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We narrowed to 6 results for: control shrna

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Showing: 1 - 6 of 6 results

  1. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...deleted. shRNA Expression: Cre-lox can be used to turn shRNA constructs on or off. In floxed-shRNA constructs... excise the shRNA to return gene expression to physiological levels. In lox-STOP-lox shRNA constructs,...systems, how to use them to modify the genome or control gene expression, and find plasmids for your experiments...engineering tools used to precisely manipulate DNA and control the expression of specific genes in cells or organisms...recombinases can be used to modify the genome or control gene expression or skip ahead to browse highlighted...Summary of Cre, Dre, and Flp Recognition Sites. Controlling Recombinase Expression By expressing the enzyme...promoters or inducible systems, you can precisely control and optimize expression of your gene of interest...

  2. Tetracycline Inducible Expression

    Type
    Collection
    ... pAAV-Ptet-RFP-shR-rtTA AAV Tet-On shRNA vector. To evaluate shRNA efficacy by fluorescence, use Plasmid...and repressible promoters are powerful tools to control gene expression and study biological targets. The...popular choices for rapid, efficient, and reversible control of gene expression in eukaryotes. To avoid secondary... While TetR and tet O could be a basic tool to control gene expression, the system has been engineered... TRE: Tet response element; tTA: tetracycline-controlled transactivator (fusion of TetR with VP16 transcriptional...activation domain); rtTA: reverse tetracycline-controlled transactivator (fusion of mutated TetR with VP16...advance was the Tet-Off system. A tetracycline-controlled transactivator (tTA) was created by fusing TetR...

  3. Lentivirus Plasmids

    Type
    Collection
    ...(Cre-lox), stable expression of shRNAs; addition of Cre turns on shRNA expression. See Ventura et al. ... 12247 pLVTHM 2nd EF-1a-driven GFP and shRNA under the control of a tet-responsive H1 promoter. Didier...the gene of interest. Can also express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in...Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging plasmid — ...Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puromycin selection. See plasmid...10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid. Includes a stuffer for easy cloning...cloning. David Root 14748 pLKO.3G 3rd U6-driven shRNA empty plasmid with EGFP marker. See plasmid 14749 for...

  4. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...basal activity shRNA/RNAi Gene silencing Browse RNAi empty vectors for cloning in shRNAs, including plasmids...Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression pLJM1-EGFP...test the function of your gene of interest in a controlled environment under various conditions. When choosing...a different genetic element, or want to express shRNA. Choose by: Species-Specific Expression Epitope ...delivery, genome modification, reporter assays, shRNA expression, transgenics, and more. See the next ...retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral backbones made by Addgene ...uses such as genome modification, reporter assays, shRNA expression, transgenics, and more. Element Details...

  5. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...tdTomato from the EF1 promoter pLL3.7-TNFa-shRNA3 72598 Expresses shRNA against mouse TNF-alpha mRNA from the...vectors to express your gene of interest under the control of a variety of promoters. Professor Oka and the...

  6. Luciferase Plasmid Collection

    Type
    Collection
    ...promoters, enhancers and untranslated regions, or shRNA or CRISPR target sites can be inserted into a luciferase-containing...luciferase gene fusions. Renilla luciferase under the control of a CMV promoter is present for normalization ...Vector Firefly Vector for investigating regions controlling transcription Debrya Groskreutz 18760 MSCV IRES...RapidResponse™ Renilla Vector for investigating regions controlling transcription Pete Stecha 212933 pGL4.82(hRluc...Puro) Renilla Vector for investigating regions controlling transcription Pete Stecha 106292 pLS-SV40-mP-...fusions in plants. Renilla luciferase under the control of a CMV promoter is present for normalization....luciferase. Firefly luciferase expression under the control of a HSV TK promoter for normalization. Modified...
Showing: 1 - 6 of 6 results