We narrowed to 10 results for: TOP-GFP reporter element

...expression vector GFP Varies pMKO.1 GFP - Retroviral shRNA expression pLenti CMV GFP DEST - ...Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression...genome MSCV-IRES-GFP or pMSCV-IRES-mCherry FP - Mammalian retroviral gene expression with GFP or mCherry selectable...genome modification, reporter assays, shRNA expression, transgenics, and more. Element Details Representative...clone your regulatory element or gene of interest to create a luciferase reporter Promoter Measure promoter...different genetic element, or want to express shRNA. Choose by: Species-Specific Expression Epitope Tag or Fusion...Plasmids and Resources collection page Return to top Epitope Tag or Fusion Protein Tags and fusion proteins...

...Renilla Dual reporter reporter vector allowing the study of biscistronic promoters. Daniel Christophe 119760...clone your regulatory element or gene of interest into to create a luciferase reporter. Unless otherwise ...Multiple Multi-luciferase reporter vector including five transcriptional reporters for NF-kb, TGF-b, c-Myc...luciferase reporter (EF1α-Gaussia luciferase, CMV-Cypridina luciferase). Feng Zhang Return to Top Reporter...know what gene or regulatory element that you need a luciferase reporter for? Browse or use the search...your regulatory element of interest. ID Plasmid Description Gene/Insert PI Return to Top Other Highlighted...Root 105533 pAAV.CMV.Luc.IRES.EGFP.SV40 Firefly CMV AAV expression of firefly luciferase and GFP James Wilson...

... Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events...proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression Species PI 14474 pRU1161...Vladislav Verkhusha 52732 52733 pET11a-link-NGFP pMRBAD-link-CGFP GFP (reconstructed) BiFC Lynne Regan 168257... Expression Protein Expression Epitope Tags Protein Tags Return to top Protein Visualization To determine...Fluorescence (GFP+) Gram-negative bacteria Philip Poole 14460 pOT2 Promoter activity Fluorescence (GFPuv) Gram-negative...Fluorescence (GFP:mRFP1 ratio) Escherichia coli Nathan Reyna Additional Addgene Reporter Plasmids Resources...protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...

...regulatory element dTomato Control Dimidschstein 135631 pAAV-S5E2-GFP-fGFP E2 regulatory element GFP, membrane-targeted...pAAV-CAG-GFP CAG GFP Control Boyden 50465 pAAV-hSyn-EGFP hSyn EGFP Control Roth 50469 pAAV-CaMKIIa-EGFP CamKIIa...104061 CAG-NLS-GFP CAG NLS-GFP Control Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson...regulatory element Activator C1V1 Dimidschstein 135634 pAAV-S5E2-ChR2-mCherry E2 regulatory element Activator...Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden MaCPNS2 These viral vector preparations...Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden CAP-B10 These viral vector preparations...Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden CAP-B22 These viral vector preparations...

...drives expression of RFP as a reporter. See plasmid 17618 for GFP reporter. Linzhao Cheng 17452 pLenti ...Sabatini 171123 pLVX-TetOne-Puro-GFP 3rd Dox-inducible expression of GFP. Jason Sheltzer 20342 FUW-M2rtTA...hUbC-driven EGFP. Can be used for cDNA expression. David Baltimore 12247 pLVTHM 2nd EF-1a-driven GFP and shRNA...Expresses shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. ...Expresses shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Stephan...Brindle and Duncan Jodrell 17448 pLenti CMV GFP Puro (658-5) 3rd eGFP expression with CMV promoter and puromycin... 3rd Modification of pLL3.7 with added genetic elements known to prevent epigenetic silencing. Expresses...

...Iwasato 63704 pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre recombinase...Eric Kowarz 96930 XLone-GFP Tet-On PiggyBac vector for inducble expression of EGFP Tet-On 3G rtTA TRE3GS...171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP Tet-On 3G rtTA TRE3GS...variety of improvements. A synthetic Tet Response Element (TRE) typically features seven copies of tet O ...been designed by combining tet O sequences with elements like the H1 RNA promoter. And, most importantly...center: Tet-Off; right: Tet-On. TRE: Tet response element; tTA: tetracycline-controlled transactivator (fusion...activation domain). For simplicity, other promoter elements and transcription machinery are not shown. Tetracycline...

... 52536 rAAV-CAG::FLEX-rev:: hM4D-2a-GFP hM4D(Gi) - Inhibition GFP Cre-dependent 1 Scott Sternson 228902... CAG E2 regulatory element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity Cre-dependent...PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement Serotype PI 44361 pAAV-hSyn-DIO-hM3D...be helpful to the scientific community. Return to top...119741 AAV SYN flex PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP Cre-dependent 5, 9 Scott Sternson... 119742 AAV SYN PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Scott Sternson 119744 AAV...AAV CAMKII PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Scott Sternson 121538 pAAV SYN1...

...Dre-dependent plasmids. Return to top Recombinase-Dependent Reporter Plasmids Incorporating a fluorescent...Plasmids Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries/Kits Site-specific recombinase...recombinases , as well as useful empty backbones or reporters . Experimental Considerations Recombinase Recognition...system allows scientists to utilize recombination elements such as Cre to turn off the expression of one ...DOG New Tricks: Controlling Protein Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and... and Flp-FRT — A Neuroscientist’s View Return to top Flp Recombinase Plasmids ID Plasmid Description Vector... and Flp-FRT — A Neuroscientist’s View Return to top Dre Recombinase Plasmids ID Plasmid Description Vector...

...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing...Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca 2+ dynamics in endoplasmic reticulum GFP-Aequorin Protein...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ... DsRed1-E5 as a reporter for mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial...Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters via electrochromic...Eligible for Viral Packaging PI Return to top Other Reporters ONE-GO Biosensors Kit includes BRET-based...Mizushima Autophagy Autophagosome maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation...

...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...have evolved in phages and other mobile genetic elements to overcome the CRISPR systems that bacteria and...integrating segments of DNA flanked by specific repeat elements into the genome. CRISPR-associated transposases...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman...