Brzezinski Lab CRISPR Collection
Type
Collection
...guide construct. Plasmids 175573 and 175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572...guides can be expressed from the same plasmid by utilizing one of the dual guide shuttle plasmids to insert...reporter) pX458 plasmid family. They have made several modifications to the the pX458 plasmid to expand its...addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid for shuttling...shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2 fusion for gene and non-...activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids 159654 and 159655...159655 are single guide expressing double stranded break constructs. Plasmids 171098 and 175570 allow conversion...