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We narrowed to 36 results for: plasmids 101

Showing: 21 - 36 of 36 results

  1. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Broad GPP genome-wide Brunello 73179 (1 plasmid) 73178 (2 plasmid) Knockout Human Doench and Root 3rd 4 ...76,441 Broad GPP genome-wide Brie 73632 (1 plasmid) 73633 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ...GPP kinome Brunello 75314, 75315 (1 plasmid) 75312, 75313 (2 plasmid) Knockout Human Doench and Root 3rd... 3,052 Broad GPP kinome Brie 75317 (1 plasmid) 75316 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ...20,051 Human GeCKO v2 1000000048 (1 plasmid) 1000000049 (2 plasmid) Knockout Human Zhang 3rd 6 123,411...185,836 Mouse GeCKO v2 1000000052 (1 plasmid) 1000000053 (2 plasmid) Knockout Mouse Zhang 3rd 6 130,209... - 3 plasmid system 1000000075 (Puromycin) Activation Mouse Zhang 3rd 3 69,716 SAM v2 - 2 plasmid system...

  2. Plan Your Experiment

    Type
    Collection
    ...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile thanks...Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells...different activator systems exist, including the multi-plasmid SAM system Activate Design and Clone Your gRNA ..., anneal, and insert the targeting oligos into plasmids containing the gRNA scaffold using standard restriction-ligation...review the protocol associated with the specific plasmid in question (see CRISPR protocols from Addgene ...the expression time (transient or stable). For plasmid and viral-based approaches, the species and expression...

  3. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Neurodegeneration Neurodegeneration Plasmids Neurodegeneration Plasmid Collection Addgene has a large collection...Browse all Neurodegeneration Plasmids Do you have suggestions for other plasmids that should be added to this...Browse neurodegeneration plasmids for research related to Alzheimer's Disease, ALS, Frontotemporal Dementia...collection of neurodegeneration plasmids for research related to Alzheimer's Disease (AD), Amyotrophic...others. The table below can be sorted by disease, plasmid type, mutations, and/or tags such as fluorescent...particular gene of interest, please search our full plasmid collection using the search bar at the top of this...Tags GFP FP other Flag GST His TEV Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral...

  4. Biosensor AAV Preps

    Type
    Collection
    ...retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....Looger 101060 pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Syn CaMPARI2 his Constitutive 1 Schreiter 101061 pAAV_hsyn_NES-his-CaMPARI2...Syn CaMPARI2-F391W his Constitutive 1 Schreiter 101064 pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40 Syn ...

  5. mTOR Pathway

    Type
    Collection
    ...proliferation. mTOR Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's plasmids for the mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates...with a New Screening Kit Background mTOR Pathway Plasmids mTOR Gene List Resources The m echanistic or m... - Gene List Click on a name to find available plasmids for the gene. For components with multiple isoforms...Sabatini DM. Cell. 2012 Apr 13;149(2):274-93. doi: 10.1016/j.cell.2012.03.017. PubMed PMID: 22500797 . Rapamycin...Blenis J. Cell Metab. 2014 Mar 4;19(3):373-9. doi: 10.1016/j.cmet.2014.01.001. PubMed PMID: 24508508 . Targeting...

  6. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...SepOTSλ Plasmid 68291 SepOTSκ Plasmid 68290 SepOTSι Plasmid 68289 SepOTSθ Plasmid 68288 SepOTSη Plasmid 68287...S218TAG Plasmid 68300 MBP-MEK1 Plasmid 68299 GFP E17TAG/Q157TAG Plasmid 68298 GFP S2TAG/E17TAG Plasmid 68297...GFP Q157TAG Plasmid 68296 GFP S2TAG Plasmid 68295 GFP E17TAG Plasmid 68294 SepOTSν Plasmid 68292 SepOTSλ...68287 SepOTSζ Plasmid 68286 SepOTSϵ Plasmid 68285 SepOTSδ Plasmid 68284 SepOTSγ Plasmid 68283 SepOTSβ Plasmid....ΔA.Δserb.Amp Plasmid 68305 Beta lactamase S68TAG Plasmid 68302 MBP-MEK1 S222TAG Plasmid 68301 MBP-MEK1...pSerOTS-C1* (V70) (Plasmid #188537) a better choice in place of the popular SepOTSλ (Plasmid #68292) . An important...-C1* (V70) is a ROP minus plasmid and is not compatible with other plasmids containing high copy ColE1...

  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Addgene Blog: Biosensors Luciferase Plasmids Optogenetics Plasmids Viral Service: AAV Biosensors Fluorescent...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Find plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other assays... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors...our catalog . Use the article links to find the plasmids associated with the article. We also offer ready-to-use...ready-to-use AAV preparations of select plasmids and AAV Packaged on Request for many more. Metal Ions ...C-terminal domain of troponin C Griesbeck Lab Twitch Plasmids Oliver Griesbeck Calcium erGAP3 (GFP-Aequorin ...

  8. Zhang Lab CRISPR Page

    Type
    Collection
    ...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested...adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described...sgRNA backbone for cloning new targeted plasmids. The plasmid can be digested using SapI, which will reveal...2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice.... doi: 10.1016/j.cell.2014.09.014. Epub 2014 Sep 25. PubMed . Return to AAV Cas9 mouse plasmids...

  9. The Pleiades Promoter Project

    Type
    Collection
    ...using the links to view individual plasmid webpages. These plasmids are described in Portales-Casamar ...Plasmids from the Pleiades Promoter Project - A regulatory toolbox of MiniPromoters to drive selective... Depositor Collections Pleiades Promoter Plasmids Pleiades Promoter Project The Pleiades Promoter...human MiniPromoters. Browse the Pleiades Promoter Plasmids using the table below. Additional information ... pEMS1371 EGFP/NLS Ple100 GRP pEMS1372 EGFP/NLS Ple101 GRP pEMS1373 EGFP/NLS Ple102 GRP pEMS1374 EGFP/...Ple232 TNNT1 pEMS1704 intron-lacZ/NLS Ple233 TNNT1 pEMS1101 EGFP/cre/NLS Ple233 TNNT1 pEMS1437 EGFP/NLS Ple234...

  10. Genetic Code Expansion

    Type
    Collection
    ...Generally, you will need two plasmids, as depicted in the figure below: A plasmid expressing the tRNA and ...Research Center . Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA ...Many of the plasmids also contain one or more copies of the cognate tRNA gene. ID Plasmid Synthetase Origin...Abhishek Chatterjee Browse Target Plasmids The table below highlights plasmids that contain genes with modified...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian... Plasmid Collections Genetic Code Expansion Genetic Code Expansion You may also like... GCE4All...tRNA synthetases are introduced into the cell on plasmids and the new amino acid is introduced in the media...

  11. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...gRNA Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome ... these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited.... Whether the plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that...none, need plasmid 65775 N. meningitidis Joung VVT1 65779 Mammalian BsmBI none, need plasmid 65776 S. aureus...expression of Csy4 (eg Plasmid 53369 ). Joung pX333 Mammalian Single all-in-one plasmid for tandem expression...types of plasmids available for use in this Golden Gate and Gateway based assembly method: plasmids for assembly...

  12. Validated gRNA Sequences

    Type
    Collection
    ...CRISPR experiments. This datatable is compiled from plasmids in Addgene's database as well as from sequences..., the categories described on Addgene's CRISPR Plasmids and Resources page have been used to indicate ...Datatable Target Gene Target Species Target Sequence Plasmid ID Application Cas9 Species PubMed ID Depositor...ACCATCTAATTCAACAAGAATT 73221 interfere S. pyogenes 26689101 Hudson pgi C. glutamicum TGACCGATCATTACTCAAACTTCC...ACCATCTAATTCAACAAGAATT 73224 interfere S. pyogenes 26689101 Hudson xylR C. beijerinckii CGAGTTAGACATAATAGTGA...

  13. Structural Genomics Consortium Plasmids

    Type
    Collection
    ... Plasmid Collections Structural Genomics Consortium Plasmids Structural Genomics Consortium... Consortium Plasmids You may also like... David Waugh Lab plasmids The Structural Genomics Consortium ...available to request. Browse the SGC plasmid collection by All Plasmids SGC Toronto SGC Oxford SGC Stockholm...Stockholm Bacterial Expression Plasmids (empty backbones/controls): ID Plasmid GenBank Key Features 26092 ...Request Plasmids To order, Login or Register a new account. Add the kit or individual plasmids to your...Plasmids from the Structural Genomics Consortium (SCG), which are primarily used for determining protein...the public domain without restriction. Many SGC plasmids from the Toronto, Oxford and Stockholm sites have...

  14. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter...with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In...Ampicillin none, need Cas9 plasmid Qi Do you have suggestions for other plasmids that should be added to ... doi: 10.1016/j.bbrc.2013.08.013. Empty gRNA Expression Vectors Select a gRNA expression plasmid based...CRISPR plasmids that have been designed for use in bacteria. CRISPR...

  15. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... Plasmid Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Empty backbones...Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral...contains AdEasy®-1 plasmid View our Adenovirus Plasmids page for more information Genome...of your cells will take up your plasmid. Thus, many plasmids have markers on them so that you...yeast transformation plasmids with G418 selection Find nematode transgenesis plasmids with Neomycin selection...

  16. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...Pre-constructed pairs of TALEN plasmids targeting zebrafish genes....TCACCGGGAACAATGTGCctgcgtttctgaccaaACTCTGGACGCTGGTGGA id2a TAL3100 & TAL3101 TAACGACGACGGAGCACAgtctgggaatctcgcgGAGCAAGACCCCCGTGGA...
Showing: 21 - 36 of 36 results