...antibodies distributed by Addgene have been produced in-house by Addgene scientists. They are generated using ...Protein band intensities are measured using ImageJ software and a standard curve is generated. The final antibody...calculated from the standard curve. Using ImageJ software, the band intensities of both the antibody and...Each lot of antibody undergoes testing, either in-house or through outside labs, to confirm recognition ...

...cells by size and signals in the flow cytometry software. Flow sample cells, one at a time, through the...two antibodies to upwards of twenty. Laser and software capacity theoretically allow for roughly fifty...as GAPDH or tubulin (sometimes referred to as housekeeping genes). This allows for relative comparison ...

... Addgene CRISPR 101 eBook (3rd edition) CRISPR software and depositor protocols JOVE video reprint: Protocol...every target gene, so CRISPR libraries contain thousands of unique gRNAs. gRNA design for CRISPR libraries...Ter-Ovanesyan, D., Braff, J. L., Davidsohn, N., Housden, B. E., Perrimon, N., Weiss, R., Aach, J., Collins...