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We narrowed to 9 results for: lenti sgrna

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Showing: 1 - 9 of 9 results

  1. Lentiviral Prep Service

    Type
    Collection
    ...73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting...73633 Mouse sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro containing...Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation...containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting 18,885 ...GFP Zhang 61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding...gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes...targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Mammalian lenti sgRNA(MS2)_zeo backbone - 3rd gen lenti sgRNA cloning backbone with MS2 loops at tetraloop...Mammalian pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-blast...selectable marker pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral...- Deliver sgRNA and Cas9 into plants by Agrobacterium-mediated transformation Dual-sgRNA_hU6-mU6 - Lentiviral...Lentiviral expression plasmid for two sgRNAs from an hU6 and an mU6 promoter Protein-protein interaction...confers hygromycin resistance pRGEB32 - Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ... genome pLenti CMV GFP DEST - Lentiviral Gateway destination vector for gene expression pLenti CMV/TO ...

  3. Lentivirus Plasmids

    Type
    Collection
    ...plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging ... safety. Lentiviral Vector Guide Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids ...at Addgene. Read our Lentiviral Vector Guide for more information about lentiviral components, generations... Additional Resources Lentiviral Vector Guide Addgene’s Viral Service Lentiviral Pooled Libraries All ...Addgene lentiviral packaging, envelope, and transfer plasmids Viral... Viral Plasmids Lentiviral Plasmids Lentiviral...Packaging Plasmids Envelope Plasmids Resources Lentiviruses, a subset of retroviruses, are some of the most...

  4. Zhang Lab CRISPR Page

    Type
    Collection
    ...described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR...CMV-driven SaCas9; U6-driven sgRNA 61593 : PX602; TBG-driven SaCas9; U6-driven sgRNA 61592 : PX600; CMV-driven... SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran... pAAV-U6::sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This is a AAV plasmid for sgRNA cloning. GFP-KASH...implemented by delivering Cre and sgRNA to a Cre-dependent mouse or sgRNA to a constitutively Cas9-expressing...control for AAV-KPL; sgRNA targeted to LacZ, plus luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone...genome editing 60227 : sgRNA targeted to NeuN, plus Cre recombinase 60228 : sgRNA targeted to LacZ, plus...

  5. CRISPR References and Information

    Type
    Collection
    ... Sabatini and Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF, 129 KB Sontheimer gRNA design and cloning...batch (Link opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new window...or Cas9 D10A (pX335) PDF, 255 KB Zhang sgRNA cloning lentiCRISPR v2 packaging plasmids: pVSVg , psPAX2 ... can identify positively or negatively selected sgRNAs, genes, or pathways. For use with genome-scale ... and a ctivation (Link opens in a new window) A sgRNA design tool for genome editing, as well as gene ...Link opens in a new window) Identifies candidate sgRNA target sites by off-target quality. Validated for... (Link opens in a new window) Identification of sgRNA target sites and off-target evaluation for base ...

  6. Luciferase Plasmid Collection

    Type
    Collection
    ...luciferase Scott Lowe 105621 Lenti-luciferase-P2A-Neo Firefly EF1α Lentiviral expression of firefly luciferase...Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla...fusions using Gateway cloning. Lentival. Mikko Taipale 87078 pLenti6.2-Nanoluc-ccdB NanoLuc® Creation ...transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase...luciferase Eric Campeau 21474 pLenti CMV V5-LUC Blast (w567-1) Firefly CMV Lentiviral expression of firefly luciferase...Firefly Ubc Lentiviral expression of firefly luciferase Linzhao Cheng 74444 pLenti.PGK.blast-Renilla_Luciferase...Marcel Bruchez 108542 pLenti-EF1a-Luciferase-IRES-Blast-WPRE Firefly EF1α Lentiviral expression of firefly...

  7. Tetracycline Inducible Expression

    Type
    Collection
    ...104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression of sgRNA TetR H1-2O2 Nathanael...Description Transactivator PI 26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral Tet-On vector with blasticidin...rtTA-Advanced Mikhail Alexeyev 17492 pLenti CMV TetR Blast (716-1) Lentiviral Tet-On vector expressing TetR ...87360 TLCV2 Lentiviral vector for tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam ...Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for inducible expression of shRNA...2O2 Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design ...rtTA3 Ptet, miniCMV Howard Gu 44012 pInducer20 Lentiviral Tet-On vector for inducible expression. See article...

  8. CRISPR Guide

    Type
    Collection
    ...systems contain two components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme...using a prime editing guide RNA ( pegRNA ). Like sgRNA, pegRNA directs the nickase to the target site by...efficiency PE3 — increased efficiency; an additional sgRNA directs Cas9n to a nearby site, driving mismatch...in nature and is also commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the ... D. E. (2014). Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation. Nature...labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nature Biotechnology . 34 (... libraries. What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...

  9. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Walker Jackson 78624 sgRNA(MS2)_Prnp_SAM1 PRNP U6 Dementia Walker Jackson 78625 sgRNA(MS2)_Prnp_SAM2 PRNP...Hyman 87200 pX335 HTT sgRNA-b HTT U6 Huntington's Mahmoud Pouladi 87201 pX335 HTT sgRNA-a HTT U6 Huntington's...Bonifacino 173579 Lenti-sgAtm#1/Cre ATM CMV Ataxia telangiectasia Charles Swanton 173580 Lenti-sgAtm#2/Cre ...LRRK2 His T5 Parkinson's Suzanne Pfeffer 208392 Lenti-sgRNA-Cre-GpNLuc-sgmATM ATM Ataxia telangiectasia Gaorav...Venus CMV Lewy body dementia Tiago Outeiro 89643 Lenti-sgAtm/Cre ATM U6 Ataxia telangiectasia Monte Winslow...FusionRed CUP1 ALS Michihiko Kataoka 188460 pKDR-Lenti-CBh.6-SOD1 SOD1 CBh ALS Erik Dent 188572 Tet-off...Cookson 13318 pLenti6-DEST PINK1-V5 G309D PINK1 V5 CMV Parkinson's Mark Cookson 13319 pLenti6-DEST PINK1-...
Showing: 1 - 9 of 9 results