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Showing: 1 - 12 of 12 results

  1. Lentiviral Prep Service

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    ...Root Cas9 Lentivirus Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,...function, see our CRISPR plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits,...Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses are made from plasmids... 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...GFP Zhang 61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding...Packaging Service page . Pooled CRISPR Libraries for Screening Use pooled CRISPR libraries to screen for genes...

  2. CRISPR Plasmids - Empty gRNA Vectors

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    ...Whether the plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that can ... CRISPR Empty gRNA Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR requires...Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI pCRISPR 42875... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...from our collection. Plasmids Expression System Description PI Multiplex CRISPR/Cas9 Assembly System Kit...independent U6 promoters and Cas9 from CBh promoter. Ventura A CRISPR/Cas9 toolkit for multiplex genome...

  3. CRISPR Guide

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    ...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...genomic regions using CRISPR Browse Plasmids: Purify Cas9 Alternatives for CRISPR Genome Engineering S....References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software and...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening... containing the CRISPR plasmid will have all desired genomic edits carried out by Cas9. Multiplexing applications...more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease...

  4. Zhang Lab CRISPR Page

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    ...PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice for genome editing...backbone cloning information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors ...Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery AAV - Cas9 Mouse CRISPR... 24. PubMed . Return to SpCas9 plasmids GeCKO Library Genome-scale CRISPR-Cas9 knockout screening in human...CRISPR Cas9 gRNA plasmids from the Zhang lab Genome... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang...Zhang Lab CRISPR Plasmids Available from Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the ...

  5. CRISPR References and Information

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    ... cloning CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335...ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H Cas9 ; p201N Cas9 ; pUC gRNA Shuttle PDF 488.6 KB Sabatini and..... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts In collaboration with the labs who...deposited CRISPR plasmids , we've created a series of links and guides to help you use CRISPR in your lab...KB Church gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF 104.1 KB Chen and Wente Zebrafish...PDF 102.5 KB Jaenisch CRISPR plasmid summary for transcriptional activation dCas9 activators sgRNA dual...-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF 47.5 KB Vosshall and Matthews CRISPR/Cas9 reagent generation;...

  6. Plan Your Experiment

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    ... started using CRISPR in your research. Although we will use the example of CRISPR/Cas9 in mammalian cells...the multi-plasmid SAM system. Select Expression System To use CRISPR, you will need both Cas9 and a gRNA... specific plasmid in question (see CRISPR protocols from Addgene depositors ). Deliver Cas9 and gRNA Choose... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan Your Experiment...organism without a specific preferred mutation Cas9 (or Cas9 nickase) Single (or dual) gRNA targeting 5′ ...generating a point mutation or inserting a tag Cas9 (or Cas9 nickase); Base editor Single (or dual) gRNA ...express the CRISPR machinery. For more difficult cells (e.g. primary cells), viral delivery of CRISPR reagents...

  7. Qi Lab CRISPR Page

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    ...bacterial Cas9 expression plasmid 44250 pwtCas9-bacteria A wild-type Cas9 expression plasmid for use editing... CRISPR Qi Lab CRISPR Plasmids Qi Labs CRISPR Plasmids...CRISPR CRISPRi Cas9 gRNA plasmids from the Qi lab CRISPR...Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts We have recently...Individual plasmids can be ordered via the links below: ID Plasmid Description 44246 pdCas9-humanized ... inactive, human codon-optimized Cas9 expression plasmid 44247 pdCas9::BFP-humanized A catalytically inactive... expression plasmid for use with either human codon-optimized Cas9 constructs 44249 pdCas9-bacteria A ...

  8. Fujii Lab CRISPR Plasmids

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    ... Fujii Lab CRISPR Plasmids Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA...CRISPR Cas9 gRNA plasmids from Dr. Hodaka Fujii's lab. Genome...scheme Fujii Lab Plasmids Individual plasmids can be ordered via the links below: ID Plasmid Description 47948...proteins and the CRISPR system consisting of a catalytically inactive form of Cas9 (dCas9) plus guide RNA... Genome Engineering CRISPR Fujii Lab CRISPR Plasmids...elements/pMD20 iChIP plasmid, LexA-binding elements 48874 3xFNLDD/pCMV-7.1 iChIP plasmid, expresses 3xFNLDD...-I2 iChIP plasmid, expresses 3xFNLDD in mammalian cells 48972 3xFNLDD/pMXs-IG iChIP plasmid, expresses...

  9. CRISPR Pooled gRNA Libraries

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    ... CRISPR Pooled Libraries CRISPR Pooled Libraries Browse CRISPR Plasmids By Function Genome...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is ...primary cells). Most CRISPR libraries require the use of lentivirus to deliver the gRNA/Cas9 to target cells...Human CRISPRi Non-coding Libraries 86538 — 86550 Inhibition Human Weissman 3rd 10 Varies CRISPR/Cas9-assisted...virtually any genetic locus. CRISPR pooled libraries consist of thousands of plasmids, each containing multiple...experiments using a pooled CRISPR library are far more complex than using CRISPR to modify a single genomic...steps for using pooled CRISPR libraries and keep the following in mind: CRISPR screening experiments require...

  10. Neurodegeneration Research Collection

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    ... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...Dhekne et al. Elife. 2023 Oct 24. See More CRISPR Tools Find CRISPR pooled libraries available from Addgene...Target neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent rodent...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...find our guide to using plasmid pooled libraries . New and Noteworthy: Use a CRISPRi system to target alpha-synuclein...

  11. CRISPR Plasmids and Resources

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    ...preps of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used ...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...you need to use CRISPR in your own lab. Check out our CRISPR resources or browse plasmids below by function...Xenopus. Addgene CRISPR Resources CRISPR Guide : Essential background information on CRISPR and the basics...planning your first CRISPR experiment. CRISPR 101 eBook : Based on our popular CRISPR 101 blog series, we've...analysis, discover links to CRISPR forums, and more. CRISPR History : Learn how CRISPR was modified from a bacterial...genome engineering. CRISPR Topic Page : Want the latest news on CRISPR? Experts cover CRISPR topics on Addgene's...

  12. Neurodegeneration Plasmid Collection

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    ...Neurodegeneration Neurodegeneration Plasmids Neurodegeneration Plasmid Collection Addgene has a large collection...Bonifacino 173579 Lenti-sgAtm#1/Cre ATM CMV Ataxia telangiectasia Charles Swanton 173580 Lenti-sgAtm#2/Cre ...Parkinson's Frank Soldner 180437 Px330-EGFP-LRRK2-CRISPR/Cas9 LRRK2 U6 Parkinson's Frank Soldner 180439 STR-KDEL_PGRN-SBP-mCherry...Browse all Neurodegeneration Plasmids Do you have suggestions for other plasmids that should be added to this...Flag GST His TEV Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast...sgHTT1-7sk-sgCas9 HTT U6, 7sk Huntington's Nicole Deglon 190901 pAAVss-U6-sgHTT51-7sk-Cas9 HTT U6, 7sk...Browse neurodegeneration plasmids for research related to Alzheimer's Disease, ALS, Frontotemporal Dementia...
Showing: 1 - 12 of 12 results