We narrowed to 8 results for: human codon optimized spcas9

...system uses a smaller Cas9 from S. aureus (SaCas9) that has been human codon-optimized, and packages both...vector system uses S. pyogenes Cas9 (SpCas9), using one vector to express SpCas9, and another to express up...plasmids contain two expression cassettes, a human codon-optimized SaCas9 driven by either the ubiquitous cytomegalovirus...) contains a single cassette expressing human codon-optimized SaCas9; SaCas9 sgRNA can be co-transfected...protocol similar to SpCas9 sgRNA PCR (Ran et al., Nature Protocols 2013). SpCas9 Cas9: The PX551 plasmid...MeCP2 promoter (pAAV-pMecp2-SpCas9-spA) for expressing Cas9 in neurons. SpCas9 guide acceptor: The PX552...Return to SpCas9 plasmids GeCKO Library Genome-scale CRISPR-Cas9 knockout screening in human cells. Shalem...

...pAAV-RSV-SpCas9 RSV-driven, constitutive Expression of SpCas9. 8 Hetian Lei 104588 pAAV-EFS-SpCas9 EFS-driven...Engineering Tools These AAV encode Cas9 nuclease used in the CRISPR-Cas9 system for targeted genome editing...CMV-driven, constitutive Expression of human codon-optimized Cas9 from Staphylococcus aureus (SaCas9). ...EFS-driven, constitutive Expression of Myc-tagged SpCas9. 8 Ryohei Yasuda Labeling Tools These AAV encode ...-dependent expression of mKate2 and a truncated human PDE4D3. 1 Georg Nagel 176267 pAAV-FLEX-P301L Tau...EF1a-driven, Cre-dependent Cre-dependent expression of human 2N4R microtubule-associated protein tau with P301L...

...lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin...Selection PI Human knockout pooled libraries 73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA...This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs targeting 19,114 human genes along with 1000...Root Cas9 Lentivirus Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins...backbone does not contain SpCas9. 76,441 unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting...Doench , David Root 73179 Human gRNA library Brunello in lentiCRISPRv2 Human sgRNA library in backbone...Doench , David Root 247026 Human sgRNA library Jacquere in pMV_AA050 Human sgRNA library in backbone pMV_AA050...

...inhibit MMR PEmax — optimized PE2 enzyme containing RT optimized for human codons, additional nuclear ...systems PE6 — small prime editors with optimized RT and/or Cas9 domains PE7 — addition of RNA-binding ...contains Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection...transcriptase (RT) is fused to the C terminus of Cas9 H840A nickase. The fusion enzyme is capable of installing...template. During prime editing, the pegRNA directs the Cas9 nickase to the target sequence where it nicks the...nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can be used in PE2–PE5...PuroTK-pegRNA_GG Mammalian, piggyBac hU6 pegRNA BsmBI Yes (Cas9 H840A + MMLV RT) PuroTK Jacob Giehm Mikkelsen 174038...

...recombination of the DNA between target sites. Codon optimization: Modifying the DNA sequence of the recombinase...integration efficiency but research groups continue to optimize these tools. The best characterized SSR systems...inducible systems, you can precisely control and optimize expression of your gene of interest. Inducible...be fused to a ligand-binding domain such as the human estrogen receptor (ERT2) so that the enzyme is only...recombinase to align with your organism's preferred codon usage will improve expression. For example, iCre... expression strategies. Gene Expression: A stop codon flanked with loxP sites (often called a "lox-STOP-lox...absence of Cre. In the presence of Cre, the stop codon is excised, and gene expression proceeds. Gene Knockout...

...strand SpCas9-HF1 — disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 — increase Cas9 proofreading...application. Cas9 Orthologs While SpCas9 is the most commonly used Cas enzyme, additional Cas9 orthologs ...Plasmids: Purify Cas9 Alternatives for CRISPR Genome Engineering S. pyogenes Cas9 (SpCas9) is the most commonly...Species/Variant of Cas9 PAM Sequence* Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant 3...reduced NAG binding) SpCas9 VRER variant 3' NGCG SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN ...Edits Large Edits Genome-Wide Screening Cas9 Fusion Tools Cas9 Alternatives Resources Plan Your Experiment...positively-charged grooves on Cas9. gRNA binding induces a conformational change in Cas9, shifting it into an ...

...proteins in mammalian cells or other species by codon optimization of their sequence and eliminating interactions...Resnitzky et al., 1994). TRE3G promoter : also optimized into P TRE3GV for viral expression and P TRE3GS... 87360 TLCV2 Lentiviral vector for tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam...51543 FUW-tetO-hOKMS Tet-On inducible expression of human Oct4, Sox2, Klf4, and Myc for iPS cell generation...differentiating iPSCs into glutamatergic neurons via human NGN2 expression. See more from the iPSC Neurodegenerative...pUCM-AAVS1-TO-hNGN2 Introduction of dox-inducible human NGN2 into AAVS1 safe harbor site, for iPSC differentiation...Klaver, B., Berkhout, B., & Das, A. T. (2006). Optimization of the Tet-On system for regulated gene expression...

...Gene Type Wildtype Mutant Partial Sequence Codon-Optimized gRNA Other Tags GFP FP other Flag GST His TEV...11503 pBabe neo human Frataxin FXN Friedreich ataxia Ronald Kahn 11504 pBabe hygro human Frataxin FXN Friedreich...-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP U6 ALS...shSOD1mismatch SOD1 H1 ALS Patrick Aebischer 11443 pBS human Frataxin (myc tag) FXN Myc T7 Friedreich ataxia ... Friedreich ataxia Ronald Kahn 11505 pBabe puro human Frataxin FXN Friedreich ataxia Ronald Kahn 11508...ApoEpromoter APOE ApoE Alzheimer's Sohail Tavazoie 51437 human Alpha-synuclein SNCA T7 Parkinson's Michael J Fox...-Myc-B HTT Myc UbC Huntington's Baoji Xu 110758 Human Parkin (1-465) PRKN His, GST T7 Parkinson's David...