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We narrowed to 15 results for: cdna expression plasmid

Showing: 1 - 15 of 15 results
  1. Lentivirus Plasmids

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    Collection
    ... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Transfer Plasmids Packaging ...destination plasmid for cDNA expression. Miguel Ramalho-Santos 24129 pULTRA 3rd Bicistronic expression of EGFP... 3rd EF-1alpha driven expression of cDNA and and EGFP co-expression. See plasmid 21374 for dTomato version... VSV-G-expressing envelope plasmid. Bob Weinberg 12259 pMD2.G VSV-G-expressing envelope plasmid. Didier...Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids to express a CRISPR gRNA? Browse our...catalog for lentiviral CRISPR plasmids for gRNA expression . ID Plasmid Generation Description PI 8453...used for cDNA expression. David Sabatini 14883 FUGW 3rd hUbC-driven EGFP. Can be used for cDNA expression...
  2. Open Enzyme Collection

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    Collection
    ... Bacterial Expression Vectors Synthetic Biology Plasmids MoClo Guide Drew Endy Lab Plasmids Jennifer Molloy...enzymes into an expression vector of their choice and then transform into E. coli for expression and purification... and protocols for expression in the Open Bioeconomy Lab's crowd-sourced expression guides (Link opens...Golden Gate assembly. Plasmids Explore the collection and order individual plasmids via the links below:...pOpen-PstIR PstI 165528 pOpen-SfiIR (cDNA) SfiI (used to create cDNA libraries) 165529 pOpen-DpnIR DpnI ...Browse the Open Enzyme collection of plasmids at Addgene for generating enzymes for molecular biology...and codon-optimized the Open Enzymes parts for expression in Escherichia coli . Researchers can use the...
  3. Plasmid Collections

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    ...Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains 167,150 plasmids . We've...Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits ...you are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...Curated Topics Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion...Screening Surface Display cDNA Depositor Collections A modular set of plasmids from a depositor organized...
  4. Cancer Research Plasmids and Resources

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    ...component to find plasmids with your gene of interest. Browse Cancer Research Plasmids by Pathway mTOR ...knockdown, and overexpression constructs. Each collection page listed below organizes the plasmids and resources...Addgene's curated collection of plasmids for cancer research, organized by signaling pathway. Special...Special Collections Cancer Research Cancer Research Plasmids and Resources Addgene has a large variety of resources...Libraries for Cancer Research Broad Target Accelerator Plasmids: Collection of vectors from the Boehm , Hahn ,...vectors from the Sabatini & Wood Labs containing cDNAs for studying oncogenic signaling pathways. ClonTracer...™) to permit construction of RAS pathway gene expression vectors for in vitro and in vivo research. TP53...
  5. Neurodegeneration Research Collection

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    ...Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs...Localization , Optogenetics Plasmids , and Chemogenetics Plasmids . Find plasmids for endogenous gene tagging... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more..... (Bennett et al. Heliyon. 2020) Use VAP expression plasmids to study ER contact with other organelles...Transl Med. 2013) Express human DNMT1 with a Myc tag. (Li et al. J Biol Chem. 2006) Express human ATM with...:2A:Ascl1 (Herdy et al. Elife. 2019) Use plasmids expressing SOX10, OLIG2, and NKX6.2 transcription factors...
  6. CRISPR Plasmids - Tagging

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    Collection
    ...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor... Tagging Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA...the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt.... Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology arms..., which expresses an untagged Cas9 AND a gRNA targeting the AAVS1 locus. Doyon Tagging Plasmid: 3xFLAG...well as pX330S-2-PITCh, expressing a generic gRNA targeting the PITCh donor plasmid. Note that the principle...individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in SapTrap reactions for...
  7. Tetracycline Inducible Expression

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    Collection
    ...Lentiviral Tet-On plasmid for inducible expression of shRNA with puromycin selection. See Plasmid #21916 for ...dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9...Resources Mammalian shRNA Plasmids Cre-lox Plasmids Stem Cell Research Plasmids Blog: Inducible Promoters...gene expression and find plasmids for your experiments using tet- or dox-inducible systems. Plasmid...Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Tetracycline Off Tetracycline...ahead to view highlighted Tet plasmids . Figure 1: Tet-regulated expression systems. Left: natural TetR ...factors to further minimize background expression. Improved expression of the transactivator proteins in mammalian...
  8. Fluorescent Protein Guide: Empty Backbones

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    Collection
    ...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...- Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian...MiCy-N1 - Mammalian Expression MiCy-C1 - Mammalian Expression MiCy-pBAD - Bacterial Expression Return to top...Mammalian Expression sfGFP-C1 - Mammalian Expression sfGFP-pBAD - Bacterial Expression moxGFP 486 510 ...1 - Mammalian Expression mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD - ... - Mammalian Expression Topaz-C1 - Mammalian Expression Topaz-pBAD - Bacterial Expression mTopaz 514 527...- Mammalian Expression mTopaz-C1 - Mammalian Expression mTopaz-pBAD - Bacterial Expression moxVenus 514...
  9. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    Collection
    ...Empty mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag ...localization dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector ...Species-Specific Expression If you want to drive expression of your favorite gene, you will need a plasmid with...EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression, Ligation Independent ...TEV cleavage Transient expression - Gradia Lab plasmids for bacterial expression, Ligation Independent ... Expression - Rubin lab plasmids for Drosophila expression pMT-puro - Inducible...C-terminal GFP for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 ...
  10. Luciferase Plasmid Collection

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    Collection
    ...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...a reference level of expression. Browse or search for popular plasmids expressing luciferase. We also ... preparations of select luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description...Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression Renilla...of luciferase plasmids for gene expression assays and bioluminescent reporters. Plasmid... into a luciferase-containing plasmid and the effects on gene expression can be measured by the luminescence...
  11. TALEN Plasmids and Kits

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    ...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description...-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression of TALORs built... For both plasmids sequence positions 1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting...Maria-Elena Torres-Padilla These plasmids were designed for mammalian expression of fluorescent TAL effectors...pTAL4. The pTAL7 destination plasmids were optimized for TALEN expression in mammalian systems including...TALE Toolbox 49622 - 49647 24 plasmids Thoru Pederson TALEColor plasmids: destination vectors for use ...
  12. Arf GTPase Family

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    Collection
    ... Richard Kahn Lab Plasmids GTPase Plasmids GAP and GEF Plasmids References The ARF (ADP-ribosylation factor...examples of plasmid types available for each gene. Click the gene name for a full list of plasmids available... have plasmids available for request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs. Plasmid...
  13. Retrovirus Plasmids

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    Collection
    ...Gamma-Retroviral Plasmids Gamma-Retroviral Plasmids Transfer Plasmids Packaging Plasmids Envelope Plasmids Additional...MSCV Plasmid for transgene expression with luciferase expression. Scott Lowe 9044 pMIG MSCV Plasmid for... shRNA expression with GFP expression. See plasmid 8452 for puromycin resistance. William Hahn 63704 pRetroX...for transgene expression with GFP expression. See plasmid 12282 for the addition of WRE, a post-transcriptional...Tet-regulated expression of shRNA. Expresses rtTA. See Zuber et al. (2011) for additional plasmids in this ...pLncEXP CMV/MSV lncRNA expression plasmid. Lei Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in ...Use with packaging plasmid. Bob Weinberg 12259 pMD2.G VSV-G-expressing envelope plasmid. Didier Trono 35617...
  14. Genetic Code Expansion

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    Collection
    ...Find GCE synthetase plasmids, target plasmids, and bacterial strains useful for expanding the genetic... Plasmid Collections Genetic Code Expansion Genetic Code Expansion Synthetase Plasmids Target Plasmids...achieve these modifications, two plasmids are generally used. Once these plasmids have been introduced in the...protein translation machinery. A synthetase plasmid expressing the engineered tRNA and its cognate aaRS ... ncAAs. This plasmid often carries multiple tRNA copies for efficiency. A target plasmid containing the...NIH-funded GCE4All Research Center . Synthetase Plasmids Browse plasmids that contain aminoacyl-tRNA synthetases... of these plasmids also contain one or multiple copies of the cognate tRNA gene. ID Plasmid Synthetase...
  15. Fluorescent Proteins: FRET

    Type
    Collection
    ...backbones and control plasmids. Donor Acceptor λ Dex QY D λ Aem ε A QY A R 0 J(λ) Plasmids mTagBFP sfGFP 399.... Browse Addgene's Luciferase Plasmid Collection or the Promega Plasmid Collection to find related tools...Find plasmids and resources for FRET experiments to measure protein interactions or conformation changes... Plasmid Collections Fluorescent Proteins FRET Fluorescent Proteins: FRET FRET Pairs FRET Calibration...FRET on our blog and explore the tables below for plasmids and information to help plan your next FRET experiment...fluorophores through linkers, fluorescent protein expression level and maturation time, and other environmental...The articles or collections listed below feature plasmids with positive and negative FRET controls or calibration...
Showing: 1 - 15 of 15 results