We narrowed to 8 results for: human sgrna library

...73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting...Doench , Root 73179 Human gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2...Puromycin Doench , Root Human activation pooled libraries 92379 Human CRISPRa sgRNA library Calabrese in backbone...knockout pooled libraries 73633 Mouse sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone..., Root Human inactivation pooled libraries 92385 (Discontinued) Human CRISPR inhibition library Dolcetto...Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled libraries 73178 ...backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) targeting...

...Pooled genome-wide human SAM library SAM can be combined with a human genome-wide library to activate all...top GeCKO Screening Library GeCKO are pooled lentiviral CRISPR libraries (both human and mouse) for performing...described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR...contains a single cassette expressing human codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons...screens. The libraries are available in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together...lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse the Addgene GeCKO Library CRISPR page for more information...has been human codon-optimized, and packages both the SaCas9 gene and its single guide RNA (sgRNA) into ...

...Addgene blog. CRISPR Pooled Libraries Addgene's collection of CRISPR pooled libraries for large-scale functional...CRISPRko/a/i sgRNA sequences to maximize on-target activity for selected target(s). Uses human, mouse, and...protocol Addgene CRISPR pooled library amplification CRISPR pooled libraries Protocol at Addgene Church gRNA... Sabatini and Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF, 129 KB Sontheimer gRNA design and cloning...positive control: CMV-EGFP Pooled libraries are also available for human and mice PDF, 276 KB Content last...plasmids in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's downloadable ... can identify positively or negatively selected sgRNAs, genes, or pathways. For use with genome-scale ...

... lentiviral pooled libraries . Browse Libraries: CRISPR Pooled Libraries Cas9 Fusion Tools dCas9 cannot...lentiviral CRISPR libraries. Figure 8: Overview of genome-wide screening using CRISPR libraries. What Are Pooled...Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous population ..., so CRISPR libraries contain thousands of unique gRNAs. gRNA design for CRISPR libraries is usually optimized...activation and repression libraries target promoter or enhancer regions. As each library is unique, be sure ...sure to check the library information and original publication to see if a library is suitable for your experiment...CRISPR libraries have been developed for inhibition using dCas9. How Do You Use a CRISPR Library? CRISPR...

... pFETCh_Donor CEBPB Human FLAG pFETCh_CEBPB PX458_CEBPB_1 PX458_CEBPB_2 AHR Human FLAG pFETCh_AHR PX458...AHR_1 PX458_AHR_2 ARID4B Human FLAG pFETCh_ARID4B PX458_ARID4B_1 BCL6_iso1 Human FLAG pFETCh_BCL6_iso1 PX458..._BCL6_iso1_2 CEBPG Human FLAG pFETCh_CEBPG PX458_CEBPG_1 PX458_CEBPG_2 RAD21 Human FLAG pFETCh_RAD21 PX458...PX458_RAD21 GABPA Human FLAG pFETCh_GABPA PX458_GABPA_1 PX458_GABPA_2 GABPB1_iso2 Human FLAG pFETCh_GABPB1...iso2 PX458_GABPB1_iso2_1 ATF1 Human FLAG pFETCh_ATF1 PX458_ATF1 CEBPA Human FLAG pFETCh_CEBPA PX458_CEBPA...PX458_CEBPA_2 CREB1 Human FLAG pFETCh_CREB1 PX458_CREB_1 PX458_CREB_2 TGIF2 Human FLAG pFETCh_TGIF2 PX458...PX458_TGIF2_2 SSRP1 Human FLAG pFETCh_SSRP1 PX458_SSRP1_1 PX458_SSRP1_2 ZNF146 Human FLAG pFETCh_ZNF146...

...104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression of sgRNA TetR H1-2O2 Nathanael...Takeshi Imai 155257 Watermelon Pooled Library Lentiviral barcode library for simultaneous tracing of clonal...51543 FUW-tetO-hOKMS Tet-On inducible expression of human Oct4, Sox2, Klf4, and Myc for iPS cell generation...differentiating iPSCs into glutamatergic neurons via human NGN2 expression. See more from the iPSC Neurodegenerative...pUCM-AAVS1-TO-hNGN2 Introduction of dox-inducible human NGN2 into AAVS1 safe harbor site, for iPSC differentiation...

...editing. These edits can include mutations seen in human patients, protein tags, or loxP/FRT sites, among...especially amenable to genetic screens. Pooled gRNA libraries can be used to identify genes that are important...important to a given phenotype. Current libraries are available for gene knockout, as well as transcriptional...germline editing, with the International Summit of Human Gene Editing convened in 2015 to discuss the ethics...plasmids for the following models: Mammalian systems (Human, mouse, and rat) Bacteria ( E. coli , Streptococcus...2013. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell . ... Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep . 13(3):621-33. PMID: 26456817 Fu...

...plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging ...RD114 Env GP and MLV 4070A Env GP. Good for work in human MSCs. Use with second-generation packaging construct...Guide Addgene’s Viral Service Lentiviral Pooled Libraries All Viral Plasmids Viral Vectors 101 eBook Biosafety...