We narrowed to 93 results for: ple

...total protein content of the sample is determined. Only antibody samples with >90% purity are distributed... webpage. To learn more about these antibodies, please visit the Institute for Protein Innovation Antibody...using a NanoDrop spectrophotometer. If needed, the sample is adjusted by diluting or concentrating to a final...Coomassie staining. For Coomassie staining, the sample is separated via a denaturing SDS-PAGE gel alongside...specific QC experiments were performed on your lot, please email [email protected] . Immunocytochemistry (ICC...

... TetR with the activation domain of the herpes simplex virus VP16 protein (Gossen and Bujard, 1992). This...expression with flexibility for your experiments. For example, you could integrate a transactivator under a tissue-specific... as a dual vector design, but it is easier to implement and ensures that all transfected or transduced...induction, or amount of background expression. For example, only a relatively low level of doxycycline can... ID Plasmid Description Transactivator PI 26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral Tet-On vector... selection rtTA-Advanced Mikhail Alexeyev 17492 pLenti CMV TetR Blast (716-1) Lentiviral Tet-On vector...expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G Tet-...

...infectious viral particle (a virion) are divided among multiple plasmids: Transfer plasmid — contains transgene...plasmid 17618 for GFP reporter. Linzhao Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with...2009) for additional variants. Eric Campeau 39481 pLenti-puro 3rd CMV-driven expression of cDNA with puromycin...-gateway, with puromycin selection. See Root Lab pLEX and pLIX Gateway backbones for additional variants...selection. Kevin Brindle and Duncan Jodrell 17448 pLenti CMV GFP Puro (658-5) 3rd eGFP expression with CMV...of mitochondrial mKeima. Richard Youle 119816 pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase 3rd Expression...

...imaging techniques, including that it is easy to implement due to the simplicity of gRNA design, programmable...for different genomic loci, capable of detecting multiple genomic loci, and compatible with live cell imaging...copies of labeled proteins to the given region. For example, chromosome-specific repetitive loci can be efficiently...visualized in living cells using a single gRNA that has multiple targeted sequences in close proximity. A non-repetitive...genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome painting...

...pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies virus AAV1 Ian Wickersham...pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used to complement deletion-mutant rabies virus AAV1 Ian Wickersham...lacks the ability to infect neighboring cells. For example, G-deleted rabies lacks the viral glycoprotein...infect any neighboring cells. Thus, through complementation, G-deleted rabies virus can spread to monosynaptically...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

... browse the gene list below. For proteins with multiple subunits or isoforms, individual links to each...available plasmids for the gene. For components with multiple subunits or isoforms, individual links to each...EXOC2 EXOC3 EXOC4 EXOC5 EXOC6 EXOC7 EXOC8 Exocyst complex component FGFR FGFR1 FGFR2 FGFR3 FGFR4 Fibroblast... cis/trans isomerase, NIMA-interacting 1 PLXNB1 Plexin B1 PPP1CA Protein phosphatase 1 catalytic subunit...B1 RPTOR Regulatory associated protein of MTOR, complex 1 SAV1 Salvador family WW domain containing protein...

...movement. More than ten million people worldwide are living with PD, mostly people over the age of 60. There...caused by abnormal expansion of a repeating CAG triplet series within the huntingtin (HTT) gene. An individual...variation and lifestyle or environmental factors. For example, variations of Apolipoprotein E (APOE), such as...collection of neurological mouse models including complex and monogenic diseases. Alzforum (Link opens in...

...defined FRT site. Multiplexing: Orthogonal recombinase systems can be combined in multiple, independent recombination...interacts only with their own target sites (for example, Cre with lox sites, Flp with FRT sites, Dre with...preferred codon usage will improve expression. For example, iCre or FLPo in mammalian cells may facilitate...translocation of a gene of interest. Using Cre or Flp as examples, here are a few popular recombinase-dependent ...

...control, including AAV titering by probe-based droplet digital PCR, in vitro and (when possible) in vivo...review performance data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER... serotype will work in your mouse line, see Supplementary Table 3 (Link opens in a new window) . Browse...using the Caltech serotypes in future publications, please acknowledge Viviana Gradinaru and cite as follows...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

... 12 GC/mL for the AAV2 serotype, as titered by droplet digital PCR (ddPCR) . In some cases, we may expect... sequence verification, may not yet have been completed at the time of shipment. If any discrepancies ...for any additional customs fees or taxes, and to complete any additional customs documentation that might...for the import of biological research materials. Please be aware that additional customs charges may be...