We narrowed to 44 results for: grna design

1. Zebrafish Plasmid Collection

   Type

   Collection

   ... selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR...promoters, six fluorophores, and empty vectors designed to streamline the production of transgenic zebrafish...

2. Bacterial Expression Systems

   Type

   Collection

   ...see here . pgRNA-bacteria 44251 CRISPR Stanley Qi Expression of customizable guide RNA (gRNA) for bacterial...can be combined with expression of a gRNA from a plasmid like pgRNA-bacteria to knock down endogenous gene...Marraffini Bacterial expression of Cas9 nuclease gRNA. For a more comprehensive list of our bacterial ...molecule, 3OC6HSL, from a promoter repressed by EsaR. Designed to be used with the pAC-EsaR Series . pAC-EsaR...

3. Genomic Deletions in Mammalian Cell Lines

   Type

   Collection

   ...PDF posted at the top of the page. CRISPR Design Design sgRNAs manually or using freely available online... be 24-mer oligos ( Table 4 ). Design Deletion Screening Primers Design one set of primers internal to...not be impacted by a small indel at the sgRNA target site. Design additional primers to analyze for scarring...Here we present a simple methodology for CRISPR design, cloning, and delivery for the production of genomic...ng gDNA, and H 2 O up to 20 μl. Use the primers designed in step 2 above. Conduct PCR for “non-deletion.... Optimize PCR conditions for each primer pair designed based on testing the bulk sorted cells. Run samples...example, sgRNA-A’s protospacer sequence and PAM happen to fall on the bottom (Crick) strand while sgRNA-B’s...

4. Tetracycline Inducible Expression

   Type

   Collection

   ...promoters (sometimes generally called Ptet) have been designed by combining tet O sequences with elements like...as much flexibility and control as a dual vector design, but it is easier to implement and ensures that...Lentiviral Tet-On plasmid for easier cloning and design of inducible shRNA expression, with puromycin selection...104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression of sgRNA TetR H1-2O2 Nathanael...