... to eGFP, removes dsRed. See article for other similar plasmids. Retroviral Clevers 15912 CTV Cre activates...

...LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression of Large T antigen for creation of immortalized...about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...Frequently asked questions (FAQ) about retrovirus Popular γ-Retrovirus Envelope and Packaging Plasmids Common...Envelope VSV-G-expressing envelope plasmid Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral...

...characteristics and evolutionary similarity. These are grouped into two large classes based on the structure...: An Adaptive Immune System CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) sequences...made in just five years, with the skyrocketing popularity of CRISPR echoing that of RNAi and iPS cells....Cell . 159(3):647-6. PMID: 25307932 Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-... WA, Weissman JS, Qi LS. 2013. CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes...

...immunogenic properties of the final product, particularly in large animal studies. Endotoxin contamination ...silver staining or SYPRO Ruby staining and the molecular weight and relative intensity of the viral capsid...lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a...virus protocols . Browse our plasmid collection of popular lentiviral vectors ....

...the world's largest biorepositories (Link opens in a new window) ATCC - the world's largest cell line repository...further downstream in the NID and C-terminus. A large number of mutations are known to cause Rett syndrome...accelerated our understanding of Rett syndrome at the molecular level. They are also an important tool for developing... PMID: 25801175 Tillotson and Bird. 2019. The Molecular Basis of MeCP2 Function in the Brain. J Mol Biol...

...serotype has been reported to occur by using the cellular receptor LY6A ( Huang et al., 2019 (Link opens...pAAV-hSyn-Cre-P2A-dTomato Syn Cre-expression Cre Larsen 140135 pAAV-EF1a-iCreV EF1a light-inducible recombinase... Cell type-biased: Neurons - AAV.CAP-B10 Brain vascular cells - AAV.CAP-Mac For rats: Broad tropism - ... Cell type-biased: Neurons - AAV.CAP-Mac Brain vascular cells - AAV9-X1.1, AAV1-X1 For mice: Broad tropism...AAV-PHP.eB with GRE or transgenic animal Brain vascular cells - AAV9-X1.1, AAV1-X1 Neurons - AAV.CAP-B10...

...that can cause large genomic deletions. Compared to deletions generated by Cas9, large deletions created...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR... While abundant in nature, Class 1 systems are largely underutilized compared to their Class 2 counterparts...

...cleavage of the target. RNAi using siRNA follows a similar mechanism. Since it is pre-processed, it can bypass.... M., Abadi, S. J., & Gharagouzlo, E. (2017). Molecular mechanisms and biological functions of siRNA ....and assessment of gene knockdown . Methods in Molecular Biology, 629 , 141–158. PubMed (Link opens in ...., & Nemunaitis, J. (2009). siRNA vs. shRNA: Similarities and differences . Advanced Drug Delivery Reviews...

...Browse CRISPR pooled libraries for large-scale functional screens. Available for knockout, activation...representation. CRISPR screening experiments require large quantities of cells and therefore, may not be suitable...important to know which library best suits your particular experiment. Some important variables include:...given CRISPR library are unique to the genome of particular organism, and the library is only compatible ...Cherry Brie Pooled Library 170511 Knockout Mouse Tolar 3rd ~4 78,637 Mouse Chromatin Regulator Library ...

...Advantages of adenoviruses in research include their large packaging capacity (>8 kb), high titers, and high... For recombining shuttle plasmids; for use with large inserts Vogelstein 179201 pAd5-B6 ∆E3-GFP Adenoviral...Venus Wang Adenoviral Kits Plasmids containing modular inserts that span the human adenovirus serotype...1000000176 AdenoBuilder toolkit Plasmids contain modular inserts for seamless assembly of multiple mutations...

...serotype also displays enhanced transduction of the trabecular meshwork following intracameral injection in ... Chamber Results in Efficient Transduction of Trabecular Meshwork in Mouse and Rat. PLoS One . Jun 8;10...AAV-mediated transduction and targeting of retinal bipolar cells with improved mGluR6 promoters in rodents...Intravitreal delivery of a novel AAV vector targets ON bipolar cells and restores visual function in a mouse model...

...nucleotide binding protein) family. GTPases serve as molecular switches that transmit signals within the cell...gene page are provided below. Color is used for clarity and does not indicate a specific relationship. ...Laboratory for Cancer Research Color is used for clarity and does not indicate a specific relationship. ...containing protein 1 SCRIB Scribbled planar cell polarity protein SHC SHC1 SHC2 SHC3 SHC4 SHC (Src homology...

... of the viral spike (S) protein to cellular receptors. The cellular receptor for SARS-CoV-2 and SARS-CoV...activity in neutralization assays and links to popular envelope and packaging plasmids. Ginkgo Bioworks...platforms to reliably detect nucleic acids at the atomolar level. For more information read Addgene's Blog...

...Mortrud M, Yao S, Siverts LA, Larsen R, Gore BB, Szelenyi ER, Trader C, Balaram P, van Velthoven CTJ, Chiang...using various delivery methods including intracerebroventricular (ICV) delivery in newborns, and retro-orbital...Juneau Z, Kalmbach B, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, Leibly M, Lenz GH, Liang E, Lusk...

...Cascade-Cas3 generates large genomic deletions. Insert NEW CRISPR transposases insert large fragments of DNA...CRISPR experiment. CRISPR 101 eBook : Based on our popular CRISPR 101 blog series, we've organized a comprehensive...

...histidine-tagged constructs and routine protein and molecular biology techniques. Using a single protein with...species (e.g., human, watermelon, Streptomyces ) Subcellular compartment (chloroplastic, cytoplasmic, glyoxysomal...References for teachers Using Bioinformatics and Molecular Visualization to Develop Student Hypotheses in...

...GreenGate Cloning System (Plant) MoClo Modular Cloning Modular PUF Assembly Kit Optogenetics Pertz Lab...Sensing and Signaling Browse plasmids related to intercellular signaling and environmental sensing. Strains...

...Antibodies are large proteins made by the immune system that bind to an antigen, a particular part of a foreign...fragments that bind to the GCN4 peptide, from the popular SunTag system, fused to sfGFP for imaging. Learn...