Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 137 results for: ica

Showing: 31 - 40 of 137 results

  1. Microbiology Resources

    Type
    Collection
    ...MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit design...distributes cannot be used to reconstitute self-replicating microbes or recreate the diseases they cause....our Search page to look for specific genes or applications that are not listed here. Addgene Microbiology...Prions Plasmids for Protozoa Species Entamoeba histolytica Leishmania sp. Plasmodium sp. Toxoplasma gondii...vaccinia virus - Bennink and Yewdell Labs Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly...transcription units in the oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors...fluorescent protein plasmid collection is organized by application and by color. External Resources European Saccharomyces...

  2. Plan Your Experiment

    Type
    Collection
    ...with most experiments, you first need to have a biological question in mind. Then you can decide how to ...component for each category. Genetic Manipulation Application Cas Enzyme Additional Considerations Browse Plasmids...gene you target will depend on your specific application. Knockout : Target constitutively expressed regions...protein and gRNA, which you generally perform by chemical transfection or electroporation. Expression can...systems and delivery methods . Validate Genetic Modification Once you have successfully delivered the gRNA...questions: Does the resulting population need to be identical (clonal) or is a mixed population okay? Does the...the edit need to be homozygous? What type of modification is it? Are you modifying the genomic sequence...

  3. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... by Talley Lambert at Harvard Medical School Zeiss Microscopy : Optical Highlighters NIC Wiki PSFP : Nikon...545-51 Shcherbakova et al. : Journal of the American Chemical Society, May 2012, Vol. 134 No.18, pp. 7913...Nature Communications, November 2012, Vol. 3 No. 1204 Wiedenmann et al. : Journal of Biomedical Optics...Monomer? Background Fluorescent proteins are genetically encoded tools that are used extensively by life... proteins created by Talley Lambert at Harvard Medical School More References below Jump to: Blue/UV Cyan... tagging with a wide variety of colors, some specifically optimized for yeast Lindquist Lab Vectors - ... Vol. 11 No. 5, pp.572-8 Yu et al. : Nature Communications, May 2014 Vol. 15 No. 5, p. 3626 Do you have...

  4. Optogenetics Plasmid Collection

    Type
    Collection
    ...Gene/Insert Vector Type Promoter PI Publication Return to top Optical Switch Plasmids Browse, sort, or search...optogenetics plasmids such as microbial opsin and optical switch tools to measure and manipulate cells and...Fluorescent Protein Guide Microbial Opsin Plasmids Optical Switch Plasmids Background Optogenetics plasmids...proteins. These fusions can then be used as an optical switch to control protein interactions, dimerization...response spectra) of different types of opsins and optical switches available in these tables, refer to Addgene's...Description Gene/Insert Vector Type Promoter PI Publication Return to top Do you have suggestions for other...

  5. Antibody Plasmid Collection

    Type
    Collection
    ...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...and time. An alternative approach is to use synthetically produced recombinant antibodies, created by ...extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant mAb...imaging. Learn more about antibodies and their applications in our Antibody Guide ! Cloning and Expressing...antibodies, and Fc-silent mutants. Functional diversification of hybridoma-produced antibodies by CRISPR/...456(1):527-33. Gavin Wright pET-30-based vector dedicated to efficient scFv expression, which circumvents...and Generation of synthetic nanobodies against delicate proteins. Nat Protoc. 2020 Apr 8. Markus Seeger...

  6. Promega Plasmid Collection

    Type
    Collection
    ...synthetic chemical ligands, which have been developed for special functions that vary from purification, visualization...Promega technologies that support a range of applications. The technology includes NanoLuc, HaloTag, SmBiT...useful for both intracellular and extracellular applications where you want minimal disruption of endogenous...levels in lysates or live-cells, thus preserving physiological relevance. NanoBiT Assays NanoLuc Binary Technology...similar premise, but examine the proximity of a chemical compound and its target protein. The apparent ...Connections (Link opens in a new window) blogs for technical tips and tools for genetic reporter assays (Link...

  7. Retrograde AAV viral preps

    Type
    Collection
    ...55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre and physically-separate mCherry expression Recombinases Deisseroth...55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a Flpo and physically-separate mCherry expression Recombinases Deisseroth...AAV-hSyn1-GCaMP6f-P2A-nls-dTomato Syn GCaMP6f and physically separate nuclear dTomato Calcium sensor Ting ...-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear dTomato, Cre-dependent Calcium...AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate nuclear dTomato Calcium sensor Ting ...sensor Looger 113050 pAAV-hSyn-GRAB_DA1h Syn genetically-encoded fluorescent dopamine(DA) sensor GRAB_...Dopamine sensor Li 123308 pAAV-hSyn-GRAB_NE1m Syn genetically-encoded fluorescent norepinephrine(NE) sensor...

  8. Viral Vectors

    Type
    Collection
    ...required for viral replication. Thus, as a safety feature, viruses used in research typically do not have the...are the most popular virus for gene transfer in clinical trials. These viruses have a relatively high transduction...Overview of Viral Plasmids While plasmids are typically used to encode, propagate, and manipulate genetic...Instead, the genes that are required for viral replication are usually divided among many plasmids, such...plasmids, however, retains the properties of any typical plasmid and thus can be easily modified and amplified... For example, adeno-associated virus (AAV) is typically preferred for in-vivo studies because of its low...

  9. Zhang Lab CRISPR Page

    Type
    Collection
    ... nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single guide...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned scarlessly into the vector...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned into the CRISPR array. The...- sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse the Addgene... genes. It consists of three components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating...function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the Zhang Lab established...can be combined with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin...

  10. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication Plant ID Plasmid ...Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Protocols gRNA Design Tools CRISPR Blog Posts Catalytically dead dCas9, or dCas9 fused to a transcriptional...
Showing: 31 - 40 of 137 results