Zhang Lab CRISPR Page
Type
Collection
...activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator helper complex with 2A GFP 61424 : sgRNA...(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This is a AAV plasmid for sgRNA cloning. GFP-KASH fusion ...PX552; U6 promoter-driven; for sgRNA cloning; has GFP-KASH for FACS sorting SaCas9 + single guide RNA: ... cancer modeling 60224 : sgRNAs targeting KRAS , p53 , and LKB1 , plus luciferase-2A-Cre recombinase, ... . simultaneously modeled the dynamics of KRAS , p53 and LKB1 , the top three significantly mutated genes...the lung generated loss-of-function mutations in p53 and Lkb1 , as well as homology directed repair-mediated...described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR...