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We narrowed to 62 results for: cloning

Showing: 41 - 60 of 62 results
  1. Zinc Finger Consortium Reagents

    Type
    Collection
    ... Assembly method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease...
  2. Viral Vectors

    Type
    Collection
    ...choice (Fig. 1B) . Thus, through standard plasmid cloning, viruses can be engineered to harbor a wide array...easily modified and amplified (through standard cloning methods). There are different types of viruses ...
  3. TALEN Expression Vectors

    Type
    Collection
    ...using the REAL, REAL-Fast or FLASH methods) can be cloned for expression as TALENs. Additional details about...
  4. CRISPR Plasmids - Plants

    Type
    Collection
    ... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...of Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme Co-expressed Cas9 Cas9 System Selection ...
  5. Antibody Guide

    Type
    Collection
    ...of one clone that are specific to a certain epitope, or polyclonal, meaning there are many clones that ...antibodies. Recombinant antibodies are created by cloning antibody genes from specific hybridoma cells into... bind to any given antigen. A mature B cell can clone itself to enable higher production of a single antibody...where each culture should produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies...
  6. Plasmid Collections

    Type
    Collection
    ... view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology...
  7. COVID-19 Resources

    Type
    Collection
    ... Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] - Clone mBG17, recombinant mouse monoclonal antibody targeting... Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] - Clone mBG86, recombinant mouse monoclonal antibody targeting...
  8. Retrovirus Plasmids

    Type
    Collection
    ...pMSCV PIG (Puro IRES GFP empty plasmid) MSCV For cloning and gene expression; select with puromycin or screen...
  9. Bacterial Expression Systems

    Type
    Collection
    ...purification tags compatible with Golden Gate modular cloning (MoClo) for use in E. coli . Addgene Blog E. Coli...microscope. The plasmids in this collection are clone-in ready plasmids that contain fluorescent protein...
  10. Arf GTPase Family

    Type
    Collection
    ...ARF Superfamily Research You may also like... RAS Clone Collections Richard Kahn Lab Plasmids GTPase Plasmids...
  11. TALEN Guide

    Type
    Collection
    ...be used with the recently developed Golden Gate cloning method to assemble “multiple DNA fragments in an...
  12. Zebrafish Plasmid Collection

    Type
    Collection
    ...with fluorescent proteins to visualize and track clones of cells over time. Examples of useful tools for...
  13. Cre-lox system

    Type
    Collection
    ...contain an intron, preventing Cre expression when cloning in E. coli . This alteration enables the generation...deleting the floxed portion of a construct during the cloning process). VCre and SCre are alternative Cre recombinases...infection/transfection efficiency, selecting desired clones, and making transgenic mice. ID Plasmid/Article...
  14. CRISPR Guide

    Type
    Collection
    ...researchers to target anywhere from 2–7 genetic loci by cloning multiple gRNAs into a single plasmid — though some... the desired edit experimentally and to isolate clones containing the desired edit (see: Validate genetic...designed in silico and synthesized (Figure 8A), then cloned in a pooled format into lentiviral transfer vectors...PAM sequence in the genomic DNA. This sequence is cloned into a gRNA expression plasmid but does NOT include...
Showing: 41 - 60 of 62 results