We narrowed to 62 results for: cloning
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TypeCollection... Assembly method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease...
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Viral Vectors
TypeCollection...choice (Fig. 1B) . Thus, through standard plasmid cloning, viruses can be engineered to harbor a wide array...easily modified and amplified (through standard cloning methods). There are different types of viruses ... -
TALEN Expression Vectors
TypeCollection...using the REAL, REAL-Fast or FLASH methods) can be cloned for expression as TALENs. Additional details about... -
CRISPR Plasmids - Plants
TypeCollection... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...of Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme Co-expressed Cas9 Cas9 System Selection ... -
Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection
TypeCollection...isoforms and two control vectors. The individual clones, or the pooled library containing the entire pooled... -
Zinc Finger Consortium: Nuclease Expression Vectors
TypeCollection... in-frame to any of these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease... -
Antibody Guide
TypeCollection...of one clone that are specific to a certain epitope, or polyclonal, meaning there are many clones that ...antibodies. Recombinant antibodies are created by cloning antibody genes from specific hybridoma cells into... bind to any given antigen. A mature B cell can clone itself to enable higher production of a single antibody...where each culture should produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies... -
Plasmid Collections
TypeCollection... view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology... -
Bikard Lab - CRISPR Repression Collection
TypeCollection...Dodd, I. B., & Shearwin, K. E. (2013). One-Step cloning and chromosomal integration of DNA. ACS Synthetic... -
COVID-19 Resources
TypeCollection... Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] - Clone mBG17, recombinant mouse monoclonal antibody targeting... Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] - Clone mBG86, recombinant mouse monoclonal antibody targeting... -
Retrovirus Plasmids
TypeCollection...pMSCV PIG (Puro IRES GFP empty plasmid) MSCV For cloning and gene expression; select with puromycin or screen... -
Zinc Finger Consortium: Zinc Finger Arrays
TypeCollection...vectors, DNA encoding zinc finger arrays should be cloned into plasmids also available through Addgene--the... -
Bacterial Expression Systems
TypeCollection...purification tags compatible with Golden Gate modular cloning (MoClo) for use in E. coli . Addgene Blog E. Coli...microscope. The plasmids in this collection are clone-in ready plasmids that contain fluorescent protein... -
Arf GTPase Family
TypeCollection...ARF Superfamily Research You may also like... RAS Clone Collections Richard Kahn Lab Plasmids GTPase Plasmids... -
TALEN Guide
TypeCollection...be used with the recently developed Golden Gate cloning method to assemble “multiple DNA fragments in an... -
Zebrafish Plasmid Collection
TypeCollection...with fluorescent proteins to visualize and track clones of cells over time. Examples of useful tools for... -
CRISPR Plasmids - Mammalian Expression
TypeCollection... based on factors such as selectable marker or cloning method. To isolate mammalian vectors, simply type... -
Cre-lox system
TypeCollection...contain an intron, preventing Cre expression when cloning in E. coli . This alteration enables the generation...deleting the floxed portion of a construct during the cloning process). VCre and SCre are alternative Cre recombinases...infection/transfection efficiency, selecting desired clones, and making transgenic mice. ID Plasmid/Article... -
CRISPR Guide
TypeCollection...researchers to target anywhere from 2–7 genetic loci by cloning multiple gRNAs into a single plasmid — though some... the desired edit experimentally and to isolate clones containing the desired edit (see: Validate genetic...designed in silico and synthesized (Figure 8A), then cloned in a pooled format into lentiviral transfer vectors...PAM sequence in the genomic DNA. This sequence is cloned into a gRNA expression plasmid but does NOT include... -
Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection...chromatin accessibility across different cell types and cloned into an AAV backbone. Most enhancers were derived...