Skip to main content
Addgene

We narrowed to 10 results for: cas9 single guide rna sgrna expression vector

Showing: 1 - 10 of 10 results
  1. Zhang Lab CRISPR Page

    Type
    Collection
    ... PX458; SpCas9-2A-EGFP and single guide RNA 62988 : PX459; SpCas9-2A-Puro and single guide RNA 48873 :...and single guide RNA 62987 : PX462; SpCas9n-2A-Puro (D10A nickase) and single guide RNA 48137 : PX165;...using Cas9 with the single guide RNA (sgRNA), which is derived from a synthetic fusion of the CRISPR RNA...codon-optimized SpCas9 or SpCas9n, and the single guide RNA. The vector can be digested using BbsI, and a pair of annealed.... The dual vector system uses S. pyogenes Cas9 (SpCas9), using one vector to express SpCas9, and another...codon-optimized, and packages both the SaCas9 gene and its single guide RNA (sgRNA) into one plasmid (Ran et al....promoter-driven; for sgRNA cloning; has GFP-KASH for FACS sorting SaCas9 + single guide RNA: These plasmids...
  2. Lentiviral Prep Service

    Type
    Collection
    ...proteins can be guided to specific genomic sites by an RNA guide (gRNA) that harbors targeting sequences... 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...backbone. Zhang Cas9 and Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused...Root Cas9 Lentivirus Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins...73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting...73633 Mouse sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro containing...containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting 18,885 ...
  3. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...containing multiple guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette using the...lentiviral vector Mammalian Lentiviral expression of Cas9, dCas9, or dCas9-VP64 along with 1-4 sgRNAs expressed...be used to express multiple gRNAs from a single plasmid with or without expression of Cas9. Highlighted.... Can express Cas9 with up to 3 gRNAs. Frew pSQT1313 Mammalian Single plasmid for the expression of multipe...the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI pCRISPR...meningitidis Joung Cas9 sgRNA vector 68463 Mammalian U6 none S. pyogenes Zhang Cas9/pTREX-n 68708 Other...in which Cas9 has been integrated into the genome. gRNA Empty Vectors Many gRNA empty vectors have been...
  4. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...cellular delivery of a pair of chimeric single guide RNAs (sgRNAs) to create two double strand breaks (DSBs... multiple sgRNAs may be expressed from a single plasmid. Add “CACC” before the 20-mer guide sequence and... the 20-mer is not G. sgRNA expression from the U6 promoter of the pX330 vector is enhanced by the inclusion...plasmid allows for the simultaneous expression of sgRNA and SpCas9, but does not contain markers for selection...example, sgRNA-A’s protospacer sequence and PAM happen to fall on the bottom (Crick) strand while sgRNA-B’s...complement of each guide sequence. Example reverse complement sequences of the Pim1 sgRNA from Table 1 are...and “AAAC” before the guide’s reverse complement for cloning into the pX330 vector using BbsI restriction...
  5. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen... lab Cas9 species = S. pyogenes (PAM = NGG) 47549 pDD162 (Peft-3::Cas9 + Empty sgRNA) R07E5.16 U6 yes,...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator...same time. Single plasmids containing both the gRNA and Cas protein act as all-in-one vectors, but their... to a single category (cut, nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas ...plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab...
  6. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator...base-to-base conversions using a prime editing guide RNA (pegRNA). The pegRNA directs the nickase to the target site...same time. Single plasmids containing both the gRNA and Cas protein act as all-in-one vectors, but their... to a single category (cut, nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas ...
  7. CRISPR References and Information

    Type
    Collection
    ... KB Zhang gRNA cloning CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or...ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H Cas9 ; p201N Cas9 ; pUC gRNA Shuttle PDF, 500 KB Sabatini...sites for S. pyogenes Cas9, S. aureus Cas9, S. thermophilus Cas9, N. meningitidis Cas9, or Cas12a from your...Injection and selection for Cas9-triggered homologous recombination Cas9-sgRNA target construct ; pMA122...injection gRNA core ; Cas9 ; optimized Cas9 PDF, 66.8 KB Fujii gRNA design and cloning gRNA cloning vector...Nematode: Cas9 and gRNA use Cas9 (pIK86) ; gRNA empty backbone (pDR274) PDF, 89 KB Liu Fly: gRNA cloning...DNA FLAG tagged dCas9 PDF, 110 KB Goldstein Nematode: gRNA design and cloning Cas9-sgRNA construct PDF,...
  8. CRISPR Guide

    Type
    Collection
    ...Science Guides CRISPR Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large...components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short...conversions using a prime editing guide RNA ( pegRNA ). Like sgRNA, pegRNA directs the nickase to the target...more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease...transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included in the gRNA-containing...We’ll discuss more Cas9 fusion tools later in the guide. Increasing fidelity of Cas9 Researchers have also... available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements...
  9. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...lentiviral vector for ORF expression pDest-565 - Gateway destination vector for bacterial expression; His,GST...astrocyte-selective expression in AAV Dual promoter Separate expression pRGEB31 - Deliver sgRNA and Cas9 into plants...gene expression vector URA3 Yeast pAG306GAL-ccdB - Yeast Gateway expression vector (see...Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression pLJM1-EGFP - ...hygromycin resistance pRGEB32 - Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter...transient expression vector GFP Varies pMKO.1 GFP - Retroviral shRNA expression pLenti CMV...
  10. CRISPR Plasmids - Tagging

    Type
    Collection
    ...PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific gRNA pCRIS-PITChv2...reaction produces a single plasmid targeting vector that encodes both a guide RNA transcript and a repair...Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen...targeting vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general...uses PCR to generate both an expression cassette for the Cas9-programming sgRNA and HR donors for selectable...components: A vector containing Cas9 and a validated gRNA, based on the Zhang lab's PX458 . Multiple gRNA plasmids...you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence, as well...
Showing: 1 - 10 of 10 results