...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited...and denoted as such. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna...Lentiviral BbsI none S. pyogenes Puro, TagBFP Yusa lentiGuide-Puro 52963 Mammalian/Lentiviral BsmBI none S....Mammalian BbsI yes, cut, C-terminal S. pyogenes Zhang pGuide 64711 Mammalian BbsI none S. pyogenes Musunuru ...Mammalian/Lentiviral H1-Tet none S. pyogenes Herold lentiGuide-Crimson 70683 Mammalian/Lentiviral hU6 none S...

...CRISPR guides. The first one binds to sfgfp with either 0, 10, 11, 14, or 20 matching nucleotides, the ...Repression Plasmids You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts...decreasing the expression level. Mismatches between the guide RNA and the target sequence lower the repression...mcherry with either 0, 11, 18, or 20 matching nucleotides. Together, these plasmids allow to explore the...controlled using a plasmid‐borne CRISPR array coding for guide RNAs (diamonds) interspaced with CRISPR repeat motifs...

...phosphate-buffered saline containing 1 mM sodium azide. This buffer is not compatible for use in live cells...sample is separated via a denaturing SDS-PAGE gel alongside serial dilutions of a standard antibody of known... undergoes testing, either in-house or through outside labs, to confirm recognition of the expected target...parental plasmid sequence to confirm the antibody’s identity. For more details on protocols see the Addgene...at room temperature. However, Addgene determines ideal shipping conditions for each order based on the ...you choose, store, or use your antibody. Antibody Guide Antibody Protocols Recipient Instructions Antibody...

...Looking for E. coli or yeast plasmids? Addgene has a wide variety of tools for work in these model organisms... our deposited tools and resources, or read our guide on assembly standards. Browse Plasmids by Microbe...community that aren't in the repository? Help us widen our resources for the community by depositing your...Microbiology General CRISPR Resources Read our CRISPR Guide for a general introduction to CRISPR technology....experiment. Synthetic Biology Assembly Standards Guide Bacterial Expression Systems : Find plasmids to ...bacterial expression system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid...

...to be associated with the disease and implicate a wide range of cellular processes. The vast majority of...affects movement. More than ten million people worldwide are living with PD, mostly people over the age...associated with Alzheimer's disease, through large genome-wide association studies (GWAS) or other large scale ...modification and target species. You can also find our guide to using plasmid pooled libraries . New and Noteworthy...A multicolor atlas for whole-brain neuronal identification in C. elegans. Yemini et al. Cell. 2021 Jan...animal models, purified protein, and more. Plasmids provided by the foundation can be found on our Michael ...

...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence, as well...plasmid. The Mendenhall and Myers labs have also provided their protocol for homology arm cloning: Mendenhall...single plasmid targeting vector that encodes both a guide RNA transcript and a repair template for an individual...epitope tags. The plasmids in the following articles provide PCR templates for amplification of the tag (eg ...Nucleic Acids Res. 2014 The Förstemann lab has provided detailed protocols for N- or C-terminal tagging...

...control methods. Learn More Guide New to using antibodies? In our Antibody Guide, we define key terms, summarize...experts in the field to develop new antibodies and provide experimental data for recommended applications....Interested in our production process? At Addgene, we provide transparency in our production process ...techniques, and explain common applications. View Guide Need Some Help? Browse some of our other resources...

...to use, you have many elements to consider. Here is a guide to Addgene's empty vector backbones. For...A guide to Addgene's empty vector backbones curated by species, epitope tags/fusion protein, selectable...such as Flag or HA, will allow you to easily identify your protein using an antibody against that epitope...Localization See full Fluorescent Protein Guide NLS Nuclear Localization pENTR4-myc-nuc - pEntry...

...activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor peptide, inhibits...parasites, and Xenopus. Addgene CRISPR Resources CRISPR Guide : Essential background information on CRISPR and... CRISPR libraries are a powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag ...

... meeting will be a co-author of the guide. We hope that this guide will improve technology adoption and...the meeting will be a publicly shared practical guide for all scientists in the field and every attendee...lab (main issues/factors that researchers should consider when planning experiments). What resources might...

...subunits, individual links to each gene page are provided below. Color is used for clarity and does not ...subunits, individual links to each gene page are provided below. Symbol Name 14-3-3-σ Stratifin Apaf-1 Apoptotic...apoptosis inducing protein 1 p53R2 p53 inducible, ribonucleotide reductase M2 B (RRM2B) PAG608 Zinc finger, ... PIDD p53-induced death domain protein 1 PIGs Etoposide induced 2.4 PIRH-2 Ring finger and CHY zinc finger...

...Sequencing results are analyzed to confirm the identity and integrity of the viral genome and the absence...Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver...negative staining. Empty vector particles can be identified after negative staining and appear darker than...to the cleared viral preparation pool. Proviral Identity Confirmation and Sterility All of our lentiviral...

... gondii CRISPR genome-wide knockout gRNA pooled library designed with 10 guides against 8,158 predicted...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...

...the time of shipment. If any discrepancies are identified during this analysis, you will be notified by... Addgene performs QC measures which verify the identity of the viral vector, but not the function. Upon... years of the order being shipped, Addgene can provide the QC validation data performed on the material...

...protein interaction screens or cellular assays to identify novel interactions or pathways that are important...Gavin Wright , Wellcome Sanger Institute Protocols: Video showing how AVEXIS works with a detailed protocol...benchmarked protein microarray-based platform for the identification of novel low-affinity extracellular protein...

... to decide which method to use. Addgene's blog post Delivery Methods for Generating iPSCs provides an ... also have the unique potential to continuously divide while maintaining an undifferentiated state. Stem...

...subunits, individual links to each gene page are provided below. Color is used for clarity and does not ...subunits, individual links to each gene page are provided below. mTORC1 Complex Symbol Name 4E-BP Eukaryotic...

...additional pages describing these reagents are provided below: Zinc Finger Genome Engineering Reagents...

...Carcinoid Tumors Bradley Bernstein Broad Institute Identifying Altered Epigenetic States and Drivers in Intestinal...

...mAb Plasmid Collection CRISPR CRISPR-on: an RNA-guided transcriptional activator system - Jaenisch Locus-specific...