...real-time interrogation and monitoring of brain cells and circuits during behavior. Long desired by researchers...A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human...A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Cre-dependent...genetically encoded dopamine sensor RdLight1 in mammalian cells. Lin Tian 125708-AAV9 pAAV-hSynapsin1-RdLight1 Expresses...neurons Yulong Li 158757-AAV8 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f... Edward Boyden 158757-AAV9 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f...

...expanding the genetic code in bacteria and mammalian cells....codons, and tRNA synthetases are introduced into the cell on plasmids and the new amino acid is introduced...Once these plasmids have been introduced in the cells, the non-canonical amino acid can be incorporated...match the growth medium, ncAA concentration, and the cell lines used previously. Remember that the orthogonal... – GFP for E. coli or mCherry-GFP for mammalian cells. You should also express the protein with and without...tRNA synthetase for use in E.coli and Mammalian Cells. Many of the plasmids also contain one or more copies...

...components or take advantage of existing GFP-lines for cell-specific manipulation. Plasmids Fluorescent Protein...Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Biosensors Optogenetics Background...

...regulators with diverse roles in processes such as cell proliferation, differentiation, inflammation, metabolism...

...listed below have the CMV promoter for mammalian cell expression and a T7 promoter for in vitro transcription...

... time and are potentially heritable in dividing cells. CRISPR Activate and Interfere technologies also...

...is highly recommended that you determine if your cell type contains the target sequence before using any...

...bound to DNA, and the break can be repaired by the cell’s endogenous repair mechanisms. Cas3 belongs to the...

...stable imaging of protein interactions in living cells and live mice. Proteins for in vivo imaging have...Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics Background In vivo ...

... CRISPR plasmids. Each point represents a single cell, and each color represents the population obtained...

...enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs from deleting both...

... engineering in Saccharomyces cerevisiae. Microb Cell Fact. 2013 Oct 25;12:96. doi: 10.1186/1475-2859-...

...then be used to infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in a single...

... inosine, which is treated like guanosine by the cell, creating an A to G (or T to C) change. Adenine ...

...Rangarajan S, Shivalila CS, Dadon DB, Jaenisch R. Cell Res. 2013 Oct;23(10):1163-71. doi: 10.1038/cr.2013.122...

...efficient gene modification. Maeder ML et al. Mol Cell. . 2008. Jul 25. 31(2):294-301. PubMed PMID 18657511...

...Schürmann A, Wilhelmi I, Yohe ME, Kahn RA. Mol Biol Cell. 2019 May 15;30(11):1249-1271. doi: 10.1091/mbc....

...applications like recombinant protein purification and cell-free circuit design in E. coli - Freemont Lab MoClo-YTK...

...and the resulting heteroduplex is resolved by the cell’s mismatch repair (MMR) system. Alternatively, if...

...GCaMP6f-P2A-nls-dTomato Detailed protocol: AAV Production in HEK293 Cells Please share materials by uploading to your Dropbox...