...expression of cDNA. Puro selection. Shih 25737 pSLIK-Hygro 3rd Tet-based inducible shRNA or cDNA expression...RD114 Env GP and MLV 4070A Env GP. Good for work in human MSCs. Use packaging construct encoding Tat. Reiser...EGFP 3rd CMV-driven EGFP fusion; can be used for cDNA expression; puro resistance Sabatini 14883 FUGW ...FUGW 3rd hUbC-driven EGFP; can be used for cDNA expression Baltimore 12247 pLVTHM 2nd EF-1a-driven GFP and...plasmid with constitutive or inducible expression of cDNAs, shRNAs or miRNAs, and use a wide variety of drug...3rd Tet inducible gateway destination plasmid for cDNA expression Ramalho-Santos 24129 pULTRA 3rd bi-cistronic...3rd Gateway plasmid for CMV driven expression of cDNA. See article for alternative versions of this plasmid...

...cell. p53 p53, the most frequently mutated gene in human cancer, is a transcription factor and tumor suppressor...vectors from the Sabatini & Wood Labs containing cDNAs for studying oncogenic signaling pathways. ClonTracer...

... pFETCh_Donor CEBPB Human FLAG pFETCh_CEBPB PX458_CEBPB_1 PX458_CEBPB_2 AHR Human FLAG pFETCh_AHR PX458...AHR_1 PX458_AHR_2 ARID4B Human FLAG pFETCh_ARID4B PX458_ARID4B_1 BCL6_iso1 Human FLAG pFETCh_BCL6_iso1 PX458..._BCL6_iso1_2 CEBPG Human FLAG pFETCh_CEBPG PX458_CEBPG_1 PX458_CEBPG_2 RAD21 Human FLAG pFETCh_RAD21 PX458...PX458_RAD21 GABPA Human FLAG pFETCh_GABPA PX458_GABPA_1 PX458_GABPA_2 GABPB1_iso2 Human FLAG pFETCh_GABPB1...iso2 PX458_GABPB1_iso2_1 ATF1 Human FLAG pFETCh_ATF1 PX458_ATF1 CEBPA Human FLAG pFETCh_CEBPA PX458_CEBPA...PX458_CEBPA_2 CREB1 Human FLAG pFETCh_CREB1 PX458_CREB_1 PX458_CREB_2 TGIF2 Human FLAG pFETCh_TGIF2 PX458...PX458_TGIF2_2 SSRP1 Human FLAG pFETCh_SSRP1 PX458_SSRP1_1 PX458_SSRP1_2 ZNF146 Human FLAG pFETCh_ZNF146...

...astrocytes. Endo et al Science. 2022 Nov 4. Base edit human mitochondrial DNA using mitoBEs . Yi et al. Nat ...(Link opens in a new window) such as huntingtin cDNAs, antibodies, and cell lines. Neurodegeneration Method...

...Toolbox PCR/Golden Gate cloning method. Optimized for human expression. Feng Zhang LIC TAL Effector Assembly...ligation-independent cloning (LIC). Validated in human cells. Veit Hornung Musunuru/Cowan Lab TALEN Kit... for deletion mutagenesis and gene targeting in human and mouse pluripotent stem cells. 48706 pTAL7b Joung... TALE repeats (C, T, and G) and the full length human LSD1 at the carboxy-terminus of the protein. 49043...in labeling specific repetitive DNA sequences in human chromosomes Do you have suggestions for other plasmids...rate of Golden Gate assembly. Destination vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression...in cultured cells. Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new destination...

...pCMV-MtPylRS (human-opti) PylRS M. thermophila acetyl-lysine Mammalian Tao Liu 164081 pCMV-MfPylRS (human-opti)...pCMV-MtAcKRS (human-opti) AcKRS M. thermophila acetyl-lysine Mammalian Tao Liu 164196 pCMV-MfBulKRS (human-opti...L-lysine (TFA-Lys) Bacterial TAG Thomas Huber 182287 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF) Y306A/Y384F (AF...PylRS M. mazei Bacterial TAA Wenshe Liu 182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)...

...Aebischer 11442 pBabe bleo human Frataxin FXN Friedreich ataxia Ronald Kahn 11443 pBS human Frataxin (myc tag)...11503 pBabe neo human Frataxin FXN Friedreich ataxia Ronald Kahn 11504 pBabe hygro human Frataxin FXN Friedreich...140575 CaV2.1/pcDNA3.1 ~ Human CACNA1A CMV, T7 Spinocerebellar ataxia 6 Dan Minor 141189 pcDNA4/TO/GFP-GIGYF2... Friedreich ataxia Ronald Kahn 11505 pBabe puro human Frataxin FXN Friedreich ataxia Ronald Kahn 11508...ApoEpromoter APOE ApoE Alzheimer's Sohail Tavazoie 51437 human Alpha-synuclein SNCA T7 Parkinson's Michael J Fox...-Myc-B HTT Myc UbC Huntington's Baoji Xu 110758 Human Parkin (1-465) PRKN His, GST T7 Parkinson's David...CHMP2B mCherry CMV ALS Sanford Simon 115523 MSCV-human CSF-1Rwt-IRES-GFP CSF1R Hereditary diffuse leukoencephalopathy...

...Targeting Gene/Signal Fluorescent Protein PI 71367 Human Beta-catenin GFP Adherens Junctions Beta-catenin...Autophagosome LC3 EGFP Tamotsu Yoshimori 24920 pEGFP-LC3 (human) Autophagosome LC3 EGFP Toren Finkel 14435 mRFP-...Extracellular milieu cfSGFP2-N EGFP Ikuo Wada 73208 pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3 Mitochondria COX IV-derived...Nuc Nucleus NLS mKalama1 Robert Campbell 73205 pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3 Nucleus NLS (from Mak16p...protein) dL5 FAP; mCerulean3 Marcel Bruchez 73207 pcDNA3.1-KozATG-dL5-2XG4S-mCer3 Cytosol, nucleoplasm None...sequence from p63 mTurquoise2 Dorus Gadella 73206 pcDNA3.1-kappa-myc-dL5-2xG4S-TMst Cell surface (mammalian... Peroxisome SKL mNeonGreen Dorus Gadella 73210 pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL Peroxisome SKL ...

... CIneo-RL Renilla CMV Mammalian expression of humanized renilla luciferase Witold Filipowicz 100984 pGL4.18...Nano-lantern/pcDNA3 Nano-lantern CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla... processes, biosensors, or optogenetic tools . pcDNA3.1+-pHlash , pRSETb-pHlash : A pH sensor created ...Gateway cloning. Lentiviral. Jorge Ferrer 87067 pcDNA3.1-ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc...Renilla luciferase is co-expressed. Oskar Laur 87070 pcDNA3.1-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc...fusions using Gateway cloning Mikko Taipale 87065 pcDNA3.1-ccdB-Renilla Renilla Creation of C-terminal Renilla...fusions using Gateway cloning Mikko Taipale 87066 pcDNA3.1-ccdB-Firefly Firefly Creation of C-terminal Firefly...

...the human Estrogen Binding Domain (EBD) TDH3 Yeast van Leeuwen 64770 pSS146 Cre fused to the human Estrogen...122960 pcDNA3.1_PA-Cre photo-activatable Cre recombinase CMV Mammalian Sato 122961 pcDNA3.1_PA-Cre-Y324F...Binding Domain (EBD) Tdh4 Yeast van Leeuwen 65727 pcDNA3.1-CMV-CFP;UBC-Cre25nt Cre with a 25 nucleotide extracellular...