...Packaging Contains gag, pol, tat, rev Kasparov 1732 pCPRDEnv 2nd Packaging part of the FELIX vector system, expresses...Scharenberg 12251 pMDLg/pRRE 3rd Packaging Contains Gag and Pol; can be used with 3rd generation transfer plasmids... Rev Trono 12260 psPAX2 2nd Packaging Contains Gag, Pol, Rev, and Tat; can be used with 2nd and 3rd generation...Trono 12263 pCMV delta R8.2 2nd Packaging Contains Gag, Pol, Rev, and Tat; can be used with 2nd and 3rd generation...Weinberg 8455 pCMV-dR8.2 dvpr 2nd Packaging Contains Gag, Pol, Rev, Tat, Vif, and Nef; can be used with 2nd ...expresses Gag-Pol and Rev Nolan 35617 pCAG-Eco N/A Envelope Ecotropic MLV expressing envelope plasmid ...Reiser 17531 pCD/NL-BH*DDD 2nd Packaging Contains Gag/Pol, Tat, Rev. Use with envelope plasmids from Jakob...

...MoMLV gag and pol; use with envelope plasmid Weinberg 12371 pCL-Eco Packaging Contains MoMLV gag, pol,...pol, and env Verma 35614 pBS-CMV-gagpol Packaging MoMLV gag and pol Salmon 8454 pCMV-VSV-G Envelope Envelope...Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an...

...Cre-ERT2 - Tamoxifen inducible; Gateway entry vector none Entry Vector Zon 27493 pBT140 Cre CMV Mammalian Luo...Cre-ERT2;Targeting vector for Fos locus Mammalian Luo 46389 pArcCreER Cre-ERT2;Targeting vector for Arc locus...Burgess 61570 Pvalb-2A-Cre targeting vector Cre; Targeting vector for Pvalb locus Mammalian Zeng 61573...61573 Rasgrf2-2A-dCre targeting vector DHFR-destabilized Cre; targeting vector for Rasgrf2 Mammalian Zeng ...Zeng 61574 Slc17a7-IRES2-Cre targeting vector Cre; Targeting vector for Slc17a7 locus Mammalian Zeng 62219...Nxph4-2A-CreERT2 Targeting Vector Cre-ERT2 - Tamoxifen inducible; Targeting vector Mouse Nxph4 Mammalian Zeng... mCherry T7 Bacterial Sauka-Spengler 119971 GAG-CRErec GAG-Cre fusion; for the production of VLPs loaded...

... within that gene. To construct ZFN expression vectors, DNA encoding zinc finger arrays should be cloned...plasmids also available through Addgene--the specific vector to use depends on the length of the spacer sequence...between the half-sites (see Nuclease Expression Vectors for more details). IMPORTANT NOTE: All zinc finger...OPEN gAGCATCACCtcgctGGAGGTGAGc PTRF/Cavin1 OZ513 and OZ514 OPEN OPEN gACAGCCGTCtgcatctGGAGGTGCCc pitrm1...OPEN OPEN aGACTCCGTCccgtcgtGCTGAGGGCc hnf1a OZ521 and OZ522 OPEN OPEN aACCTTCCCCagagaGAGGTAGTGg VDAC2 OZ523...cACCCTCCTCgtggtGCTGGTGGCc apoeb (apolipoprotein Eb) OZ529 and OZ530 OPEN OPEN gCCCCTCAGCccagatgGGAGGAGATg dpf2 OZ531 and...OPEN OPEN tGTCTCCGGCagaggGAGGAGGAGa esrrga OZ563 and OZ564 CODA CODA tAGCTACCGCTGGCGTCTGAGGAGc HACE1 OZ565...

...oligo when cloning my target spacers into the PX330 vector backbone? There is no need to add the NGG PAM sequence...Recombination (HR) FAQs Can you summarize HR template vector design guidelines? A few notes below are considerations...recently is the double nickase system, with PX335 vector, you need to find two guides for each cleavage ... co-deliver into the cells with the Cas9/sgRNAs vector (like PX330). For large changes (>100bp insertions...typical size (~ 5kb) donor plasmid with Cas9/sgRNAs vector, for one well in 24-well plate. A good example/...typical size (~5kb) donor plasmid with Cas9/sgRNAs vector, for a one well in a 24-well plate. A good reference... PCR and surveyor. I used DNA polymerase Takara Ex Taq ™ DNA Polymerase for my genomic PCR, but couldn't...

...Single polycistronic lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral...-9. Townes Lentivirus Human Single polycistronic lentiviral vector for the expression of human Oct4, Klf4...Lentivirus Human Single polycistronic, doxycycline-inducible lentiviral vector for the expression of human...Jaenisch Lentivirus Mouse Polycistronic, doxycycline-inducible lentiviral vectors for the expression of mouse...murine and human somatic cells using a single polycistronic vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106... Nagy Lentivirus Mouse Polycistronic, doxycycline-inducible lentiviral vectors for the expression of mouse...midbrain dopaminergic neurons using a single polycistronic vector. PLoS One. 2011;6(12):e28719. Gearhart Fibroblasts...

...Reading Frames (ORFs) in pDONR-223 Gateway® Entry vectors from the labs of William Hahn and David Root . ...systems. The MXS-chaining kit includes chaining vectors with multiple cloning sites and standardized building... reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation sequence and flag-epitope...receptor (TCR) engages with antigen presenting cells of the innate immune system. Engagement of the TCR by...class I polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related...catalytic, delta polypeptide p110D PIK3CG phosphoinositide-3-kinase, catalytic, gamma polypeptide PI3CG, PI3K...gonadotropin, beta polypeptide CGB3, hCGB CGB1 chorionic gonadotropin, beta polypeptide 1 - CGB2 chorionic...

...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual... included in the gRNA-containing plasmid, or 2-vector systems, in which Cas9 must be delivered separately...experimental cell population (Figure 8E). In a 2-vector system, you’ll need to either co-infect with a ...based genome engineering from a single lentiviral vector. Nucleic Acids Research , 42 (19), e147. PMID: ...