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We narrowed to 6 results for: EGFP-Cre cassette

Showing: 1 - 6 of 6 results
  1. Zhang Lab CRISPR Page

    Type
    Collection
    ...backbone with Cre recombinase 60230 : sgRNA targeted to NeuN, plus Cre recombinase-2A-EGFP-KASH 60231 :...pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase...sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Cre recombinase and...sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Cre recombinase and...(backbone)-pCBh-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Cre recombinase and...ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid enables Cre/loxP recombination and...
  2. Lentivirus Plasmids

    Type
    Collection
    ... pSico 3rd Conditional (Cre-lox), stable expression of shRNAs; addition of Cre turns on shRNA expression...more variants. Tyler Jacks 19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion with puromycin resistance. Can ...Expresses shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Luk ...Expresses shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Stephan...expression of EGFP and the gene of interest. Can also express shRNA (cloning H1-shRNA cassettes into the unique...Stephen Elledge 21373 pHIV-EGFP 3rd EF-1alpha driven expression of cDNA and and EGFP co-expression. See plasmid...119816 pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase 3rd Expression of EGFP-Firefly luciferase fusion protein...
  3. CRISPR Plasmids - Tagging

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    Collection
    ...system plasmids for CRISPR-based knock-in of EGFP-2A-PuroR cassette to the C-terminus of endogenous proteins...vector. The PITCh donor plasmid with an EGFP-2A-PuroR cassette, flanked by microhomologous sequences and...deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein, fibrillarin (FBL... lines were created using CRISPR and the donor plasmids containing homology arms and EGFP are available... gRNA pCRIS-PITChv2-FBL - PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus Jorgensen...vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid...cells that uses PCR to generate both an expression cassette for the Cas9-programming sgRNA and HR donors for...
  4. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...blue fluorescent protein 35S-eGFP-nosT - Transient expression vector for eGFP in plants Find FP tagging ...Expression Vectors page Cre-lox Site-specific recombination See our dedicated Cre-lox Plasmids page pBF3060...Lentiviral shRNA expression pLJM1-EGFP - 3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin Hygromycin...scientists often want to create stable cell lines in which the expression cassette of interest is incorporated...Expresses mCherry tag in mammalian cells pBI-MCS-EGFP - Tet-inducible Find more Tet-inducible empty backbones...proteins (GFP, mCherry, etc) Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression pLV-mCherry...including plasmids for constitutive, conditional (Cre-lox), or inducible (Tet) expression miRNA and 3' ...
  5. Zebrafish Plasmid Collection

    Type
    Collection
    ...resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically controlled Cre/loxP system that ...toolbox of genetic methods, such as Tol2 transposons, Cre-mediated site-specific recombination, morpholinos...fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large collections or kits are...Knock-in tagging - Michel Bagnat lab. Targeting cassettes encoding fluorescent proteins for tagging genes...-terminal tags, including Myc, HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible Kit ...that are amenable to genetics, high-throughput screening and non-invasive live imaging. A standard toolbox... Amro Hamdoun Lab. A useful “starter kit” for screening fluorescent protein fusion expression in organisms...
  6. CRISPR References and Information

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    Collection
    ...pCFJ90 (pharyngeal co-injection marker); pDD104 (Cre recombinase) PDF, 105 KB Jaenisch CRISPR plasmid ...cloning All-in-one plasmid containing expression cassette for NmCas9 and BsmBI site for cloning in gRNA:...plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF, 2.4 MB Zhang GeCKO pooled library amplification...packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Pooled libraries are also available for human and...- a CRISPR Screen Tool (Link opens in a new window) Design gRNAs for custom CRISPR screens targeting a...in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's downloadable eBook about...CRISPR pooled libraries for large-scale functional screens. Available for knockout, activation, inhibition...
Showing: 1 - 6 of 6 results