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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11 results for: TOP-GFP reporter element

Showing: 1 - 11 of 11 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...expression vector GFP Varies pMKO.1 GFP - Retroviral shRNA expression pLenti CMV GFP DEST - ...Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression...genome MSCV-IRES-GFP or pMSCV-IRES-mCherry FP - Mammalian retroviral gene expression with GFP or mCherry selectable...genome modification, reporter assays, shRNA expression, transgenics, and more. Element Details Representative...clone your regulatory element or gene of interest to create a luciferase reporter Promoter Measure promoter...different genetic element, or want to express shRNA. Choose by: Species-Specific Expression Epitope Tag or Fusion...Plasmids and Resources collection page Return to top Epitope Tag or Fusion Protein Tags and fusion proteins...

  2. Luciferase Plasmid Collection

    Type
    Collection
    ...Renilla Dual reporter reporter vector allowing the study of biscistronic promoters. Daniel Christophe 119760...clone your regulatory element or gene of interest into to create a luciferase reporter. Unless otherwise ...Multiple Multi-luciferase reporter vector including five transcriptional reporters for NF-kb, TGF-b, c-Myc...luciferase reporter (EF1α-Gaussia luciferase, CMV-Cypridina luciferase). Feng Zhang Return to Top Reporter...know what gene or regulatory element that you need a luciferase reporter for? Browse or use the search...your regulatory element of interest. ID Plasmid Description Gene/Insert PI Return to Top Other Highlighted...Root 105533 pAAV.CMV.Luc.IRES.EGFP.SV40 Firefly CMV AAV expression of firefly luciferase and GFP James Wilson...

  3. Bacterial Expression Systems

    Type
    Collection
    ... Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events...proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression Species PI 14474 pRU1161...Vladislav Verkhusha 52732 52733 pET11a-link-NGFP pMRBAD-link-CGFP GFP (reconstructed) BiFC Lynne Regan 168257... Expression Protein Expression Epitope Tags Protein Tags Return to top Protein Visualization To determine...Fluorescence (GFP+) Gram-negative bacteria Philip Poole 14460 pOT2 Promoter activity Fluorescence (GFPuv) Gram-negative...protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Visualization Protein Interactions Controlled Expression Reporter Plasmids Genome Engineering You may also like…...

  4. Caltech Systemic Capsids

    Type
    Collection
    ...regulatory element dTomato Control Dimidschstein 135631 pAAV-S5E2-GFP-fGFP E2 regulatory element GFP, membrane-targeted...pAAV-CAG-GFP CAG GFP Control Boyden 50465 pAAV-hSyn-EGFP hSyn EGFP Control Roth 50469 pAAV-CaMKIIa-EGFP CamKIIa...104061 CAG-NLS-GFP CAG NLS-GFP Control Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson...regulatory element Activator C1V1 Dimidschstein 135634 pAAV-S5E2-ChR2-mCherry E2 regulatory element Activator...Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden MaCPNS2 These viral vector preparations...Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden CAP-B10 These viral vector preparations...Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden CAP-B22 These viral vector preparations...

  5. Lentivirus Plasmids

    Type
    Collection
    ...CMV drives expression of RFP as a reporter. See plasmid 17618 for GFP plasmid. Cheng 17452 pLenti CMV Puro...hUbC-driven EGFP; can be used for cDNA expression Baltimore 12247 pLVTHM 2nd EF-1a-driven GFP and shRNA ...Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs...Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler...packaging construct encoding Tat. Reiser Return to Top Popular Lentiviral Transfer Plasmids The lentiviral... 11619 pLB 3rd Modification of pLL3.7; Genetic elements known to prevent epigenetic silencing were added...for more versions of this plasmid. Root Return to Top Do you have suggestions for other plasmids that should...

  6. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Dre-dependent plasmids. Return to top Recombinase-Dependent Reporter Plasmids Incorporating a fluorescent...Plasmids Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries/Kits You may also like...recombinases , as well as useful empty backbones or reporters . Experimental Considerations Recombinase Recognition...system allows scientists to utilize recombination elements such as Cre to turn off the expression of one ...DOG New Tricks: Controlling Protein Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and... and Flp-FRT — A Neuroscientist’s View Return to top Flp Recombinase Plasmids ID Plasmid Description Vector... and Flp-FRT — A Neuroscientist’s View Return to top Dre Recombinase Plasmids ID Plasmid Description Vector...

  7. Chemogenetics AAV Preps

    Type
    Collection
    ... 52536 rAAV-CAG::FLEX-rev:: hM4D-2a-GFP hM4D(Gi) - Inhibition GFP Cre-dependent 1 Sternson 154867 pAAV-hSyn-fDIO-hM4D... CAG E2 regulatory element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity Cre-dependent...PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement Serotype PI 44361 pAAV-hSyn-DIO-hM3D...be helpful to the scientific community. Return to top...IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Sternson 119744 AAV CAMKII PSAM4 GlyR IRES EGFP PSAM4...119741 AAV SYN flex PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP Cre-dependent 5, 9 Sternson 119742...PSAM4 GlyR - Inhibition IRES EGFP none 5 Sternson 121538 pAAV SYN1 HA-hM4D(Gi) hM4D(Gi) - Inhibition HA ...

  8. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...50 μm filter into a FACS tube. FACS sort the top ~3% of GFP positive cells in order to enrich for cells...allows genetic perturbation of genes and genetic elements. Here we present a simple methodology for CRISPR...loss-of-function, and utility for the study of non-coding elements. This approach can be used for efficient loss-...loss-of-function studies of genes and genetic elements in mammalian cell lines. Protocol For referenced Tables..., please access the manuscript PDF posted at the top of the page. CRISPR Design Design sgRNAs manually...possible deletion strategies for genes and non-coding elements. For creating a gene knockout, two sgRNA located...sgRNA-B’s protospacer sequence and PAM fall on the top (Watson) strand ( Figure 2A ). However, DSB will ...

  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing...Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca+ dynamics in endoplasmic reticulum GFP-Aequorin Protein...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ... DsRed1-E5 as a reporter for mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial...Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters via electrochromic...Mizushima Autophagy Autophagosome maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation...Clapham Return to Top Cell Physiology Target Description Article PI Autophagy Measure autophagic flux by pH-...

  10. Biosensor AAV Preps

    Type
    Collection
    ...pAAV-Syn-Archon1-KGC-GFP-ER2 Syn Archon1 EGFP Constitutive 8 Boyden 115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] Syn...9 Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent...Syn Archon1 EGFP Cre dependent 8 Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST Syn... Dlx EF1a GFAP/GfaABC1D Synapsin E2 regulatory element Activity Cre-dependent Flp-dependent Cre and Flp-dependent...Voltron2-ST none Cre dependent 1 GENIE Voltage Reporter: JEDI-2P 179459 pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE ...be helpful to the scientific community. Return to top...HaloCaMP1a 138327 pAAV-synapsin-HaloCaMP1a-EGFP Syn HaloCaMP1a EGFP Constitutive 1, 9 Schreiter Calcium Sensor...

  11. CRISPR Guide

    Type
    Collection
    ...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...have evolved in phages and other mobile genetic elements to overcome the CRISPR systems that bacteria and...integrating segments of DNA flanked by specific repeat elements into the genome. CRISPR-associated transposases...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman...
Showing: 1 - 11 of 11 results