We narrowed to 106 results for: Ran;

...editing to easily engineer specific transgenic worm lines. The transparent body of the C. elegans hermaphrodite...Caenorhabditis elegans. Mos1 Based Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit...kit contains 29 plasmids used to generate transgenic nematodes, including C. elegans and C. briggsae. Other...

... educational resources, and efficient material transfer solutions that help advance science while reducing...Promega collection are available in two formats: pretransformed in bacteria as an agar stab 20 µg purified ...specialized Promega technologies that support a range of applications. The technology includes NanoLuc...options. HiBiT technology's exceptional dynamic range can detect low-abundance proteins at endogenous ...NanoBRET (Link opens in a new window) is an energy transfer technique designed to measure molecular proximity...

... used to generate double-stranded breaks ( DSBs or cuts ) or single-stranded breaks ( nicks ). ID Name...Cas9 (dCas9) can be fused to a transactivator and used as a transcriptional activator . ID Name Insert Antibiotic... in a new window) . Please note this does not guarantee viral service, but lets us know what viruses would...

...plasmids encode for microbial opsins, which are membrane channels and pores derived from algae and archaebacteria...ChR2) from Chlamydomonas reinhardtii . ChR2 is a membrane channel that opens in response to blue light and...properties as well as improve the expression and membrane targeting in host cells. Other microbial opsins...cellular activity. For example, search "Cdc42", "transcription", or other terms. ID Plasmid Description Gene...

...non-targeted strand, Cas3 starts unraveling DNA using its helicase properties, cleaving the targeted strand non-specifically...After nicking the target DNA, Cas3 initiates single-strand DNA degradation. This degradation can continue ...non-specifically along the way. Cas3 initiates a final double-strand break, either due to a boundary defined by anti-CRISPR...

...Hockemeyer et al., Nat Biotechnol. 2011 ) TALE transcriptional activators in human cells ( Maeder et al., ...identify both dimeric TALEN and monomeric TALE transcriptional activator target sites and to simplify the ...sequences of interest for potential TALEN or TALE transcriptional activator target sites and then generates individual...Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors for REAL, REAL-Fast...

...mAb Collection . Once the plasmids are transiently transfected into mammalian tissue culture cells, the...making synthetic nanobodies (sybodies) against membrane protein targets. Synthetic single domain antibodies...antibodies for the conformational trapping of membrane proteins. Elife. 2018 May 24;7. and Generation of...

...Loss of the tumor suppressor p53 also promotes aberrant mTORC1 activity, and multiple familial cancers... mTORC1 Complex Symbol Name 4E-BP Eukaryotic translation initiation factor 4E, binding protein 1 Cyclin...Cyclin D1 Also known as CCND1 eIF-4E Eukaryotic translation initiation factor 4E GBL/mLST8 MTOR associated....2014.01.001. PubMed PMID: 24508508 . Targeting the translation machinery in cancer. Bhat M, Robichaud N, Hulea...

...regulators of membrane traffic, they also play critical roles at cilia, centrosomes, the plasma membrane, lipid...2016). Tracing the archaeal origins of Eukaryotic Membrane-Trafficking System building blocks. Molecular ...., Dacks, J. B., Lambright, D. G., Lee, F. S., Randazzo, P. A., Santy, L. C., Schürmann, A., Wilhelmi,...

...listing of transfer status for viral preps.... Viral Vector Packaging Service Penn Vector Core Transfer Viral ...Viral Vector Packaging Service: Penn Vector Core Transfer The University of Pennsylvania Vector Core (Penn...inventory , which includes all viral vectors transferred from the Penn Vector Core. Chemogenetics (including...available in viral format. Please note this does not guarantee viral service, but lets us know what viruses would...

...dependent 1 Francois St-Pierre 179463 pAAV-hSyn-JEDI-2P-Kv-WPRE Syn JEDI-2P none Constitutive 9 Francois St-Pierre...pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE EF1a JEDI-2P none Cre dependent 1 Francois St-Pierre 179460 pAAV-EF1a-DIO-JEDI-2P-WPRE EF1a...pAAV-hSyn-JEDI-2P-WPRE Syn JEDI-2P none Constitutive 9 Francois St-Pierre Voltage Reporter: ASAP 225707 pAAV-hsyn-ASAP5... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

...plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs)....Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence. Two unique ...

...CRISPR plasmids for transcriptional repression of target genes....Catalytically dead Cas9 (dCas9), or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down...down gene expression by interfering with transcription. This interference is most efficient when the gRNA...

...kinase that anchors a signaling network for transitioning between anabolic and catabolic states. Ras Ras...frequently mutated gene in human cancer, is a transcription factor and tumor suppressor. Wnt Coming soon...Tool for Lineage Tracing: The ClonTracer Library (Frank Stegmeier) Tackling Cancers’ Drug Resistance with...

... different DNA concentrations. Can I directly transfect cloning grade DNA into cells? Cloning grade DNA...designed for use in cloning applications. Direct transfection into cells is not recommended. Can I order more...Suggestion Form . Please note that this does not guarantee that Addgene will make cloning grade DNA preps...

...controlling the number of mismatches we can obtain a range of expression levels. This collection comprises ...Together, these plasmids allow to explore the whole range of expression levels for these two reporters simultaneously... S. (2018). Tuning dCas9’s ability to block transcription enables robust, noiseless knockdown of bacterial...

...researchers with powerful tools to investigate neurotransmission, synapse formation, neural connectivity, plasticity...pairs bridge the presynaptic and postsynaptic membranes at neuronal synapses. Image credit: IPI Browse... top Integrins Integrins are a family of mechanotransduction receptors, comprised of alpha-beta subunit...

...the academic community, the labs of Keith Joung, Randall Peterson, and Joanna Yeh are engineering zinc finger... NOTE: All zinc finger arrays listed show transcriptional activation activity of three-fold or [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. References...

...NLS-mCherry or nls-EGFP Cre dependent 1, 2, 5, 8, 9, rg* Brandon Harvey 131002 pAAV-Ef1a-DIO mScarlet EF1a mScarlet... Cre dependent 5 Tim Brown and HHMI-JRC Tool Translation Team 114471 pAAV-Ef1a-fDIO mCherry EF1a mCherry... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...CRISPR-Cas tagging system were tested by tagging transcription factors with FLAG in human cell lines. To repeat...targeting vector that encodes both a guide RNA transcript and a repair template for an individual tagging...